ATB14_ARATH
ID ATB14_ARATH Reviewed; 852 AA.
AC O04291;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Homeobox-leucine zipper protein ATHB-14;
DE AltName: Full=HD-ZIP protein ATHB-14;
DE AltName: Full=Homeodomain transcription factor ATHB-14;
DE AltName: Full=Protein PHABULOSA;
GN Name=ATHB-14; Synonyms=PHB; OrderedLocusNames=At2g34710; ORFNames=T29F13.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9747806; DOI=10.1023/a:1006016319613;
RA Sessa G., Steindler C., Morelli G., Ruberti I.;
RT "The Arabidopsis Athb-8, -9 and -14 genes are members of a small gene
RT family coding for highly related HD-ZIP proteins.";
RL Plant Mol. Biol. 38:609-622(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Ruzza V., Carabelli M., Ciarbelli A.R., Sessa G., Steindler C., Ruberti I.;
RT "Nucleotide sequence of the Arabidopsis ATHB-14 mRNA, encoding an HD-Zip
RT III protein related to ATHB-8.";
RL Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP FUNCTION, DEVELOPMENTAL STAGE, MUTAGENESIS OF GLY-202, AND TISSUE
RP SPECIFICITY.
RX PubMed=11395776; DOI=10.1038/35079635;
RA McConnell J.R., Emery J., Eshed Y., Bao N., Bowman J., Barton M.K.;
RT "Role of PHABULOSA and PHAVOLUTA in determining radial patterning in
RT shoots.";
RL Nature 411:709-713(2001).
RN [7]
RP INDUCTION.
RX PubMed=14999284; DOI=10.1038/nature02366;
RA Kidner C.A., Martienssen R.A.;
RT "Spatially restricted microRNA directs leaf polarity through ARGONAUTE1.";
RL Nature 428:81-84(2004).
RN [8]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=15328016; DOI=10.1016/j.ydbio.2004.05.037;
RA Sieber P., Gheyselinck J., Gross-Hardt R., Laux T., Grossniklaus U.,
RA Schneitz K.;
RT "Pattern formation during early ovule development in Arabidopsis
RT thaliana.";
RL Dev. Biol. 273:321-334(2004).
RN [9]
RP INDUCTION.
RX PubMed=16033795; DOI=10.1242/dev.01942;
RA Williams L., Grigg S.P., Xie M., Christensen S., Fletcher J.C.;
RT "Regulation of Arabidopsis shoot apical meristem and lateral organ
RT formation by microRNA miR166g and its AtHD-ZIP target genes.";
RL Development 132:3657-3668(2005).
RN [10]
RP FUNCTION.
RX PubMed=15598805; DOI=10.1105/tpc.104.026161;
RA Prigge M.J., Otsuga D., Alonso J.M., Ecker J.R., Drews G.N., Clark S.E.;
RT "Class III homeodomain-leucine zipper gene family members have overlapping,
RT antagonistic, and distinct roles in Arabidopsis development.";
RL Plant Cell 17:61-76(2005).
RN [11]
RP GENE FAMILY.
RX PubMed=16805899; DOI=10.1111/j.1525-142x.2006.00107.x;
RA Prigge M.J., Clark S.E.;
RT "Evolution of the class III HD-Zip gene family in land plants.";
RL Evol. Dev. 8:350-361(2006).
RN [12]
RP INTERACTION WITH ESR1 AND ESR2.
RX PubMed=17376809; DOI=10.1242/dev.001016;
RA Chandler J.W., Cole M., Flier A., Grewe B., Werr W.;
RT "The AP2 transcription factors DORNROSCHEN and DORNROSCHEN-LIKE redundantly
RT control Arabidopsis embryo patterning via interaction with PHAVOLUTA.";
RL Development 134:1653-1662(2007).
RN [13]
RP INDUCTION.
RX PubMed=17237362; DOI=10.1093/pcp/pcm008;
RA Zhou G.-K., Kubo M., Zhong R., Demura T., Ye Z.-H.;
RT "Overexpression of miR165 affects apical meristem formation, organ polarity
RT establishment and vascular development in Arabidopsis.";
RL Plant Cell Physiol. 48:391-404(2007).
RN [14]
RP INTERACTION WITH ZPR3, DOMAIN, AND ACTIVITY REGULATION.
