ID   PP351_ARATH             Reviewed;         444 AA.
AC   Q8VYR5; Q8L9P6; Q9SZT3;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Pentatricopeptide repeat-containing protein At4g35850, mitochondrial;
DE   Flags: Precursor;
GN   OrderedLocusNames=At4g35850; ORFNames=F4B14_120;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA   Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA   Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA   de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA   Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA   Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA   Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA   Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA   Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA   Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA   Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA   Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA   Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA   Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA   Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA   Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA   Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA   Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA   Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA   Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA   Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA   Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA   Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA   de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA   Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA   Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA   Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA   Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA   Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA   Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA   Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA   O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA   Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA   Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL   Nature 402:769-777(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP   ANALYSIS].
RC   STRAIN=cv. Landsberg erecta;
RX   PubMed=14671022; DOI=10.1105/tpc.016055;
RA   Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J.,
RA   Millar A.H.;
RT   "Experimental analysis of the Arabidopsis mitochondrial proteome highlights
RT   signaling and regulatory components, provides assessment of targeting
RT   prediction programs, and indicates plant-specific mitochondrial proteins.";
RL   Plant Cell 16:241-256(2004).
RN   [6]
RP   GENE FAMILY.
RX   PubMed=15269332; DOI=10.1105/tpc.104.022236;
RA   Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C.,
RA   Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M.,
RA   Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B.,
RA   Taconnat L., Small I.;
RT   "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins
RT   reveals their essential role in organelle biogenesis.";
RL   Plant Cell 16:2089-2103(2004).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND CLEAVAGE OF TRANSIT PEPTIDE AFTER
RP   PHE-25.
RX   PubMed=25732537; DOI=10.1093/jxb/erv064;
RA   Carrie C., Venne A.S., Zahedi R.P., Soll J.;
RT   "Identification of cleavage sites and substrate proteins for two
RT   mitochondrial intermediate peptidases in Arabidopsis thaliana.";
RL   J. Exp. Bot. 66:2691-2708(2015).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14671022,
CC       ECO:0000305|PubMed:25732537}.
CC   -!- SIMILARITY: Belongs to the PPR family. P subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA21471.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=CAB81494.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC   -!- WEB RESOURCE: Name=Pentatricopeptide repeat proteins;
CC       URL="https://ppr.plantenergy.uwa.edu.au";
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DR   EMBL; AL031986; CAA21471.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL161588; CAB81494.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002687; AEE86580.1; -; Genomic_DNA.
DR   EMBL; AY070070; AAL49827.1; -; mRNA.
DR   EMBL; AY091334; AAM14273.1; -; mRNA.
DR   EMBL; AY088310; AAM65849.1; -; mRNA.
DR   PIR; T04695; T04695.
DR   RefSeq; NP_567990.1; NM_119751.3.
DR   AlphaFoldDB; Q8VYR5; -.
DR   SMR; Q8VYR5; -.
DR   BioGRID; 15021; 3.
DR   IntAct; Q8VYR5; 2.
DR   STRING; 3702.AT4G35850.1; -.
DR   PaxDb; Q8VYR5; -.
DR   PRIDE; Q8VYR5; -.
DR   ProteomicsDB; 249245; -.
DR   EnsemblPlants; AT4G35850.1; AT4G35850.1; AT4G35850.
DR   GeneID; 829739; -.
DR   Gramene; AT4G35850.1; AT4G35850.1; AT4G35850.
DR   KEGG; ath:AT4G35850; -.
DR   Araport; AT4G35850; -.
DR   TAIR; locus:2125379; AT4G35850.
DR   eggNOG; KOG4197; Eukaryota.
DR   HOGENOM; CLU_038751_0_0_1; -.
DR   InParanoid; Q8VYR5; -.
DR   OMA; GHTPRGM; -.
DR   OrthoDB; 1344243at2759; -.
DR   PhylomeDB; Q8VYR5; -.
DR   PRO; PR:Q8VYR5; -.
DR   Proteomes; UP000006548; Chromosome 4.
DR   ExpressionAtlas; Q8VYR5; baseline and differential.
DR   Genevisible; Q8VYR5; AT.
DR   GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR   GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR   Gene3D; 1.25.40.10; -; 2.
DR   InterPro; IPR002885; Pentatricopeptide_repeat.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   Pfam; PF13041; PPR_2; 1.
DR   TIGRFAMs; TIGR00756; PPR; 1.
DR   PROSITE; PS51375; PPR; 7.
PE   1: Evidence at protein level;
KW   Mitochondrion; Reference proteome; Repeat; Transit peptide.
FT   TRANSIT         1..25
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000269|PubMed:25732537"
FT   CHAIN           26..444
FT                   /note="Pentatricopeptide repeat-containing protein
FT                   At4g35850, mitochondrial"
FT                   /id="PRO_0000363468"
FT   REPEAT          40..74
FT                   /note="PPR 1"
FT   REPEAT          75..109
FT                   /note="PPR 2"
FT   REPEAT          110..144
FT                   /note="PPR 3"
FT   REPEAT          145..179
FT                   /note="PPR 4"
FT   REPEAT          255..289
FT                   /note="PPR 5"
FT   REPEAT          290..325
FT                   /note="PPR 6"
FT   CONFLICT        294
FT                   /note="M -> V (in Ref. 4; AAM65849)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   444 AA;  50457 MW;  CB50891E2908C765 CRC64;
     MKFLMQSISG RNRSLVRALV SRRYFASSPE EIAKRNYAND LSEYNTAVNS VTAQRRHYLL
     RDVYDDMKLD GVQPTADIFH SFVVGTMKGA RLSDAFFFRE EMKAMGIAPD VNLYNFLIST
     CGKCKNGKEA IRVYDEMKRY DVKPNGQTFV CLLNACAVSG QLDLVYAIVR DMTAAGVGLN
     QFCYAGLITA HLNKQPRPDN LSTKILEFVE QSKGWSAIDS SRKSAEDVMF SISEEELYNI
     PTADYSHRTR FLQRNLTVYH VAFSALADLK DVKATEALLE MLKKDGKDTD TYCMLQIMRC
     YLHSQDFENG LKLFQDYMSA DKIPAMELYT TLIEGAMTGY TDNGMKIAQD TLIQMNERNF
     FLDPRTGSNL LLKAAGEKTG GYTVANMIWD LMLARNILPT LAAVEAYYKG LKEREIPEDD
     PRLMLVTRTY NNLRLREGTL PNRR