PP6R1_MOUSE
ID PP6R1_MOUSE Reviewed; 856 AA.
AC Q7TSI3; Q80TJ4;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Serine/threonine-protein phosphatase 6 regulatory subunit 1;
DE AltName: Full=SAPS domain family member 1;
GN Name=Ppp6r1; Synonyms=Kiaa1115, Pp6r1, Saps1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=12693553; DOI=10.1093/dnares/10.1.35;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S.,
RA Nakajima D., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: II.
RT The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:35-48(2003).
RN [2]
RP SEQUENCE REVISION.
RA Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H.;
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=16769727; DOI=10.1074/jbc.m601772200;
RA Stefansson B., Brautigan D.L.;
RT "Protein phosphatase 6 subunit with conserved Sit4-associated protein
RT domain targets IkappaBepsilon.";
RL J. Biol. Chem. 281:22624-22634(2006).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-529; SER-530 AND SER-531, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232; SER-529; SER-530;
RP SER-531; SER-633; SER-636 AND SER-826, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Regulatory subunit of protein phosphatase 6 (PP6). May
CC function as a scaffolding PP6 subunit. Involved in the PP6-mediated
CC dephosphorylation of NFKBIE opposing its degradation in response to
CC TNF-alpha (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Protein phosphatase 6 (PP6) holoenzyme is proposed to be a
CC heterotrimeric complex formed of the catalytic subunit, a SAPS domain-
CC containing subunit (PP6R) and an ankyrin repeat-domain containing
CC regulatory subunit (ARS). Interacts with PPP6C and NFKBIE. Interacts
CC with ANKRD28, ANKRD44 and ANKRD52 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Ubiquitous with highest expression in lung, spleen
CC and bladder. {ECO:0000269|PubMed:16769727}.
CC -!- SIMILARITY: Belongs to the SAPS family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC65732.3; Type=Miscellaneous discrepancy; Note=Derived from pre-RNA.; Evidence={ECO:0000305};
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DR EMBL; AK122450; BAC65732.3; ALT_SEQ; Transcribed_RNA.
DR EMBL; BC053076; AAH53076.1; -; mRNA.
DR CCDS; CCDS39737.1; -.
DR RefSeq; NP_766482.2; NM_172894.2.
DR RefSeq; XP_011248858.1; XM_011250556.2.
DR AlphaFoldDB; Q7TSI3; -.
DR SMR; Q7TSI3; -.
DR BioGRID; 232560; 26.
DR IntAct; Q7TSI3; 27.
DR STRING; 10090.ENSMUSP00000066736; -.
DR iPTMnet; Q7TSI3; -.
DR PhosphoSitePlus; Q7TSI3; -.
DR EPD; Q7TSI3; -.
DR jPOST; Q7TSI3; -.
DR MaxQB; Q7TSI3; -.
DR PaxDb; Q7TSI3; -.
DR PRIDE; Q7TSI3; -.
DR ProteomicsDB; 289798; -.
DR Antibodypedia; 33046; 67 antibodies from 20 providers.
DR Ensembl; ENSMUST00000064099; ENSMUSP00000066736; ENSMUSG00000052296.
DR GeneID; 243819; -.
DR KEGG; mmu:243819; -.
DR UCSC; uc009eyb.1; mouse.
DR CTD; 22870; -.
DR MGI; MGI:2442163; Ppp6r1.
DR VEuPathDB; HostDB:ENSMUSG00000052296; -.
DR eggNOG; KOG2073; Eukaryota.
DR GeneTree; ENSGT00390000009899; -.
DR HOGENOM; CLU_012598_0_0_1; -.
DR InParanoid; Q7TSI3; -.
DR OMA; NAEQGPN; -.
DR OrthoDB; 329232at2759; -.
DR PhylomeDB; Q7TSI3; -.
DR TreeFam; TF313227; -.
DR Reactome; R-MMU-204005; COPII-mediated vesicle transport.