RX PubMed=18408069; DOI=10.1105/tpc.107.057448;
RA Kim Y.S., Kim S.G., Lee M., Lee I., Park H.Y., Seo P.J., Jung J.H.,
RA Kwon E.J., Suh S.W., Paek K.H., Park C.M.;
RT "HD-ZIP III activity is modulated by competitive inhibitors via a feedback
RT loop in Arabidopsis shoot apical meristem development.";
RL Plant Cell 20:920-933(2008).
RN [15]
RP GENE FAMILY.
RX PubMed=25263420; DOI=10.1016/j.ympev.2014.06.017;
RA Floyd S.K., Ryan J.G., Conway S.J., Brenner E., Burris K.P., Burris J.N.,
RA Chen T., Edger P.P., Graham S.W., Leebens-Mack J.H., Pires J.C.,
RA Rothfels C.J., Sigel E.M., Stevenson D.W., Neal Stewart C. Jr., Wong G.K.,
RA Bowman J.L.;
RT "Origin of a novel regulatory module by duplication and degeneration of an
RT ancient plant transcription factor.";
RL Mol. Phylogenet. Evol. 81:159-173(2014).
CC -!- FUNCTION: Probable transcription factor involved in the determination
CC of adaxial-abaxial polarity in ovule primordium. Specifies adaxial leaf
CC fates. {ECO:0000269|PubMed:11395776, ECO:0000269|PubMed:15328016,
CC ECO:0000269|PubMed:15598805}.
CC -!- ACTIVITY REGULATION: Inhibited by ZPR3. {ECO:0000269|PubMed:18408069}.
CC -!- SUBUNIT: Homodimer (PubMed:18408069). Heterodimer with ZPR3
CC (PubMed:18408069). Interacts with ESR1 and ESR2 (PubMed:17376809).
CC Interacts with ZPR3 (PubMed:18408069). {ECO:0000269|PubMed:17376809,
CC ECO:0000269|PubMed:18408069}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in the center of the meristem and on the
CC adaxial side of the leaves. {ECO:0000269|PubMed:11395776}.
CC -!- DEVELOPMENTAL STAGE: Expressed at the youngest stages of ovule
CC development, predominantly to the adaxial side of the ovule primordium.
CC Later, expression becomes restricted to a region in the distal chalaza.
CC When the integuments initiate, expressed in the inner integument, with
CC highest expression in the inner layer of the inner integument.
CC Expressed in the vegetative shoot apical meristem (SAM) and initially
CC throughout the presumptive cotyledons. At the globular stage, just
CC before cotyledon outgrowth, expressed at high levels in cotyledon
CC adaxial domains. Expression is lost from the SAM of torpedo stage
CC embryos but is regained late in embryogenesis, extending to young
CC primordia after germination, and becoming progressively restricted to
CC the adaxial domain and the vasculature. Preferentially expressed in the
CC adaxial domain of the developing leaf. Expressed throughout the
CC plastochron 0 (P0) leaf primordium and expression increases in P1 to
CC become preferentially localized to the adaxial leaf domain by the P2
CC stage (polar expression). {ECO:0000269|PubMed:11395776,
CC ECO:0000269|PubMed:15328016}.
CC -!- INDUCTION: Repressed by miR165 and miR166.
CC {ECO:0000269|PubMed:14999284, ECO:0000269|PubMed:16033795,
CC ECO:0000269|PubMed:17237362}.
CC -!- DOMAIN: The ZIP domain (80-130) is necessary and sufficient for the
CC interaction with ZPR3 (PubMed:18408069). {ECO:0000269|PubMed:18408069}.
CC -!- SIMILARITY: Belongs to the HD-ZIP homeobox family. Class III subfamily.
CC {ECO:0000305}.
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DR EMBL; Y11122; CAA72007.1; -; Genomic_DNA.
DR EMBL; AJ441291; CAD29659.1; -; mRNA.
DR EMBL; AC003096; AAC16263.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09012.1; -; Genomic_DNA.
DR EMBL; AY099791; AAM20642.1; -; mRNA.
DR EMBL; BT000335; AAN15654.1; -; mRNA.
DR PIR; T01364; T01364.
DR RefSeq; NP_181018.1; NM_129025.4.
DR AlphaFoldDB; O04291; -.
DR BioGRID; 3382; 5.
DR IntAct; O04291; 3.
DR STRING; 3702.AT2G34710.1; -.
DR iPTMnet; O04291; -.
DR PaxDb; O04291; -.
DR PRIDE; O04291; -.
DR ProteomicsDB; 246717; -.
DR EnsemblPlants; AT2G34710.1; AT2G34710.1; AT2G34710.
DR GeneID; 818036; -.
