PP344_ARATH
ID PP344_ARATH Reviewed; 1112 AA.
AC Q9SZ52;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 115.
DE RecName: Full=Pentatricopeptide repeat-containing protein At4g31850, chloroplastic;
DE AltName: Full=Protein PROTON GRADIENT REGULATION 3;
DE Flags: Precursor;
GN Name=PGR3; OrderedLocusNames=At4g31850; ORFNames=F11C18.50;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=15269332; DOI=10.1105/tpc.104.022236;
RA Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C.,
RA Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M.,
RA Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B.,
RA Taconnat L., Small I.;
RT "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins
RT reveals their essential role in organelle biogenesis.";
RL Plant Cell 16:2089-2103(2004).
RN [4]
RP FUNCTION, AND MUTAGENESIS OF THR-505 AND THR-610.
RX PubMed=15053768; DOI=10.1111/j.1365-313x.2004.02035.x;
RA Yamazaki H., Tasaka M., Shikanai T.;
RT "PPR motifs of the nucleus-encoded factor, PGR3, function in the selective
RT and distinct steps of chloroplast gene expression in Arabidopsis.";
RL Plant J. 38:152-163(2004).
CC -!- FUNCTION: Plays a role in the stabilization of the primary
CC polycistronic transcript of the petL operon encoding subunits of the
CC cytochrome b6-f complex. {ECO:0000269|PubMed:15053768}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the PPR family. P subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Pentatricopeptide repeat proteins;
CC URL="https://ppr.plantenergy.uwa.edu.au";
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DR EMBL; AL049607; CAB40755.1; -; Genomic_DNA.
DR EMBL; AL161579; CAB79903.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85967.1; -; Genomic_DNA.
DR PIR; T06307; T06307.
DR RefSeq; NP_194913.1; NM_119335.2.
DR AlphaFoldDB; Q9SZ52; -.
DR SMR; Q9SZ52; -.
DR STRING; 3702.AT4G31850.1; -.
DR PaxDb; Q9SZ52; -.
DR PRIDE; Q9SZ52; -.
DR ProteomicsDB; 248993; -.
DR EnsemblPlants; AT4G31850.1; AT4G31850.1; AT4G31850.
DR GeneID; 829314; -.
DR Gramene; AT4G31850.1; AT4G31850.1; AT4G31850.
DR KEGG; ath:AT4G31850; -.
DR Araport; AT4G31850; -.
DR TAIR; locus:2116772; AT4G31850.
DR eggNOG; KOG4197; Eukaryota.
DR HOGENOM; CLU_002706_49_3_1; -.
DR InParanoid; Q9SZ52; -.
DR OMA; VQPTAMY; -.
DR OrthoDB; 1344243at2759; -.
DR PhylomeDB; Q9SZ52; -.
DR PRO; PR:Q9SZ52; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SZ52; baseline and differential.
DR Genevisible; Q9SZ52; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0032544; P:plastid translation; IMP:TAIR.
DR GO; GO:0043489; P:RNA stabilization; IMP:TAIR.
DR GO; GO:0006412; P:translation; IMP:TAIR.
DR Gene3D; 1.25.40.10; -; 9.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR033443; PPR_long.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR Pfam; PF01535; PPR; 3.
DR Pfam; PF12854; PPR_1; 2.
DR Pfam; PF13041; PPR_2; 8.
DR Pfam; PF13812; PPR_3; 1.
DR Pfam; PF17177; PPR_long; 1.
DR TIGRFAMs; TIGR00756; PPR; 22.
DR PROSITE; PS51375; PPR; 27.
PE 1: Evidence at protein level;
KW Chloroplast; Plastid; Reference proteome; Repeat; Transit peptide.
