PP296_ARATH
ID PP296_ARATH Reviewed; 960 AA.
AC Q9M1V3; F4J0Z5;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 2.
DT 25-MAY-2022, entry version 115.
DE RecName: Full=Pentatricopeptide repeat-containing protein At3g63370, chloroplastic {ECO:0000305};
DE AltName: Full=Protein ORGANELLE TRANSCRIPT PROCESSING 86 {ECO:0000303|PubMed:19934379};
DE Flags: Precursor;
GN Name=PCMP-H83; Synonyms=OTP86 {ECO:0000303|PubMed:19934379};
GN OrderedLocusNames=At3g63370; ORFNames=F16M2_220;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=15269332; DOI=10.1105/tpc.104.022236;
RA Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C.,
RA Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M.,
RA Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B.,
RA Taconnat L., Small I.;
RT "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins
RT reveals their essential role in organelle biogenesis.";
RL Plant Cell 16:2089-2103(2004).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=19934379; DOI=10.1105/tpc.109.071472;
RA Hammani K., Okuda K., Tanz S.K., Chateigner-Boutin A.L., Shikanai T.,
RA Small I.;
RT "A study of new Arabidopsis chloroplast RNA editing mutants reveals general
RT features of editing factors and their target sites.";
RL Plant Cell 21:3686-3699(2009).
CC -!- FUNCTION: Involved in RNA editing event in chloroplasts. Required for
CC the editing of a single site in rps14 transcript.
CC {ECO:0000269|PubMed:19934379}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:19934379}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions. {ECO:0000269|PubMed:19934379}.
CC -!- SIMILARITY: Belongs to the PPR family. PCMP-H subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB86438.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=Pentatricopeptide repeat proteins;
CC URL="https://ppr.plantenergy.uwa.edu.au";
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DR EMBL; AL138648; CAB86438.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE80472.2; -; Genomic_DNA.
DR PIR; T48126; T48126.
DR RefSeq; NP_001319827.1; NM_001340211.1.
DR PDB; 7O4E; X-ray; 2.50 A; A/B=826-960.
DR PDB; 7O4F; X-ray; 1.65 A; A/B/D/G=826-960.
DR PDBsum; 7O4E; -.
DR PDBsum; 7O4F; -.
DR AlphaFoldDB; Q9M1V3; -.
DR SMR; Q9M1V3; -.
DR STRING; 3702.AT3G63370.1; -.
DR PaxDb; Q9M1V3; -.
DR PRIDE; Q9M1V3; -.
DR ProteomicsDB; 249203; -.
DR EnsemblPlants; AT3G63370.1; AT3G63370.1; AT3G63370.
DR GeneID; 825512; -.
DR Gramene; AT3G63370.1; AT3G63370.1; AT3G63370.
DR KEGG; ath:AT3G63370; -.
DR Araport; AT3G63370; -.
DR TAIR; locus:2077279; AT3G63370.
DR eggNOG; KOG4197; Eukaryota.
DR HOGENOM; CLU_002706_15_6_1; -.
DR InParanoid; Q9M1V3; -.
DR OrthoDB; 1344243at2759; -.
DR PhylomeDB; Q9M1V3; -.
DR PRO; PR:Q9M1V3; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M1V3; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0031425; P:chloroplast RNA processing; IMP:TAIR.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0009451; P:RNA modification; IBA:GO_Central.
DR Gene3D; 1.25.40.10; -; 6.
DR InterPro; IPR032867; DYW_dom.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR Pfam; PF14432; DYW_deaminase; 1.
DR Pfam; PF01535; PPR; 9.
DR Pfam; PF13041; PPR_2; 2.
DR TIGRFAMs; TIGR00756; PPR; 6.
DR PROSITE; PS51375; PPR; 17.
PE 2: Evidence at transcript level;
KW 3D-structure; Chloroplast; mRNA processing; Plastid; Reference proteome;
KW Repeat; Transit peptide.