DR Gramene; AT2G34710.1; AT2G34710.1; AT2G34710.
DR KEGG; ath:AT2G34710; -.
DR Araport; AT2G34710; -.
DR TAIR; locus:2061544; AT2G34710.
DR eggNOG; ENOG502QPKR; Eukaryota.
DR HOGENOM; CLU_012517_0_0_1; -.
DR InParanoid; O04291; -.
DR OMA; MCCSVKT; -.
DR OrthoDB; 146876at2759; -.
DR PhylomeDB; O04291; -.
DR PRO; PR:O04291; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O04291; baseline and differential.
DR Genevisible; O04291; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; ISS:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0008289; F:lipid binding; IEA:InterPro.
DR GO; GO:0009955; P:adaxial/abaxial pattern specification; IMP:TAIR.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0009855; P:determination of bilateral symmetry; IMP:TAIR.
DR GO; GO:0080060; P:integument development; IGI:TAIR.
DR GO; GO:0010014; P:meristem initiation; IMP:TAIR.
DR GO; GO:0009944; P:polarity specification of adaxial/abaxial axis; IMP:TAIR.
DR GO; GO:0010072; P:primary shoot apical meristem specification; IMP:TAIR.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 3.30.530.20; -; 1.
DR InterPro; IPR044830; HD-Zip_III.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR013978; MEKHLA.
DR InterPro; IPR023393; START-like_dom_sf.
DR InterPro; IPR002913; START_lipid-bd_dom.
DR PANTHER; PTHR45950; PTHR45950; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF08670; MEKHLA; 1.
DR Pfam; PF01852; START; 1.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00234; START; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS50848; START; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Differentiation; DNA-binding; Homeobox; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..852
FT /note="Homeobox-leucine zipper protein ATHB-14"
FT /id="PRO_0000331660"
FT DOMAIN 164..392
FT /note="START"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00197"
FT DNA_BIND 22..85
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 80..130
FT /note="ZIP domain"
FT /evidence="ECO:0000269|PubMed:18408069"
FT REGION 130..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 80..122
FT /evidence="ECO:0000255"
FT MUTAGEN 202
FT /note="G->D: In phb-3d, phb-4d and phb-5d; constitutively
FT active. Transformation of abaxial leaf fates into adaxial
FT leaf fates."
FT /evidence="ECO:0000269|PubMed:11395776"
SQ SEQUENCE 852 AA; 93218 MW; C1802529EFCC4E68 CRC64;
MMMVHSMSRD MMNRESPDKG LDSGKYVRYT PEQVEALERV YTECPKPSSL RRQQLIRECP
ILSNIEPKQI KVWFQNRRCR EKQRKEAARL QTVNRKLNAM NKLLMEENDR LQKQVSNLVY
ENGHMKHQLH TASGTTTDNS CESVVVSGQQ HQQQNPNPQH QQRDANNPAG LLSIAEEALA
EFLSKATGTA VDWVQMIGMK PGPDSIGIVA ISRNCSGIAA RACGLVSLEP MKVAEILKDR
PSWLRDCRSV DTLSVIPAGN GGTIELIYTQ MYAPTTLAAA RDFWTLRYST CLEDGSYVVC
ERSLTSATGG PTGPPSSNFV RAEMKPSGFL IRPCDGGGSI LHIVDHVDLD AWSVPEVMRP
LYESSKILAQ KMTVAALRHV RQIAQETSGE VQYGGGRQPA VLRTFSQRLC RGFNDAVNGF
VDDGWSPMGS DGAEDVTVMI NLSPGKFGGS QYGNSFLPSF GSGVLCAKAS MLLQNVPPAV
LVRFLREHRS EWADYGVDAY AAASLRASPF AVPCARAGGF PSNQVILPLA QTVEHEESLE
VVRLEGHAYS PEDMGLARDM YLLQLCSGVD ENVVGGCAQL VFAPIDESFA DDAPLLPSGF
RIIPLEQKST PNGASANRTL DLASALEGST RQAGEADPNG CNFRSVLTIA FQFTFDNHSR
DSVASMARQY VRSIVGSIQR VALAIAPRPG SNISPISVPT SPEALTLVRW ISRSYSLHTG
ADLFGSDSQT SGDTLLHQLW NHSDAILCCS LKTNASPVFT FANQTGLDML ETTLVALQDI
MLDKTLDEPG RKALCSEFPK IMQQGYAHLP AGVCASSMGR MVSYEQATVW KVLEDDESNH
CLAFMFVNWS FV
