ID   PP4R3_DANRE             Reviewed;         818 AA.
AC   Q5SP90;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Serine/threonine-protein phosphatase 4 regulatory subunit 3;
DE   AltName: Full=SMEK homolog 1;
GN   Name=smek1; ORFNames=si:dkey-65b13.2;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [2]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-769 AND SER-770, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Embryo;
RX   PubMed=18307296; DOI=10.1021/pr700667w;
RA   Lemeer S., Pinkse M.W.H., Mohammed S., van Breukelen B., den Hertog J.,
RA   Slijper M., Heck A.J.R.;
RT   "Online automated in vivo zebrafish phosphoproteomics: from large-scale
RT   analysis down to a single embryo.";
RL   J. Proteome Res. 7:1555-1564(2008).
CC   -!- FUNCTION: Regulatory subunit of serine/threonine-protein phosphatase 4.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Serine/threonine-protein phosphatase 4 (PP4) occurs in
CC       different assemblies of the catalytic and one or more regulatory
CC       subunits. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the SMEK family. {ECO:0000305}.
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DR   EMBL; AL929220; CAI21197.1; -; Genomic_DNA.
DR   RefSeq; NP_001038274.1; NM_001044809.1.
DR   AlphaFoldDB; Q5SP90; -.
DR   STRING; 7955.ENSDARP00000061842; -.
DR   iPTMnet; Q5SP90; -.
DR   PaxDb; Q5SP90; -.
DR   PRIDE; Q5SP90; -.
DR   Ensembl; ENSDART00000061843; ENSDARP00000061842; ENSDARG00000042187.
DR   GeneID; 558578; -.
DR   KEGG; dre:558578; -.
DR   CTD; 558578; -.
DR   ZFIN; ZDB-GENE-030131-3958; smek1.
DR   eggNOG; KOG2175; Eukaryota.
DR   GeneTree; ENSGT00390000018199; -.
DR   HOGENOM; CLU_004909_3_0_1; -.
DR   InParanoid; Q5SP90; -.
DR   OMA; MMRGYML; -.
DR   OrthoDB; 388216at2759; -.
DR   PhylomeDB; Q5SP90; -.
DR   TreeFam; TF315190; -.
DR   PRO; PR:Q5SP90; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 20.
DR   Bgee; ENSDARG00000042187; Expressed in early embryo and 29 other tissues.
DR   GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR   GO; GO:0030289; C:protein phosphatase 4 complex; IBA:GO_Central.
DR   GO; GO:0072542; F:protein phosphatase activator activity; IBA:GO_Central.
DR   GO; GO:0006974; P:cellular response to DNA damage stimulus; IBA:GO_Central.
DR   GO; GO:0006470; P:protein dephosphorylation; IBA:GO_Central.
DR   Gene3D; 2.30.29.30; -; 1.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR006887; DUF625.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   Pfam; PF04802; SMK-1; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
PE   1: Evidence at protein level;
KW   Phosphoprotein; Reference proteome.
FT   CHAIN           1..818
FT                   /note="Serine/threonine-protein phosphatase 4 regulatory
FT                   subunit 3"
FT                   /id="PRO_0000254600"
FT   DOMAIN          1..100
FT                   /note="WH1"
FT   REGION          670..818
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        682..720
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        722..776
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        787..804
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         769
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18307296"
FT   MOD_RES         770
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18307296"
SQ   SEQUENCE   818 AA;  93789 MW;  3F6000A42BD25866 CRC64;
     MTDTRRRVKV YTLNEDRQWD DRGTGHVSSA YVERLKGMSL LVRAESDGSL LLESKINPNT
     AYQKQQDTLI VWSEAENYDL ALSFQEKAGC DEIWEKICQV QGKDPSVDIT QELIDESEEE
     RFDDMSSPGL ELPPCELSRL EEVAELVASS LPSPLRREKL ALALENEGYI RKLLELFRVC
     EDLENREGLH HLYDIIKGIF LLNRTALFEV MFSEECIMDV IGCLEFDPSL PQPRRHREFL
     TTTARFKEVI PISDPELRQK IHQTYRVQYI QDMVLPTPSV FEENMLSTLH SFIFFNKVEI
     VGMLQDDEKF LTELFAQLTD EATDDDKRHE LVNFLKEFCA FSQTLQPQNR DAFFKTLSNM
     GILQALEVIL GMDDVQVRGA ATDIFSYLVE YNPSMVREFV MQESQQNDDD ILLINLIIEH
     MICDTDPELG GAVQLMGLLR TLVDPENMLA TANKTEKTEF LSFFYKHCMH VLSAPLLANT
     TEEKPSKDDF QTCQLLALIV ELLTFCVEHH TYHIKNYIIN KDILRRVLVL TASQHAFLAL
     CALRFMRKII GLKDEFYNRY IMRNFLFEPV VKAFLNNGSR YNLINSAIIE MFEYVRVEDV
     KSLTAHIIEN YWKGLEDVDY VQTFKGLKLR YEQQRERQDN PKLDSMRSIL RNHRFRRDAR
     TLEDEEEMWF NTDEEDLEDG EAVVPPSDKM KNDEDLMDPI SKFMERKKLK DSEEKEVLTG
     KASLSGRQSP SFKLSFSSSP KASLSSPPTA SLHPGSPGSP SSPGTGARSS PPSAAVTTKG
     GLVGLVDYPD DDEEDEDEED ADSKEESPPL SKKSKLSS