DR BioGRID-ORCS; 243819; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Ppp6r1; mouse.
DR PRO; PR:Q7TSI3; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q7TSI3; protein.
DR Bgee; ENSMUSG00000052296; Expressed in dorsal pancreas and 188 other tissues.
DR ExpressionAtlas; Q7TSI3; baseline and differential.
DR Genevisible; Q7TSI3; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0019903; F:protein phosphatase binding; ISO:MGI.
DR GO; GO:0019888; F:protein phosphatase regulator activity; IBA:GO_Central.
DR GO; GO:0031267; F:small GTPase binding; ISO:MGI.
DR GO; GO:0043666; P:regulation of phosphoprotein phosphatase activity; ISO:MGI.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR007587; SAPS.
DR PANTHER; PTHR12634; PTHR12634; 1.
DR Pfam; PF04499; SAPS; 2.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..856
FT /note="Serine/threonine-protein phosphatase 6 regulatory
FT subunit 1"
FT /id="PRO_0000046097"
FT REGION 10..403
FT /note="Interaction with PPP6C"
FT /evidence="ECO:0000250"
FT REGION 621..770
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 792..856
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 646..663
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 667..683
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 731..764
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 792..823
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 830..856
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 232
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 524
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9UPN7"
FT MOD_RES 529
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 530
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 531
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 633
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 636
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 698
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UPN7"
FT MOD_RES 739
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UPN7"
FT MOD_RES 826
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 309
FT /note="D -> N (in Ref. 1; BAC65732)"
FT /evidence="ECO:0000305"
FT CONFLICT 683..685
FT /note="AEQ -> G (in Ref. 1; BAC65732)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 856 AA; 94527 MW; CC6D541FDBBDBC1A CRC64;
MFWKFDLHTS SHLDTLLEKE DLSLPELLDE EDVLQECKVV NRKLLDFLLQ PSHLQAMVAW
VTQEPPASGE ERLRYKYPSV ACEILTSDVP QINDALGADE SLLNRLYGFL QSGDSLNPLL
ASFFSKVMGI LINRKTDQLV SFLRKKDDFV DLLLRHIGTS AIMDLLLRLL TCVERPQLRQ
DVFNWLNEEK IVQRLIEQIH PSKDDNQHSN ASQSLCDIIR LSREQMIQGQ DSPEPDQLLA
TLEKQETIEQ LLSNMFEGEQ CQSVIVSGIQ VLLTLLEPRR PRSDSVTMNN FFSSVDGQLE
LLAQGALDDA LSSMGALHAL RPRLDRFHQL LLEPPKLEPL QMTWGSLAPP LGNTRLHVVK
LLASALSANA AALTQELLVL DVPNTLLDLF FHYVFNNFLH AQVEVCVSAM LSSGPPPDSS
SETPVPNPIV KHLLQHCRLV ERILASWEEN DRVQSGGGPR KGYMGHLTRV ANAVVQNAEQ
GPNAEQLGQL LKELPEEQQQ RWEAFVSGPL AETNKKNTVD LVNTHHLHSS SDDEDDRLKE
FNFPEEAVLQ QAFMDFQMQR MTSAFIDHFG FNDEEFGEQE ESVNAPFDKT ANITFSLNAD
DENPNANLLE ICYKDRIQQF DDEEEEEEEG QGSAESDGEY GAWQGSQPVR ASQASQPPGV
RSGGSTDSEE EDEEEDEEED EGAEQAACGR TSPSSFPSPS TQPPGPSWTA TFDTVPMDAP
TGPPVSKEAD MSSIQIPSSP PAHGSPQLRS QDPTHPSAPQ EVTDSSKVAE PLAPCQALVS
VADVQATLHG MRSAPSSLDS ATRDPSTSVP DFKAHQSPQT MEGKRSPEHL GLPQSQSALE
MPNGSTPGGP ISSGSQ
