PP2B1_ARATH
ID PP2B1_ARATH Reviewed; 336 AA.
AC Q6NPT8; Q9ZVR6;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=F-box protein PP2-B1;
DE AltName: Full=Protein PHLOEM PROTEIN 2-LIKE B1;
DE Short=AtPP2-B1;
DE AltName: Full=SKP1-interacting partner 21;
GN Name=PP2B1; Synonyms=SKIP21; OrderedLocusNames=At2g02230;
GN ORFNames=T16F16.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INTERACTION WITH SKP1A/ASK1 AND SPK1B/ASK2.
RX PubMed=12795696; DOI=10.1046/j.1365-313x.2003.01768.x;
RA Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J.,
RA Hellmann H., Estelle M., Somers D.E., Crosby W.L.;
RT "Protein interaction analysis of SCF ubiquitin E3 ligase subunits from
RT Arabidopsis.";
RL Plant J. 34:753-767(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12529520; DOI=10.1104/pp.013086;
RA Dinant S., Clark A.M., Zhu Y., Vilaine F., Palauqui J.-C., Kusiak C.,
RA Thompson G.A.;
RT "Diversity of the superfamily of phloem lectins (phloem protein 2) in
RT angiosperms.";
RL Plant Physiol. 131:114-128(2003).
CC -!- FUNCTION: Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase
CC complexes, which may mediate the ubiquitination and subsequent
CC proteasomal degradation of target proteins. {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Part of a SCF (ASK-cullin-F-box) protein ligase complex (By
CC similarity). Interacts with SKP1A/ASK1 and SPK1B/ASK2. {ECO:0000250,
CC ECO:0000269|PubMed:12795696}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DOMAIN: The F-box is necessary for the interaction with ASK proteins.
CC {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC78508.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC005312; AAC78508.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC05559.1; -; Genomic_DNA.
DR EMBL; AK229118; BAF00994.1; -; mRNA.
DR EMBL; BT010729; AAR20786.1; -; mRNA.
DR EMBL; BT020602; AAW80875.1; -; mRNA.
DR PIR; E84434; E84434.
DR RefSeq; NP_178331.2; NM_126283.5.
DR AlphaFoldDB; Q6NPT8; -.
DR BioGRID; 157; 8.
DR IntAct; Q6NPT8; 2.
DR STRING; 3702.AT2G02230.1; -.
DR PaxDb; Q6NPT8; -.
DR PRIDE; Q6NPT8; -.
DR ProteomicsDB; 248969; -.
DR EnsemblPlants; AT2G02230.1; AT2G02230.1; AT2G02230.
DR GeneID; 814754; -.
DR Gramene; AT2G02230.1; AT2G02230.1; AT2G02230.
DR KEGG; ath:AT2G02230; -.
DR Araport; AT2G02230; -.
DR TAIR; locus:2056191; AT2G02230.
DR eggNOG; ENOG502QRA4; Eukaryota.
DR HOGENOM; CLU_050973_0_0_1; -.
DR InParanoid; Q6NPT8; -.
DR OMA; VWEMFLP; -.
DR OrthoDB; 1051457at2759; -.
DR PhylomeDB; Q6NPT8; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q6NPT8; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q6NPT8; baseline and differential.
DR Genevisible; Q6NPT8; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0030246; F:carbohydrate binding; ISS:TAIR.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR025886; PP2-like.
DR Pfam; PF00646; F-box; 1.
DR Pfam; PF14299; PP2; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
DR PROSITE; PS50181; FBOX; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..336
FT /note="F-box protein PP2-B1"
FT /id="PRO_0000272211"
FT DOMAIN 29..75
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 336 AA; 37130 MW; FB95AEAFCC5807C3 CRC64;
MEQIHGGDSN SGGGGGGSSR NDEISVTRAS RFDALPEDCI SKVISHTSPR DACVVASVSK
SVKSAAQSDL VWEMFLPSEY SSLVLQSANH LSKKEIFLSL ADNSVLVENG KKSFWVEKAS
GKKCYMLSAM ELTIIWGDSP AYWKWITVPE SKFEKVAELR NVCWFEVRGK ISCGMLSKGT
HYSVYVVFKT ANGRSYGFDL VPVEAGVGFV GKVATKKSVY FESGNADSRS ATSHYSGISY
AMVSRAFRMR RPWMQVQREE EEEVEGERER GMNVVGPKER VDGWSEVELG KFYINNGGCG
DDGSDEIEIS IMETQNGNWK SGLIIQGIEI RPERSN
