PP346_ARATH
ID PP346_ARATH Reviewed; 537 AA.
AC Q9SUU7; Q680T6;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Pentatricopeptide repeat-containing protein At4g32450, mitochondrial;
DE Flags: Precursor;
GN Name=PCMP-H63; OrderedLocusNames=At4g32450; ORFNames=F8B4.150;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY.
RX PubMed=10809006; DOI=10.1023/a:1006352315928;
RA Aubourg S., Boudet N., Kreis M., Lecharny A.;
RT "In Arabidopsis thaliana, 1% of the genome codes for a novel protein family
RT unique to plants.";
RL Plant Mol. Biol. 42:603-613(2000).
RN [6]
RP GENE FAMILY.
RX PubMed=15269332; DOI=10.1105/tpc.104.022236;
RA Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C.,
RA Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M.,
RA Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B.,
RA Taconnat L., Small I.;
RT "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins
RT reveals their essential role in organelle biogenesis.";
RL Plant Cell 16:2089-2103(2004).
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the PPR family. PCMP-H subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Pentatricopeptide repeat proteins;
CC URL="https://ppr.plantenergy.uwa.edu.au";
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DR EMBL; AL034567; CAA22572.1; -; Genomic_DNA.
DR EMBL; AL161581; CAB79962.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86062.1; -; Genomic_DNA.
DR EMBL; BT015164; AAT85760.1; -; mRNA.
DR EMBL; AK175781; BAD43544.1; -; mRNA.
DR PIR; T05355; T05355.
DR RefSeq; NP_194971.1; NM_119397.2.
DR AlphaFoldDB; Q9SUU7; -.
DR SMR; Q9SUU7; -.
DR BioGRID; 14666; 1.
DR IntAct; Q9SUU7; 1.
DR STRING; 3702.AT4G32450.1; -.
DR PaxDb; Q9SUU7; -.
DR PRIDE; Q9SUU7; -.
DR ProteomicsDB; 248994; -.
DR EnsemblPlants; AT4G32450.1; AT4G32450.1; AT4G32450.
DR GeneID; 829380; -.
DR Gramene; AT4G32450.1; AT4G32450.1; AT4G32450.
DR KEGG; ath:AT4G32450; -.
DR Araport; AT4G32450; -.
DR TAIR; locus:2127826; AT4G32450.
DR eggNOG; KOG4197; Eukaryota.
DR HOGENOM; CLU_002706_45_0_1; -.
DR InParanoid; Q9SUU7; -.
DR OMA; KSWRNEG; -.
DR OrthoDB; 808695at2759; -.
DR PhylomeDB; Q9SUU7; -.
DR PRO; PR:Q9SUU7; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SUU7; baseline and differential.
DR Genevisible; Q9SUU7; AT.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0009451; P:RNA modification; IBA:GO_Central.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR032867; DYW_dom.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR045215; PPR_prot_plant.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR24015; PTHR24015; 1.
DR Pfam; PF14432; DYW_deaminase; 1.
DR Pfam; PF01535; PPR; 2.
DR TIGRFAMs; TIGR00756; PPR; 2.
DR PROSITE; PS51375; PPR; 5.
PE 2: Evidence at transcript level;
KW Mitochondrion; Reference proteome; Repeat; Transit peptide.
FT TRANSIT 1..110
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 111..537
FT /note="Pentatricopeptide repeat-containing protein
FT At4g32450, mitochondrial"
FT /id="PRO_0000363463"
FT REPEAT 145..179
FT /note="PPR 1"
FT REPEAT 180..210
FT /note="PPR 2"
FT REPEAT 215..249
FT /note="PPR 3"
FT REPEAT 250..280
FT /note="PPR 4"
FT REPEAT 281..316
FT /note="PPR 5"
FT REPEAT 317..347
FT /note="PPR 6"
FT REGION 113..148
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 412..442
FT /note="Type E(+) motif"
FT REGION 443..537
FT /note="Type DYW motif"
FT COMPBIAS 113..144
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 187
FT /note="I -> M (in Ref. 4; BAD43544)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 537 AA; 60537 MW; 266F6DE51AD4E472 CRC64;
MIYTLTRGSL LGSTCKLRYS SLFSYLSTAA LRLGFENPTN GNPMDNSSHH IGYVNGFNGG
EQSLGGFQQN SYEQSLNPVS GQNPTNRFYQ NGYNRNQSYG EHSEIINQRN QNWQSSDGCS
SYGTTGNGVP QENNTGGNHF QQDHSGHSSL DELDSICREG KVKKAVEIIK SWRNEGYVVD
LPRLFWIAQL CGDAQALQEA KVVHEFITSS VGISDISAYN SIIEMYSGCG SVEDALTVFN
SMPERNLETW CGVIRCFAKN GQGEDAIDTF SRFKQEGNKP DGEMFKEIFF ACGVLGDMNE
GLLHFESMYK EYGIIPCMEH YVSLVKMLAE PGYLDEALRF VESMEPNVDL WETLMNLSRV
HGDLILGDRC QDMVEQLDAS RLNKESKAGL VPVKSSDLVK EKLQRMAKGP NYGIRYMAAG
DISRPENREL YMALKSLKEH MIEIGYVPLS KLALHDVDQE SKDENLFNHN ERFAFISTFL
DTPARSLIRV MKNLRVCADC HNALKLMSKI VGRELISRDA KRFHHMKDGV CSCREYW
