PP358_ARATH
ID PP358_ARATH Reviewed; 563 AA.
AC Q9SV96;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 121.
DE RecName: Full=Pentatricopeptide repeat-containing protein At4g39620, chloroplastic {ECO:0000305};
DE AltName: Full=Protein EMBRYO DEFECTIVE 2453 {ECO:0000303|PubMed:15647901};
DE Flags: Precursor;
GN Name=EMB2453 {ECO:0000303|PubMed:15647901};
GN OrderedLocusNames=At4g39620 {ECO:0000312|Araport:AT4G39620};
GN ORFNames=F23K16.250;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=15647901; DOI=10.1007/s00425-004-1452-x;
RA Cushing D.A., Forsthoefel N.R., Gestaut D.R., Vernon D.M.;
RT "Arabidopsis emb175 and other ppr knockout mutants reveal essential roles
RT for pentatricopeptide repeat (PPR) proteins in plant embryogenesis.";
RL Planta 221:424-436(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 504-563.
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [5]
RP GENE FAMILY.
RX PubMed=15269332; DOI=10.1105/tpc.104.022236;
RA Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C.,
RA Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M.,
RA Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B.,
RA Taconnat L., Small I.;
RT "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins
RT reveals their essential role in organelle biogenesis.";
RL Plant Cell 16:2089-2103(2004).
CC -!- FUNCTION: Essential for embryo development.
CC {ECO:0000269|PubMed:15647901}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9SV96-1; Sequence=Displayed;
CC -!- DISRUPTION PHENOTYPE: Embryonic lethality due to embryo development
CC arrest at early globular stage. {ECO:0000269|PubMed:15647901}.
CC -!- SIMILARITY: Belongs to the PPR family. P subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Pentatricopeptide repeat proteins;
CC URL="https://ppr.plantenergy.uwa.edu.au";
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DR EMBL; AY864349; AAW62964.1; -; Genomic_DNA.
DR EMBL; AL078620; CAB44697.1; -; Genomic_DNA.
DR EMBL; AL161595; CAB80625.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE87094.1; -; Genomic_DNA.
DR EMBL; BX826642; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; T09378; T09378.
DR RefSeq; NP_195672.1; NM_120122.2. [Q9SV96-1]
DR AlphaFoldDB; Q9SV96; -.
DR SMR; Q9SV96; -.
DR STRING; 3702.AT4G39620.1; -.
DR PaxDb; Q9SV96; -.
DR PRIDE; Q9SV96; -.
DR ProteomicsDB; 249249; -. [Q9SV96-1]
DR EnsemblPlants; AT4G39620.1; AT4G39620.1; AT4G39620. [Q9SV96-1]
DR GeneID; 830116; -.
DR Gramene; AT4G39620.1; AT4G39620.1; AT4G39620. [Q9SV96-1]
DR KEGG; ath:AT4G39620; -.
DR Araport; AT4G39620; -.
DR TAIR; locus:2122561; AT4G39620.
DR eggNOG; KOG4197; Eukaryota.
DR InParanoid; Q9SV96; -.
DR OrthoDB; 1344243at2759; -.
DR PhylomeDB; Q9SV96; -.
DR PRO; PR:Q9SV96; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SV96; baseline and differential.
DR Genevisible; Q9SV96; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0003729; F:mRNA binding; IEA:InterPro.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR Gene3D; 1.25.40.10; -; 3.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR044179; PPR5-like.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR47874; PTHR47874; 1.
DR Pfam; PF01535; PPR; 4.
DR Pfam; PF13041; PPR_2; 2.
DR TIGRFAMs; TIGR00756; PPR; 6.
DR PROSITE; PS51375; PPR; 9.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chloroplast; Plastid; Reference proteome; Repeat;
KW Transit peptide.
FT TRANSIT 1..47
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 48..563
FT /note="Pentatricopeptide repeat-containing protein
FT At4g39620, chloroplastic"
FT /id="PRO_0000363475"
FT REPEAT 132..166
FT /note="PPR 1"
FT REPEAT 167..197
FT /note="PPR 2"
FT REPEAT 207..241
FT /note="PPR 3"
FT REPEAT 242..276
FT /note="PPR 4"
FT REPEAT 277..311
FT /note="PPR 5"
FT REPEAT 312..346
FT /note="PPR 6"
FT REPEAT 347..381
FT /note="PPR 7"
FT REPEAT 382..416
FT /note="PPR 8"
FT REPEAT 417..451
FT /note="PPR 9"
FT REGION 468..501
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 520..551
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 520..537
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 563 AA; 65302 MW; 71F3A81F8B570057 CRC64;
MDYLLTSPSS LRFSDFISSI PKETDHKWLR FSVNLGDARR STRTRITCGA ISSRRKLAER
ESAERENRVL VRSLMSRISD REPLVKTLDK YVKVVRCDHC FLLFEELGKS DKWLQCLEVF
RWMQKQRWYI PDNGVYSKLI SVMGKKGQTR MAMWLFSEMK NSGCRPDASV YNALITAHLH
TRDKAKALEK VRGYLDKMKG IERCQPNVVT YNILLRAFAQ SGKVDQVNAL FKDLDMSPVS
PDVYTFNGVM DAYGKNGMIK EMEAVLTRMR SNECKPDIIT FNVLIDSYGK KQEFEKMEQT
FKSLMRSKEK PTLPTFNSMI INYGKARMID KAEWVFKKMN DMNYIPSFIT YECMIMMYGY
CGSVSRAREI FEEVGESDRV LKASTLNAML EVYCRNGLYI EADKLFHNAS AFRVHPDAST
YKFLYKAYTK ADMKEQVQIL MKKMEKDGIV PNKRFFLEAL EVFGSRLPGS GSENRKSTRS
SRSRDSPKGR GGNQLTEFQD KDNKHVFRAV QDCRKNYLEN LSGHDKGSRD ESRKPSQEKQ
PLFASDQNNM MIKEKKELFT RVL
