PP349_ARATH
ID PP349_ARATH Reviewed; 1089 AA.
AC Q0WLC6; Q0WVK9; Q6NLP9; Q9SW51; Q9SW52;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 2.
DT 25-MAY-2022, entry version 91.
DE RecName: Full=Pentatricopeptide repeat-containing protein MRL1, chloroplastic;
DE AltName: Full=Protein MATURATION OF RBCL 1;
DE Short=AtMRL1;
DE Flags: Precursor;
GN Name=MRL1; OrderedLocusNames=At4g34830/At4g34820;
GN ORFNames=T11I11.70/T11I11.60;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 769-1089.
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Carninci P., Hayashizaki Y.,
RA Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M.,
RA Shinozaki K., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY.
RX PubMed=15269332; DOI=10.1105/tpc.104.022236;
RA Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C.,
RA Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M.,
RA Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B.,
RA Taconnat L., Small I.;
RT "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins
RT reveals their essential role in organelle biogenesis.";
RL Plant Cell 16:2089-2103(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=18431481; DOI=10.1371/journal.pone.0001994;
RA Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q.,
RA van Wijk K.J.;
RT "Sorting signals, N-terminal modifications and abundance of the chloroplast
RT proteome.";
RL PLoS ONE 3:E1994-E1994(2008).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=20097872; DOI=10.1105/tpc.109.066266;
RA Johnson X., Wostrikoff K., Finazzi G., Kuras R., Schwarz C., Bujaldon S.,
RA Nickelsen J., Stern D.B., Wollman F.A., Vallon O.;
RT "MRL1, a conserved Pentatricopeptide repeat protein, is required for
RT stabilization of rbcL mRNA in Chlamydomonas and Arabidopsis.";
RL Plant Cell 22:234-248(2010).
CC -!- FUNCTION: Regulator of the large subunit (LS) of RuBisCO. Involved
CC either in the processing or in the stabilization of the processed
CC transcript, probably by acting as a barrier to the 5'>3' degradation.
CC {ECO:0000269|PubMed:20097872}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:18431481}.
CC -!- TISSUE SPECIFICITY: Expressed in stems, leaves and sepals.
CC {ECO:0000269|PubMed:20097872}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. RuBisCO content only
CC slightly reduced. {ECO:0000269|PubMed:20097872}.
CC -!- SIMILARITY: Belongs to the PPR family. P subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB45443.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes At4g34820 and At4g34830.; Evidence={ECO:0000305};
CC Sequence=CAB45444.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes At4g34820 and At4g34830.; Evidence={ECO:0000305};
CC Sequence=CAB80199.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes At4g34820 and At4g34830.; Evidence={ECO:0000305};
CC Sequence=CAB80200.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes At4g34820 and At4g34830.; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=Pentatricopeptide repeat proteins;
CC URL="https://ppr.plantenergy.uwa.edu.au";
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL079347; CAB45443.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL079347; CAB45444.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161586; CAB80199.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161586; CAB80200.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE86426.1; -; Genomic_DNA.
DR EMBL; AK226737; BAE98839.1; -; mRNA.
DR EMBL; AK230279; BAF02081.1; -; mRNA.
DR EMBL; BT012281; AAS76768.1; -; mRNA.
DR PIR; T10228; T10228.
DR PIR; T10229; T10229.
DR RefSeq; NP_195209.2; NM_119649.5.
DR AlphaFoldDB; Q0WLC6; -.
DR SMR; Q0WLC6; -.
DR STRING; 3702.AT4G34830.1; -.
DR iPTMnet; Q0WLC6; -.
DR PaxDb; Q0WLC6; -.
DR PRIDE; Q0WLC6; -.
DR ProteomicsDB; 249243; -.
DR EnsemblPlants; AT4G34830.1; AT4G34830.1; AT4G34830.
DR GeneID; 829635; -.
DR Gramene; AT4G34830.1; AT4G34830.1; AT4G34830.
DR KEGG; ath:AT4G34830; -.
DR Araport; AT4G34830; -.
DR TAIR; locus:2116915; AT4G34830.
DR eggNOG; KOG4197; Eukaryota.
DR HOGENOM; CLU_009360_0_0_1; -.
DR InParanoid; Q0WLC6; -.
DR OMA; EVYKMIH; -.
DR OrthoDB; 1344243at2759; -.