FT TRANSIT 1..62
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 63..1112
FT /note="Pentatricopeptide repeat-containing protein
FT At4g31850, chloroplastic"
FT /id="PRO_0000363461"
FT REPEAT 117..151
FT /note="PPR 1"
FT REPEAT 152..186
FT /note="PPR 2"
FT REPEAT 187..221
FT /note="PPR 3"
FT REPEAT 222..256
FT /note="PPR 4"
FT REPEAT 257..291
FT /note="PPR 5"
FT REPEAT 292..326
FT /note="PPR 6"
FT REPEAT 327..361
FT /note="PPR 7"
FT REPEAT 362..396
FT /note="PPR 8"
FT REPEAT 397..431
FT /note="PPR 9"
FT REPEAT 432..466
FT /note="PPR 10"
FT REPEAT 467..501
FT /note="PPR 11"
FT REPEAT 502..536
FT /note="PPR 12"
FT REPEAT 537..571
FT /note="PPR 13"
FT REPEAT 572..606
FT /note="PPR 14"
FT REPEAT 607..641
FT /note="PPR 15"
FT REPEAT 642..672
FT /note="PPR 16"
FT REPEAT 676..711
FT /note="PPR 17"
FT REPEAT 712..746
FT /note="PPR 18"
FT REPEAT 748..783
FT /note="PPR 19"
FT REPEAT 784..818
FT /note="PPR 20"
FT REPEAT 819..853
FT /note="PPR 21"
FT REPEAT 854..889
FT /note="PPR 22"
FT REPEAT 890..924
FT /note="PPR 23"
FT REPEAT 925..959
FT /note="PPR 24"
FT REPEAT 960..994
FT /note="PPR 25"
FT REPEAT 995..1030
FT /note="PPR 26"
FT REPEAT 1031..1065
FT /note="PPR 27"
FT REPEAT 1066..1100
FT /note="PPR 28"
FT MUTAGEN 505
FT /note="T->I: In pgr3-2; Affects the accumulation of the
FT petL polycistronic RNA."
FT /evidence="ECO:0000269|PubMed:15053768"
FT MUTAGEN 610
FT /note="T->I: In pgr3-1; Affects the accumulation of the
FT petL polycistronic RNA."
FT /evidence="ECO:0000269|PubMed:15053768"
SQ SEQUENCE 1112 AA; 124194 MW; 0F174AD6856331C4 CRC64;
MVALLCSASL CGDISVGDAC LVSNIKAKCR DNLVTGGLKF HALKIGSRKK HWRRKSMRCS
VVSMKSSDFS GSMIRKSSKP DLSSSEEVTR GLKSFPDTDS SFSYFKSVAG NLNLVHTTET
CNYMLEALRV DGKLEEMAYV FDLMQKRIIK RDTNTYLTIF KSLSVKGGLK QAPYALRKMR
EFGFVLNAYS YNGLIHLLLK SRFCTEAMEV YRRMILEGFR PSLQTYSSLM VGLGKRRDID
SVMGLLKEME TLGLKPNVYT FTICIRVLGR AGKINEAYEI LKRMDDEGCG PDVVTYTVLI
DALCTARKLD CAKEVFEKMK TGRHKPDRVT YITLLDRFSD NRDLDSVKQF WSEMEKDGHV
PDVVTFTILV DALCKAGNFG EAFDTLDVMR DQGILPNLHT YNTLICGLLR VHRLDDALEL
FGNMESLGVK PTAYTYIVFI DYYGKSGDSV SALETFEKMK TKGIAPNIVA CNASLYSLAK
AGRDREAKQI FYGLKDIGLV PDSVTYNMMM KCYSKVGEID EAIKLLSEMM ENGCEPDVIV
VNSLINTLYK ADRVDEAWKM FMRMKEMKLK PTVVTYNTLL AGLGKNGKIQ EAIELFEGMV
QKGCPPNTIT FNTLFDCLCK NDEVTLALKM LFKMMDMGCV PDVFTYNTII FGLVKNGQVK
EAMCFFHQMK KLVYPDFVTL CTLLPGVVKA SLIEDAYKII TNFLYNCADQ PANLFWEDLI
GSILAEAGID NAVSFSERLV ANGICRDGDS ILVPIIRYSC KHNNVSGART LFEKFTKDLG
VQPKLPTYNL LIGGLLEADM IEIAQDVFLQ VKSTGCIPDV ATYNFLLDAY GKSGKIDELF
ELYKEMSTHE CEANTITHNI VISGLVKAGN VDDALDLYYD LMSDRDFSPT ACTYGPLIDG
LSKSGRLYEA KQLFEGMLDY GCRPNCAIYN ILINGFGKAG EADAACALFK RMVKEGVRPD
LKTYSVLVDC LCMVGRVDEG LHYFKELKES GLNPDVVCYN LIINGLGKSH RLEEALVLFN
EMKTSRGITP DLYTYNSLIL NLGIAGMVEE AGKIYNEIQR AGLEPNVFTF NALIRGYSLS
GKPEHAYAVY QTMVTGGFSP NTGTYEQLPN RA
