PP4R4_HUMAN
ID PP4R4_HUMAN Reviewed; 873 AA.
AC Q6NUP7; Q9BUF8; Q9HCF0;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Serine/threonine-protein phosphatase 4 regulatory subunit 4;
GN Name=PPP4R4; Synonyms=KIAA1622, PP4R4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=10997877; DOI=10.1093/dnares/7.4.271;
RA Nagase T., Kikuno R., Nakayama M., Hirosawa M., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XVIII. The
RT complete sequences of 100 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 7:273-281(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP INTERACTION WITH PPP4C, IDENTIFICATION IN THE PPP4C-PPP4R4 COMPLEX,
RP SUBCELLULAR LOCATION, AND MUTAGENESIS OF ARG-501 AND VAL-618.
RX PubMed=18715871; DOI=10.1074/jbc.m803443200;
RA Chen G.I., Tisayakorn S., Jorgensen C., D'Ambrosio L.M., Goudreault M.,
RA Gingras A.-C.;
RT "PP4R4/KIAA1622 forms a novel stable cytosolic complex with phosphoprotein
RT phosphatase 4.";
RL J. Biol. Chem. 283:29273-29284(2008).
CC -!- FUNCTION: Putative regulatory subunit of serine/threonine-protein
CC phosphatase 4.
CC -!- SUBUNIT: Serine/threonine-protein phosphatase 4 (PP4) occurs in
CC different assemblies of the catalytic and one or more regulatory
CC subunits. Component of the PP4 complex PPP4C-PPP4R4.
CC {ECO:0000269|PubMed:18715871}.
CC -!- INTERACTION:
CC Q6NUP7; P60510: PPP4C; NbExp=8; IntAct=EBI-1774189, EBI-1046072;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18715871}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6NUP7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6NUP7-2; Sequence=VSP_029616, VSP_029617;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB13448.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB046842; BAB13448.1; ALT_INIT; mRNA.
DR EMBL; CH471061; EAW81561.1; -; Genomic_DNA.
DR EMBL; CH471061; EAW81562.1; -; Genomic_DNA.
DR EMBL; BC002650; AAH02650.2; -; mRNA.
DR EMBL; BC068491; AAH68491.1; -; mRNA.
DR CCDS; CCDS9921.1; -. [Q6NUP7-1]
DR CCDS; CCDS9922.1; -. [Q6NUP7-2]
DR RefSeq; NP_066009.2; NM_020958.2. [Q6NUP7-2]
DR RefSeq; NP_478144.1; NM_058237.1. [Q6NUP7-1]
DR AlphaFoldDB; Q6NUP7; -.
DR BioGRID; 121741; 10.
DR ComplexPortal; CPX-1845; PPP4C-PPP4R4 protein phosphatase 4 complex.
DR IntAct; Q6NUP7; 3.
DR MINT; Q6NUP7; -.
DR STRING; 9606.ENSP00000305924; -.
DR GlyGen; Q6NUP7; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q6NUP7; -.
DR PhosphoSitePlus; Q6NUP7; -.
DR BioMuta; PPP4R4; -.
DR DMDM; 74736832; -.
DR EPD; Q6NUP7; -.
DR jPOST; Q6NUP7; -.
DR MassIVE; Q6NUP7; -.
DR MaxQB; Q6NUP7; -.
DR PaxDb; Q6NUP7; -.
DR PeptideAtlas; Q6NUP7; -.
DR PRIDE; Q6NUP7; -.
DR ProteomicsDB; 66699; -. [Q6NUP7-1]
DR ProteomicsDB; 66700; -. [Q6NUP7-2]
DR Antibodypedia; 49053; 48 antibodies from 18 providers.
DR DNASU; 57718; -.
DR Ensembl; ENST00000304338.8; ENSP00000305924.3; ENSG00000119698.12. [Q6NUP7-1]
DR Ensembl; ENST00000328839.3; ENSP00000330831.3; ENSG00000119698.12. [Q6NUP7-2]
DR Ensembl; ENST00000614957.2; ENSP00000482849.1; ENSG00000278326.4. [Q6NUP7-2]
DR Ensembl; ENST00000619905.4; ENSP00000480902.1; ENSG00000278326.4. [Q6NUP7-1]
DR GeneID; 57718; -.
DR KEGG; hsa:57718; -.
DR MANE-Select; ENST00000304338.8; ENSP00000305924.3; NM_058237.2; NP_478144.1.
DR UCSC; uc001ycr.4; human. [Q6NUP7-1]
DR CTD; 57718; -.
DR DisGeNET; 57718; -.
DR GeneCards; PPP4R4; -.
DR HGNC; HGNC:23788; PPP4R4.
DR HPA; ENSG00000119698; Tissue enhanced (retina, testis).
DR neXtProt; NX_Q6NUP7; -.
DR OpenTargets; ENSG00000119698; -.
DR PharmGKB; PA164724938; -.
DR VEuPathDB; HostDB:ENSG00000119698; -.
DR eggNOG; KOG0211; Eukaryota.
DR GeneTree; ENSGT00510000047895; -.
DR HOGENOM; CLU_010601_0_0_1; -.
DR InParanoid; Q6NUP7; -.
