PP308_ARATH
ID PP308_ARATH Reviewed; 612 AA.
AC O23266;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 3.
DT 25-MAY-2022, entry version 117.
DE RecName: Full=Pentatricopeptide repeat-containing protein At4g14050, mitochondrial;
DE AltName: Full=Mitochondrial editing factor 35 {ECO:0000303|PubMed:26470017};
DE Flags: Precursor;
GN Name=PCMP-H13; Synonyms=MEF35 {ECO:0000303|PubMed:26470017};
GN OrderedLocusNames=At4g14050; ORFNames=dl3066c, FCAALL.80;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9461215; DOI=10.1038/35140;
RA Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT thaliana.";
RL Nature 391:485-488(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-308.
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [5]
RP GENE FAMILY.
RX PubMed=10809006; DOI=10.1023/a:1006352315928;
RA Aubourg S., Boudet N., Kreis M., Lecharny A.;
RT "In Arabidopsis thaliana, 1% of the genome codes for a novel protein family
RT unique to plants.";
RL Plant Mol. Biol. 42:603-613(2000).
RN [6]
RP GENE FAMILY.
RX PubMed=15269332; DOI=10.1105/tpc.104.022236;
RA Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C.,
RA Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M.,
RA Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B.,
RA Taconnat L., Small I.;
RT "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins
RT reveals their essential role in organelle biogenesis.";
RL Plant Cell 16:2089-2103(2004).
RN [7]
RP FUNCTION, AND INTERACTION WITH MORF8/RIP1 AND MORF1/RIP8.
RX PubMed=26470017; DOI=10.1371/journal.pone.0140680;
RA Brehme N., Bayer-Csaszar E., Glass F., Takenaka M.;
RT "The DYW subgroup PPR protein MEF35 targets RNA editing sites in the
RT mitochondrial rpl16, nad4 and cob mRNAs in Arabidopsis thaliana.";
RL PLoS ONE 10:E0140680-E0140680(2015).
CC -!- FUNCTION: Involved in C-to-U editing of mitochondrial RNA. Required
CC specifically for editing the mitochondrial NAD4, MT-CYB/COB and RPL16
CC transcripts. {ECO:0000269|PubMed:26470017}.
CC -!- SUBUNIT: Interacts with MORF8/RIP1 and MORF1/RIP8.
CC {ECO:0000269|PubMed:26470017}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the PPR family. PCMP-H subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BX840233; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
CC Sequence=CAB46001.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB78447.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=Pentatricopeptide repeat proteins;
CC URL="https://ppr.plantenergy.uwa.edu.au";
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DR EMBL; Z97335; CAB46001.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161537; CAB78447.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE83365.1; -; Genomic_DNA.
DR EMBL; BX840233; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; B85153; B85153.
DR PIR; F71401; F71401.
DR RefSeq; NP_193141.2; NM_117480.4.
DR AlphaFoldDB; O23266; -.
DR SMR; O23266; -.
DR STRING; 3702.AT4G14050.1; -.
DR PaxDb; O23266; -.
DR PRIDE; O23266; -.
DR ProteomicsDB; 248980; -.
DR EnsemblPlants; AT4G14050.1; AT4G14050.1; AT4G14050.
DR GeneID; 827041; -.
DR Gramene; AT4G14050.1; AT4G14050.1; AT4G14050.
DR KEGG; ath:AT4G14050; -.
DR Araport; AT4G14050; -.
DR TAIR; locus:2129361; AT4G14050.
DR eggNOG; KOG4197; Eukaryota.
DR HOGENOM; CLU_002706_37_2_1; -.
DR InParanoid; O23266; -.
DR OMA; LSACKHH; -.
DR OrthoDB; 1344243at2759; -.
DR PhylomeDB; O23266; -.
DR PRO; PR:O23266; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O23266; baseline and differential.
DR Genevisible; O23266; AT.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0016554; P:cytidine to uridine editing; IMP:UniProtKB.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 1.25.40.10; -; 4.
DR InterPro; IPR032867; DYW_dom.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR Pfam; PF14432; DYW_deaminase; 1.
DR Pfam; PF01535; PPR; 5.
DR Pfam; PF13041; PPR_2; 1.
DR TIGRFAMs; TIGR00756; PPR; 5.
DR PROSITE; PS51375; PPR; 10.
PE 1: Evidence at protein level;
KW Mitochondrion; mRNA processing; Reference proteome; Repeat;
KW Transit peptide.
FT TRANSIT 1..24
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 25..612
FT /note="Pentatricopeptide repeat-containing protein
FT At4g14050, mitochondrial"
FT /id="PRO_0000363426"
FT REPEAT 37..71
FT /note="PPR 1"
FT REPEAT 72..103
FT /note="PPR 2"
FT REPEAT 104..138
FT /note="PPR 3"
FT REPEAT 139..169
FT /note="PPR 4"
FT REPEAT 170..204
FT /note="PPR 5"
FT REPEAT 205..235
FT /note="PPR 6"
FT REPEAT 237..271
FT /note="PPR 7"
FT REPEAT 272..302
FT /note="PPR 8"
FT REPEAT 303..337
FT /note="PPR 9"
FT REPEAT 338..373
FT /note="PPR 10"
FT REPEAT 374..408
FT /note="PPR 11"
FT REGION 409..485
FT /note="Type E motif"
FT REGION 486..516
FT /note="Type E(+) motif"
FT REGION 518..612
FT /note="Type DYW motif"
SQ SEQUENCE 612 AA; 68037 MW; AE0CB1C19017728F CRC64;
MLIPHYLHQL QLCARNRTLT TAKALHAHIV KLGIVQCCPL ANTLVNVYGK CGAASHALQV
FDEMPHRDHI AWASVLTALN QANLSGKTLS VFSSVGSSSG LRPDDFVFSA LVKACANLGS
IDHGRQVHCH FIVSEYANDE VVKSSLVDMY AKCGLLNSAK AVFDSIRVKN TISWTAMVSG
YAKSGRKEEA LELFRILPVK NLYSWTALIS GFVQSGKGLE AFSVFTEMRR ERVDILDPLV
LSSIVGACAN LAASIAGRQV HGLVIALGFD SCVFISNALI DMYAKCSDVI AAKDIFSRMR
HRDVVSWTSL IVGMAQHGQA EKALALYDDM VSHGVKPNEV TFVGLIYACS HVGFVEKGRE
LFQSMTKDYG IRPSLQHYTC LLDLLGRSGL LDEAENLIHT MPFPPDEPTW AALLSACKRQ
GRGQMGIRIA DHLVSSFKLK DPSTYILLSN IYASASLWGK VSEARRKLGE MEVRKDPGHS
SVEVRKETEV FYAGETSHPL KEDIFRLLKK LEEEMRIRNG YVPDTSWILH DMDEQEKEKL
LFWHSERSAV AYGLLKAVPG TPIRIVKNLR VCGDCHVVLK HISEITEREI IVRDATRYHH
FKGGKCSCND FW
