ID   PP4R2_RABIT             Reviewed;          78 AA.
AC   P0C5B7;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   25-MAY-2022, entry version 29.
DE   RecName: Full=Serine/threonine-protein phosphatase 4 regulatory subunit 2;
DE   Flags: Fragments;
GN   Name=PPP4R2;
OS   Oryctolagus cuniculus (Rabbit).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Lagomorpha; Leporidae; Oryctolagus.
OX   NCBI_TaxID=9986;
RN   [1]
RP   PROTEIN SEQUENCE, AND INTERACTION WITH PPP4C.
RX   PubMed=10769191; DOI=10.1042/bj3470845;
RA   Hastie C.J., Carnegie G.K., Morrice N., Cohen P.T.W.;
RT   "A novel 50 kDa protein forms complexes with protein phosphatase 4 and is
RT   located at centrosomal microtubule organizing centres.";
RL   Biochem. J. 347:845-855(2000).
CC   -!- FUNCTION: Regulatory subunit of serine/threonine-protein phosphatase 4
CC       (PP4). May regulate the activity of PPP4C at centrosomal microtubule
CC       organizing centers. Its interaction with the SMN complex leads to
CC       enhance the temporal localization of snRNPs, suggesting a role of PPP4C
CC       in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4
CC       complex specifically dephosphorylates H2AX phosphorylated on 'Ser-140'
CC       (gamma-H2AX) generated during DNA replication and required for DNA
CC       double strand break repair (By similarity). Mediates RPA2
CC       dephosphorylation by recruiting PPP4C to RPA2 in a DNA damage-dependent
CC       manner. RPA2 dephosphorylation is required for the efficient RPA2-
CC       mediated recruitment of RAD51 to chromatin following double strand
CC       breaks, an essential step for DNA repair (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Serine/threonine-protein phosphatase 4 (PP4) occurs in
CC       different assemblies of the catalytic and one or more regulatory
CC       subunits. Component of the PP4 complexes PPP4C-PPP4R2, PPP4C-PPP4R2-
CC       PPP4R3A and PPP4C-PPP4R2-PPP4R3B. The PPP4C-PPP4R2 complex appears to
CC       be a tetramer composed of 2 molecules of PPP4C and 2 molecules of
CC       PPP4R2. Interacts with DDX20/GEMIN3 and GEMIN4 (By similarity).
CC       Interacts with RPA2; this DNA damage-dependent interaction recruits
CC       PPP4C leading to RPA2 dephosphorylation (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC       center, centrosome {ECO:0000250}. Nucleus {ECO:0000250}. Note=Ionizing
CC       radiation induces relocalization to nuclear foci and colocalization
CC       with RPA2. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PPP4R2 family. {ECO:0000305}.
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DR   AlphaFoldDB; P0C5B7; -.
DR   InParanoid; P0C5B7; -.
DR   Proteomes; UP000001811; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0030289; C:protein phosphatase 4 complex; ISS:UniProtKB.
DR   GO; GO:0019888; F:protein phosphatase regulator activity; ISS:UniProtKB.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0010569; P:regulation of double-strand break repair via homologous recombination; ISS:UniProtKB.
DR   GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cytoplasm; Cytoskeleton; Direct protein sequencing; mRNA processing;
KW   mRNA splicing; Nucleus; Reference proteome.
FT   CHAIN           <1..>78
FT                   /note="Serine/threonine-protein phosphatase 4 regulatory
FT                   subunit 2"
FT                   /id="PRO_0000299368"
FT   REGION          53..78
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   NON_CONS        11..12
FT                   /evidence="ECO:0000305"
FT   NON_CONS        23..24
FT                   /evidence="ECO:0000305"
FT   NON_CONS        39..40
FT                   /evidence="ECO:0000305"
FT   NON_CONS        44..45
FT                   /evidence="ECO:0000305"
FT   NON_CONS        51..52
FT                   /evidence="ECO:0000305"
FT   NON_CONS        64..65
FT                   /evidence="ECO:0000305"
FT   NON_TER         1
FT   NON_TER         78
SQ   SEQUENCE   78 AA;  9047 MW;  59F30142E559A070 CRC64;
     LQEALKDFEK RTGETMIQWS QFKGPPNPNV EYIPFDEMKN VMVVMNGVMF PINGPGTPRP
     LNRPNLQQNE EKNHHPDE