PP354_ARATH
ID PP354_ARATH Reviewed; 632 AA.
AC Q9SZT8;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Pentatricopeptide repeat-containing protein ELI1, chloroplastic {ECO:0000305};
DE AltName: Full=Protein EDITING LACKING INSERTIONAL MUTANT 1 {ECO:0000303|PubMed:24194514};
DE Flags: Precursor;
GN Name=ELI1 {ECO:0000303|PubMed:24194514}; Synonyms=PCMP-H48;
GN OrderedLocusNames=At4g37380; ORFNames=F6G17.30;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=10809006; DOI=10.1023/a:1006352315928;
RA Aubourg S., Boudet N., Kreis M., Lecharny A.;
RT "In Arabidopsis thaliana, 1% of the genome codes for a novel protein family
RT unique to plants.";
RL Plant Mol. Biol. 42:603-613(2000).
RN [4]
RP GENE FAMILY.
RX PubMed=15269332; DOI=10.1105/tpc.104.022236;
RA Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C.,
RA Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M.,
RA Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B.,
RA Taconnat L., Small I.;
RT "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins
RT reveals their essential role in organelle biogenesis.";
RL Plant Cell 16:2089-2103(2004).
RN [5]
RP FUNCTION, AND COFACTOR.
RX PubMed=24194514; DOI=10.1074/jbc.m113.485755;
RA Hayes M.L., Giang K., Berhane B., Mulligan R.M.;
RT "Identification of two pentatricopeptide repeat genes required for RNA
RT editing and zinc binding by C-terminal cytidine deaminase-like domains.";
RL J. Biol. Chem. 288:36519-36529(2013).
CC -!- FUNCTION: Plays a major role in single RNA editing events in
CC chloroplasts. Acts as a site-recognition transacting factor involved in
CC the edition of the site 5 of ndhB1 and ndhB2 (ndhB1-5 and ndhB2-5 sites
CC corresponding to cytidine-830), which are plastid-encoded subunits of
CC the NADH-plastoquinone oxidoreductase. May provide the catalytic
CC activity for editing site conversion. {ECO:0000269|PubMed:24194514}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000269|PubMed:24194514};
CC Note=Binds 2 zinc ions per subunit. {ECO:0000269|PubMed:24194514};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the PPR family. PCMP-H subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Pentatricopeptide repeat proteins;
CC URL="https://ppr.plantenergy.uwa.edu.au";
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DR EMBL; AL035601; CAB38205.1; -; Genomic_DNA.
DR EMBL; AL161591; CAB80403.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86786.1; -; Genomic_DNA.
DR PIR; T04732; T04732.
DR RefSeq; NP_195454.1; NM_119901.2.
DR AlphaFoldDB; Q9SZT8; -.
DR SMR; Q9SZT8; -.
DR STRING; 3702.AT4G37380.1; -.
DR PaxDb; Q9SZT8; -.
DR PRIDE; Q9SZT8; -.
DR EnsemblPlants; AT4G37380.1; AT4G37380.1; AT4G37380.
DR GeneID; 829892; -.
DR Gramene; AT4G37380.1; AT4G37380.1; AT4G37380.
DR KEGG; ath:AT4G37380; -.
DR Araport; AT4G37380; -.
DR TAIR; locus:2126352; AT4G37380.
DR eggNOG; KOG4197; Eukaryota.
DR HOGENOM; CLU_002706_37_2_1; -.
DR InParanoid; Q9SZT8; -.
DR OMA; HHPILNF; -.
DR OrthoDB; 1344243at2759; -.
DR PhylomeDB; Q9SZT8; -.
DR BioCyc; ARA:AT4G37830-MON; -.
DR BioCyc; MetaCyc:AT4G37830-MON; -.
DR PRO; PR:Q9SZT8; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SZT8; baseline and differential.
DR Genevisible; Q9SZT8; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IDA:UniProtKB.
DR GO; GO:1900865; P:chloroplast RNA modification; IMP:UniProtKB.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 1.25.40.10; -; 4.
DR InterPro; IPR032867; DYW_dom.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR Pfam; PF14432; DYW_deaminase; 1.
DR Pfam; PF01535; PPR; 5.
DR Pfam; PF13041; PPR_2; 1.
DR TIGRFAMs; TIGR00756; PPR; 3.
DR PROSITE; PS51375; PPR; 10.
PE 3: Inferred from homology;
KW Chloroplast; Metal-binding; mRNA processing; Plastid; Reference proteome;
KW Repeat; RNA editing; RNA-binding; Transit peptide; Zinc.
FT TRANSIT 1..19
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 20..632
FT /note="Pentatricopeptide repeat-containing protein ELI1,
FT chloroplastic"
FT /id="PRO_0000363471"
FT REPEAT 94..128
FT /note="PPR 1"
FT REPEAT 129..159
FT /note="PPR 2"
FT REPEAT 160..194
FT /note="PPR 3"
FT REPEAT 196..221
FT /note="PPR 4"
FT REPEAT 222..256
FT /note="PPR 5"
FT REPEAT 258..292
FT /note="PPR 6"
FT REPEAT 293..323
FT /note="PPR 7"
FT REPEAT 324..354
FT /note="PPR 8"
FT REPEAT 360..395
FT /note="PPR 9"
FT REPEAT 396..426
FT /note="PPR 10"
FT REGION 431..506
FT /note="Type E motif"
FT REGION 497..512
FT /note="Required for function in RNA editing"
FT /evidence="ECO:0000269|PubMed:24194514"
FT REGION 507..537
FT /note="Type E(+) motif"
FT REGION 538..632
FT /note="Type DYW motif"
SQ SEQUENCE 632 AA; 70096 MW; 5EF31B3BD1633AC1 CRC64;
MASSPLLATS LPQNQLSTTA TARFRLPPPE KLAVLIDKSQ SVDEVLQIHA AILRHNLLLH
PRYPVLNLKL HRAYASHGKI RHSLALFHQT IDPDLFLFTA AINTASINGL KDQAFLLYVQ
LLSSEINPNE FTFSSLLKSC STKSGKLIHT HVLKFGLGID PYVATGLVDV YAKGGDVVSA
QKVFDRMPER SLVSSTAMIT CYAKQGNVEA ARALFDSMCE RDIVSWNVMI DGYAQHGFPN
DALMLFQKLL AEGKPKPDEI TVVAALSACS QIGALETGRW IHVFVKSSRI RLNVKVCTGL
IDMYSKCGSL EEAVLVFNDT PRKDIVAWNA MIAGYAMHGY SQDALRLFNE MQGITGLQPT
DITFIGTLQA CAHAGLVNEG IRIFESMGQE YGIKPKIEHY GCLVSLLGRA GQLKRAYETI
KNMNMDADSV LWSSVLGSCK LHGDFVLGKE IAEYLIGLNI KNSGIYVLLS NIYASVGDYE
GVAKVRNLMK EKGIVKEPGI STIEIENKVH EFRAGDREHS KSKEIYTMLR KISERIKSHG
YVPNTNTVLQ DLEETEKEQS LQVHSERLAI AYGLISTKPG SPLKIFKNLR VCSDCHTVTK
LISKITGRKI VMRDRNRFHH FTDGSCSCGD FW