DR PhylomeDB; Q0WLC6; -.
DR PRO; PR:Q0WLC6; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q0WLC6; baseline and differential.
DR Genevisible; Q0WLC6; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0048255; P:mRNA stabilization; IMP:TAIR.
DR Gene3D; 1.25.40.10; -; 4.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR033443; PPR_long.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR Pfam; PF17177; PPR_long; 2.
DR TIGRFAMs; TIGR00756; PPR; 8.
DR PROSITE; PS51375; PPR; 10.
PE 1: Evidence at protein level;
KW Chloroplast; Plastid; Reference proteome; Repeat; Transit peptide.
FT TRANSIT 1..72
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 73..1089
FT /note="Pentatricopeptide repeat-containing protein MRL1,
FT chloroplastic"
FT /id="PRO_0000363466"
FT REPEAT 466..500
FT /note="PPR 1"
FT REPEAT 501..535
FT /note="PPR 2"
FT REPEAT 536..570
FT /note="PPR 3"
FT REPEAT 571..605
FT /note="PPR 4"
FT REPEAT 608..642
FT /note="PPR 5"
FT REPEAT 643..677
FT /note="PPR 6"
FT REPEAT 678..712
FT /note="PPR 7"
FT REPEAT 713..747
FT /note="PPR 8"
FT REPEAT 748..782
FT /note="PPR 9"
FT REPEAT 783..817
FT /note="PPR 10"
FT CONFLICT 163
FT /note="E -> G (in Ref. 3; BAE98839)"
FT /evidence="ECO:0000305"
FT CONFLICT 195
FT /note="D -> G (in Ref. 3; BAF02081)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1089 AA; 119771 MW; 4B98B4F07A964511 CRC64;
MEVTSTTFIS TTRSSKYLTL TSYSPVILPA STLRRDFLGC CHSLRPSPHL RTRAGKRNSR
RSSIRSPRLV VRASIDSGLI LIVVAVTAFS AIAFAYCQST FRKRKSSDEV ATVHGGKNSA
ENRREIHGDI HEGNPVEINV GFRKVEEESV NLLEEEKAHQ IHEVAVMDYD SVSAEDSQFA
VASVTTVATA HTLIDESFSS SIVNGSVALE SATFGVKTPE KQVGNSEDQK GLEHDFSQAV
VGIHSIASPQ VVDDTRALEY EYNGLLQKPL EYSIFAESKR EEIHTFYGSN HSSAKSSRLP
SLKAVSPAVT SATNSLFLDH KNNGVIDTQF PGQSSGQATG DVQEENLVAH SNGGVSHIRK
DVKGDWKFPS DGKHVGHQID ESMPQFPARN FELHNSNGRS PETSDAYNRL LRDGRIKDCI
SLLEDLDQRD LLDMDKIYHA SFFKACKKQR AVKEAFRFTK LILNPTMSTF NMLMSVCASS
QDIEGARGVL RLVQESGMTA DCKLYTTLIS SCAKSGKVDA MFEVFHQMSN SGVEANLHTF
GALIDGCARA GQVAKAFGAY GILRSKNVKP DRVVFNALIS ACGQSGAVDR AFDVLAEMKA
ETHPIDPDHI SIGALMKACC NAGQVERAKE VYQMIHKYGI RGTPEVYTIA VNSCSKSGDW
DFACSIYKDM KEKDVTPDEV FFSALIDVAG HAKMLDEAFG ILQDAKSQGI RLGTISYSSL
MGACCNAKDW KKALELYEKI KSIKLRPTIS TMNALITALC EGNQLPKAME YLDEIKTLGL
KPNTITYSML MLASERKDDF EVSFKLLSQA KGDGVSPNLI MCRCITSLCK RRFEKACAGG
EPVVSFKSGR PQIENKWTSM ALMVYRETIS GGTVPTTEVV SQVLGCLQLP HDAALRDRLI
STLGINISSQ KQHNIFPLVD GFGEYDPRAF SLLEEATSLG VLPSVSFNKI PLFFDTTELP
KNVAEVYLLT IFKGLKHRLA AGAKIPHINL IISIQEKEIR TPEGEKTIDL TGRVGQEIGA
LLRRLDIPYH RKDSRLRING VSLKNWFQPK LDSPFSGGKP GDLRSSQVPL GNQISRQQRS
IRLGNLSLE
