ID   PP4RS_CAEEL             Reviewed;         327 AA.
AC   Q9N4E9;
DT   25-APR-2018, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 133.
DE   RecName: Full=Serine/threonine-protein phosphatase 4 regulatory subunit ppfr-4 {ECO:0000305};
GN   Name=ppfr-4 {ECO:0000303|PubMed:19087961, ECO:0000312|WormBase:Y71H2B.3};
GN   ORFNames=Y71H2B.3 {ECO:0000312|WormBase:Y71H2B.3};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN   [1] {ECO:0000312|Proteomes:UP000001940}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2] {ECO:0000305}
RP   FUNCTION, SUBUNIT, AND DISRUPTION PHENOTYPE.
RX   PubMed=19087961; DOI=10.1534/genetics.108.096016;
RA   Han X., Gomes J.E., Birmingham C.L., Pintard L., Sugimoto A., Mains P.E.;
RT   "The role of protein phosphatase 4 in regulating microtubule severing in
RT   the Caenorhabditis elegans embryo.";
RL   Genetics 181:933-943(2009).
CC   -!- FUNCTION: Probable regulatory subunit of serine/threonine-protein
CC       phosphatase PP4 which may play a role in meiosis and embryonic mitosis.
CC       Probably in association with catalytic subunit pph-4.1, regulates
CC       microtubule severing during oocyte meiosis II by dephosphorylating and
CC       likely activating mei-1, a component of the katanin microtubule
CC       severing complex. {ECO:0000269|PubMed:19087961}.
CC   -!- SUBUNIT: Serine/threonine-protein phosphatase 4 (PP4) occurs in
CC       different assemblies of the catalytic and one or more regulatory
CC       subunits (By similarity). The catalytic subunit is likely to be pph-4.1
CC       (PubMed:19087961). {ECO:0000250|UniProtKB:Q8TF05,
CC       ECO:0000305|PubMed:19087961}.
CC   -!- INTERACTION:
CC       Q9N4E9; Q9XW79: pph-4.1; NbExp=3; IntAct=EBI-331766, EBI-331742;
CC   -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown causes 94 percent
CC       embryonic lethality when injected compared to 20 percent when fed.
CC       Partially rescues embryonic lethality in gain of function mei-1 (ct46)
CC       mutant background. {ECO:0000269|PubMed:19087961}.
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DR   EMBL; BX284603; CCD72402.1; -; Genomic_DNA.
DR   RefSeq; NP_497591.1; NM_065190.4.
DR   AlphaFoldDB; Q9N4E9; -.
DR   SMR; Q9N4E9; -.
DR   DIP; DIP-27072N; -.
DR   IntAct; Q9N4E9; 5.
DR   STRING; 6239.Y71H2B.3; -.
DR   EPD; Q9N4E9; -.
DR   PaxDb; Q9N4E9; -.
DR   PeptideAtlas; Q9N4E9; -.
DR   EnsemblMetazoa; Y71H2B.3.1; Y71H2B.3.1; WBGene00022193.
DR   GeneID; 190617; -.
DR   UCSC; Y71H2B.3; c. elegans.
DR   CTD; 190617; -.
DR   WormBase; Y71H2B.3; CE24630; WBGene00022193; ppfr-4.
DR   eggNOG; KOG2830; Eukaryota.
DR   GeneTree; ENSGT00390000002414; -.
DR   HOGENOM; CLU_041824_1_0_1; -.
DR   InParanoid; Q9N4E9; -.
DR   OMA; RAWDEFT; -.
DR   OrthoDB; 1348942at2759; -.
DR   PhylomeDB; Q9N4E9; -.
DR   SignaLink; Q9N4E9; -.
DR   PRO; PR:Q9N4E9; -.
DR   Proteomes; UP000001940; Chromosome III.
DR   Bgee; WBGene00022193; Expressed in adult organism and 3 other tissues.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0051721; F:protein phosphatase 2A binding; IBA:GO_Central.
DR   GO; GO:0019888; F:protein phosphatase regulator activity; ISS:WormBase.
DR   GO; GO:0009792; P:embryo development ending in birth or egg hatching; IGI:UniProtKB.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:1903361; P:protein localization to basolateral plasma membrane; IMP:WormBase.
DR   GO; GO:0035303; P:regulation of dephosphorylation; IBA:GO_Central.
DR   GO; GO:0009966; P:regulation of signal transduction; IEA:InterPro.
DR   Gene3D; 1.25.40.540; -; 1.
DR   InterPro; IPR038511; TAP42/TAP46-like_sf.
DR   InterPro; IPR007304; TAP46-like.
DR   PANTHER; PTHR10933; PTHR10933; 1.
DR   Pfam; PF04177; TAP42; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Meiosis; Reference proteome.
FT   CHAIN           1..327
FT                   /note="Serine/threonine-protein phosphatase 4 regulatory
FT                   subunit ppfr-4"
FT                   /id="PRO_0000443999"
FT   REGION          271..327
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          141..185
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        274..288
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        300..327
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   327 AA;  37947 MW;  7E5003DB5EE0FFCD CRC64;
     MSELSDEEIS LQALYDPSKK VIGDIEDGIF STPELQPRIK TGIDNLQLVT KLVNQMRLFS
     SNEQIEDVPT NSLPYLLVPC FLGILHQNLM TEPGLKLDEL RKSKIYMRNF LDRLRDLCLI
     TTRLPWEDED TEEQNLKEKP KLAVEEIRRL KLERHKKKQE LKMAELRIQK QLEAVSIDEQ
     NLRELYITQL LFWSERCYEE LQAIDDELPL LKMMAERASH PHRHPAPPPA TKTVPTLKPF
     IITRDAQQKQ VFGLGYPGIP AMSVDEWYHQ KFGHNPQNAP QSSAPAGAEA QESEEEVDDD
     EARAKAMRWD EYKDDHRRGW GNMHNKG