PP322_ARATH
ID PP322_ARATH Reviewed; 287 AA.
AC Q2V3H0; O49410; Q6NKP6;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 2.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Pentatricopeptide repeat-containing protein At4g18975, chloroplastic;
DE Flags: Precursor;
GN OrderedLocusNames=At4g18975; ORFNames=F13C5.140;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Carninci P., Hayashizaki Y.,
RA Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M.,
RA Shinozaki K., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY.
RX PubMed=15269332; DOI=10.1105/tpc.104.022236;
RA Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C.,
RA Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M.,
RA Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B.,
RA Taconnat L., Small I.;
RT "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins
RT reveals their essential role in organelle biogenesis.";
RL Plant Cell 16:2089-2103(2004).
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q2V3H0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q2V3H0-2; Sequence=VSP_036302;
CC Name=3;
CC IsoId=Q2V3H0-3; Sequence=VSP_036303;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform 3]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the PPR family. P subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA16754.1; Type=Erroneous gene model prediction; Note=The predicted gene has been split into 2 genes: At4g18970 and At4g18975.; Evidence={ECO:0000305};
CC Sequence=CAB78899.1; Type=Erroneous gene model prediction; Note=The predicted gene has been split into 2 genes: At4g18970 and At4g18975.; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=Pentatricopeptide repeat proteins;
CC URL="https://ppr.plantenergy.uwa.edu.au";
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DR EMBL; AL021711; CAA16754.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161549; CAB78899.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE84115.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE84116.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE84117.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE84118.1; -; Genomic_DNA.
DR EMBL; BT012648; AAT06467.1; -; mRNA.
DR EMBL; AK221620; BAD95227.1; -; mRNA.
DR PIR; T05034; T05034.
DR RefSeq; NP_001031667.1; NM_001036590.1. [Q2V3H0-2]
DR RefSeq; NP_001119009.1; NM_001125537.2. [Q2V3H0-1]
DR RefSeq; NP_001190768.1; NM_001203839.2. [Q2V3H0-1]
DR RefSeq; NP_567571.1; NM_118015.2. [Q2V3H0-1]
DR AlphaFoldDB; Q2V3H0; -.
DR SMR; Q2V3H0; -.
DR STRING; 3702.AT4G18975.4; -.
DR PaxDb; Q2V3H0; -.
DR PRIDE; Q2V3H0; -.
DR EnsemblPlants; AT4G18975.1; AT4G18975.1; AT4G18975. [Q2V3H0-1]
DR EnsemblPlants; AT4G18975.2; AT4G18975.2; AT4G18975. [Q2V3H0-2]
DR EnsemblPlants; AT4G18975.3; AT4G18975.3; AT4G18975. [Q2V3H0-1]
DR EnsemblPlants; AT4G18975.4; AT4G18975.4; AT4G18975. [Q2V3H0-1]
DR GeneID; 827633; -.
DR Gramene; AT4G18975.1; AT4G18975.1; AT4G18975. [Q2V3H0-1]
DR Gramene; AT4G18975.2; AT4G18975.2; AT4G18975. [Q2V3H0-2]
DR Gramene; AT4G18975.3; AT4G18975.3; AT4G18975. [Q2V3H0-1]
DR Gramene; AT4G18975.4; AT4G18975.4; AT4G18975. [Q2V3H0-1]
DR KEGG; ath:AT4G18975; -.
DR Araport; AT4G18975; -.
DR TAIR; locus:505006498; AT4G18975.
DR eggNOG; ENOG502QQWM; Eukaryota.
DR HOGENOM; CLU_049554_1_1_1; -.
DR InParanoid; Q2V3H0; -.
DR OMA; KWLRIIQ; -.
DR OrthoDB; 1214320at2759; -.
DR PhylomeDB; Q2V3H0; -.
DR PRO; PR:Q2V3H0; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q2V3H0; baseline and differential.
DR Genevisible; Q2V3H0; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR044646; EMB1417-like.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR46782; PTHR46782; 1.
DR TIGRFAMs; TIGR00756; PPR; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chloroplast; Plastid; Reference proteome; Repeat;
KW Transit peptide.
FT TRANSIT 1..34
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 35..287
FT /note="Pentatricopeptide repeat-containing protein
FT At4g18975, chloroplastic"
FT /id="PRO_0000363439"
FT REPEAT 165..199
FT /note="PPR 1"
FT REPEAT 201..235
FT /note="PPR 2"
FT VAR_SEQ 1..55
FT /note="MALCNLNPTQGIFPLQGLSKSQEFICFSLLQSPRCGSYSSLKTKRFGFCIRS
FT KFS -> MLFFASISTLRKLLLVEDEKVWLLHQIK (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_036302"
FT VAR_SEQ 153..287
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|Ref.3, ECO:0000303|Ref.4"
FT /id="VSP_036303"
SQ SEQUENCE 287 AA; 33252 MW; 6C8BAEB13808626F CRC64;
MALCNLNPTQ GIFPLQGLSK SQEFICFSLL QSPRCGSYSS LKTKRFGFCI RSKFSEKEAG
KLDRGYVATV NSKEIKKVGK KEHHLWKKND SAGSGQKALN LVRMLSGLPN EKEAVYGALN
KWVAWEVEFP IIAAAKALQI LRKRSQWHRV IQLAKWMLSK GQGATMGTYD ILLLAFDMDE
RADEAESLWN MILHTHTRSI PRRLFARMIA LYAHHDLHDK VIEVFADMEE LKVSPDEDSA
RRVARAFREL NQEENRKLIL RRYLSEYKYI YFNGERVRVK RYFSEDS