FT TRANSIT 1..64
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 65..960
FT /note="Pentatricopeptide repeat-containing protein
FT At3g63370, chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000356155"
FT REPEAT 79..109
FT /note="PPR 1"
FT REPEAT 115..145
FT /note="PPR 2"
FT REPEAT 146..180
FT /note="PPR 3"
FT REPEAT 181..215
FT /note="PPR 4"
FT REPEAT 216..246
FT /note="PPR 5"
FT REPEAT 248..282
FT /note="PPR 6"
FT REPEAT 283..317
FT /note="PPR 7"
FT REPEAT 319..349
FT /note="PPR 8"
FT REPEAT 350..384
FT /note="PPR 9"
FT REPEAT 385..419
FT /note="PPR 10"
FT REPEAT 420..450
FT /note="PPR 11"
FT REPEAT 451..485
FT /note="PPR 12"
FT REPEAT 486..516
FT /note="PPR 13"
FT REPEAT 520..550
FT /note="PPR 14"
FT REPEAT 551..585
FT /note="PPR 15"
FT REPEAT 586..620
FT /note="PPR 16"
FT REPEAT 621..651
FT /note="PPR 17"
FT REPEAT 652..686
FT /note="PPR 18"
FT REPEAT 687..717
FT /note="PPR 19"
FT REPEAT 723..753
FT /note="PPR 20"
FT REGION 758..833
FT /note="Type E motif"
FT REGION 834..864
FT /note="Type E(+) motif"
FT REGION 865..960
FT /note="Type DYW motif"
FT STRAND 828..832
FT /evidence="ECO:0007829|PDB:7O4F"
FT STRAND 835..839
FT /evidence="ECO:0007829|PDB:7O4F"
FT HELIX 850..867
FT /evidence="ECO:0007829|PDB:7O4F"
FT STRAND 872..878
FT /evidence="ECO:0007829|PDB:7O4F"
FT STRAND 881..883
FT /evidence="ECO:0007829|PDB:7O4F"
FT STRAND 885..888
FT /evidence="ECO:0007829|PDB:7O4F"
FT HELIX 893..904
FT /evidence="ECO:0007829|PDB:7O4F"
FT STRAND 911..917
FT /evidence="ECO:0007829|PDB:7O4F"
FT HELIX 921..933
FT /evidence="ECO:0007829|PDB:7O4F"
FT STRAND 938..941
FT /evidence="ECO:0007829|PDB:7O4F"
FT STRAND 946..950
FT /evidence="ECO:0007829|PDB:7O4F"
FT TURN 955..958
FT /evidence="ECO:0007829|PDB:7O4F"
SQ SEQUENCE 960 AA; 107520 MW; 135FEF513609DBB7 CRC64;
MEYAVTNMRL LSNMMYSASA ISFPRVRLHC SIPTEPSCRR NPFRQSNQPV QVPSPKLACF
DGVLTEAFQR LDVSENNSPV EAFAYVLELC GKRRAVSQGR QLHSRIFKTF PSFELDFLAG
KLVFMYGKCG SLDDAEKVFD EMPDRTAFAW NTMIGAYVSN GEPASALALY WNMRVEGVPL
GLSSFPALLK ACAKLRDIRS GSELHSLLVK LGYHSTGFIV NALVSMYAKN DDLSAARRLF
DGFQEKGDAV LWNSILSSYS TSGKSLETLE LFREMHMTGP APNSYTIVSA LTACDGFSYA
KLGKEIHASV LKSSTHSSEL YVCNALIAMY TRCGKMPQAE RILRQMNNAD VVTWNSLIKG
YVQNLMYKEA LEFFSDMIAA GHKSDEVSMT SIIAASGRLS NLLAGMELHA YVIKHGWDSN
LQVGNTLIDM YSKCNLTCYM GRAFLRMHDK DLISWTTVIA GYAQNDCHVE ALELFRDVAK
KRMEIDEMIL GSILRASSVL KSMLIVKEIH CHILRKGLLD TVIQNELVDV YGKCRNMGYA
TRVFESIKGK DVVSWTSMIS SSALNGNESE AVELFRRMVE TGLSADSVAL LCILSAAASL
SALNKGREIH CYLLRKGFCL EGSIAVAVVD MYACCGDLQS AKAVFDRIER KGLLQYTSMI
NAYGMHGCGK AAVELFDKMR HENVSPDHIS FLALLYACSH AGLLDEGRGF LKIMEHEYEL
EPWPEHYVCL VDMLGRANCV VEAFEFVKMM KTEPTAEVWC ALLAACRSHS EKEIGEIAAQ
RLLELEPKNP GNLVLVSNVF AEQGRWNDVE KVRAKMKASG MEKHPGCSWI EMDGKVHKFT
ARDKSHPESK EIYEKLSEVT RKLEREVGYV ADTKFVLHNV DEGEKVQMLH GHSERIAIAY
GLLRTPDRAC LRITKNLRVC RDCHTFCKLV SKLFRRDIVM RDANRFHHFE SGLCSCGDSW