DR OMA; NEREAEC; -.
DR OrthoDB; 447572at2759; -.
DR PhylomeDB; Q6NUP7; -.
DR TreeFam; TF313717; -.
DR PathwayCommons; Q6NUP7; -.
DR SignaLink; Q6NUP7; -.
DR BioGRID-ORCS; 57718; 8 hits in 1065 CRISPR screens.
DR ChiTaRS; PPP4R4; human.
DR GenomeRNAi; 57718; -.
DR Pharos; Q6NUP7; Tdark.
DR PRO; PR:Q6NUP7; -.
DR Proteomes; UP000005640; Chromosome 14.
DR RNAct; Q6NUP7; protein.
DR Bgee; ENSG00000119698; Expressed in superior frontal gyrus and 94 other tissues.
DR ExpressionAtlas; Q6NUP7; baseline and differential.
DR Genevisible; Q6NUP7; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0008287; C:protein serine/threonine phosphatase complex; IDA:UniProtKB.
DR GO; GO:0019888; F:protein phosphatase regulator activity; IDA:MGI.
DR GO; GO:0001835; P:blastocyst hatching; IEA:Ensembl.
DR GO; GO:0032515; P:negative regulation of phosphoprotein phosphatase activity; IDA:MGI.
DR GO; GO:0080163; P:regulation of protein serine/threonine phosphatase activity; IDA:MGI.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR021133; HEAT_type_2.
DR InterPro; IPR039918; PPP4R4.
DR PANTHER; PTHR21467; PTHR21467; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50077; HEAT_REPEAT; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm; Phosphoprotein;
KW Reference proteome; Repeat.
FT CHAIN 1..873
FT /note="Serine/threonine-protein phosphatase 4 regulatory
FT subunit 4"
FT /id="PRO_0000311850"
FT REPEAT 213..251
FT /note="HEAT 1"
FT REPEAT 252..290
FT /note="HEAT 2"
FT REPEAT 392..427
FT /note="HEAT 3"
FT REGION 713..766
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 822..873
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 686..720
FT /evidence="ECO:0000255"
FT COMPBIAS 713..739
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 740..764
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 822..861
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 775
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8C0Y0"
FT MOD_RES 797
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q8C0Y0"
FT VAR_SEQ 99..120
FT /note="EALHVAGVEMQLTAAMSFLTIL -> VHEDAHLFIQRVWISHMFVQRV (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_029616"
FT VAR_SEQ 121..873
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_029617"
FT MUTAGEN 501
FT /note="R->W: Abolishes interaction with PPP4C."
FT /evidence="ECO:0000269|PubMed:18715871"
FT MUTAGEN 618
FT /note="V->A: Diminishes interaction with PPP4C."
FT /evidence="ECO:0000269|PubMed:18715871"
FT MUTAGEN 618
FT /note="V->D: Abolishes interaction with PPP4C."
FT /evidence="ECO:0000269|PubMed:18715871"
SQ SEQUENCE 873 AA; 99452 MW; 9C1A9CD4584447C1 CRC64;
MHPPPPAAAM DFSQNSLFGY MEDLQELTII ERPVRRSLKT PEEIERLTVD EDLSDIERAV
YLLSAGQDVQ GTSVIANLPF LMRQNPTETL RRVLPKVREA LHVAGVEMQL TAAMSFLTIL
QDESVSIHAY THSFLQVILL HLEHRDTGVS NAWLETLLSV IEVLPKETLR HEILNPLVSK
AQLSQTVQSR LVSCKILGKL TNKFDAHTIK REILPLVKSL CQDVEYEVRS CMCRQLENIA
QGIGTELTKS VVLPELIELS RDEGSSVRLA AFETLVNLLD IFDTDDRSQT ILPLVKSFCE
KSFKADESIL ISLSFHLGKL CHGLYGIFTP DQHLRFLEFY KKLCTLGLQQ ENGHNENQIP
PQILEQEKKY ISVRKNCAYN FPAMIVFVDP KNFHMELYST FFCLCHDPEV PVRYTIAICF
YEVSKLLNSG VYLIHKELIT LLQDESLEVL DALIDHLPEI LELMSTGGES SVQENKLSSL
PDLIPALTAA EQRAAASLKW RTHEKLLQKY ACLPHVISSD QIYYRFLQRM FTIMMTNNVL
PVQKAASRTL CIFLRYNRKQ EQRHEVIQKL IEQLGQGKSY WNRLRFLDTC EFIIEIFSKS
FFCKYFFLPA IELTHDPVAN VRMKLCYLLP KVKSTLKIPA DKHLLQQLEM CVRKLLCQEK
DKDVLAIVKR TVLELDRMEM SMDAFQKKFY EKDLLDQEKE REELLLLEME QLEKEKQQND
GRPMSDKMFE KKRRDTKTPT QSLPKNIPIS VPGPSSVTPS TSKEIKKSKL IRSQSFNNQA
FHAKYGNLEK CASKSSTTGY TTSVSGLGKT SVLSLADDSF RTRNASSVPS SFSPNTPLPS
TSRGTGNSVD PKSSGSKDTQ PRKATLKSRK SNP
