PP6R2_MOUSE
ID PP6R2_MOUSE Reviewed; 923 AA.
AC Q8R3Q2; Q9CXB7;
DT 25-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 25-MAY-2022, entry version 127.
DE RecName: Full=Serine/threonine-protein phosphatase 6 regulatory subunit 2;
DE AltName: Full=SAPS domain family member 2;
GN Name=Ppp6r2; Synonyms=Kiaa0685, Pp6r2, Saps2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-206.
RC STRAIN=C57BL/6J; TISSUE=Embryonic lung;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic brain;
RX PubMed=15345747; DOI=10.1074/mcp.m400085-mcp200;
RA Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.;
RT "Phosphoproteomic analysis of the developing mouse brain.";
RL Mol. Cell. Proteomics 3:1093-1101(2004).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=16769727; DOI=10.1074/jbc.m601772200;
RA Stefansson B., Brautigan D.L.;
RT "Protein phosphatase 6 subunit with conserved Sit4-associated protein
RT domain targets IkappaBepsilon.";
RL J. Biol. Chem. 281:22624-22634(2006).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-746, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Lung, Pancreas, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Regulatory subunit of protein phosphatase 6 (PP6). May
CC function as a scaffolding PP6 subunit. Involved in the PP6-mediated
CC dephosphorylation of NFKBIE opposing its degradation in response to
CC TNF-alpha.
CC -!- SUBUNIT: Protein phosphatase 6 (PP6) holoenzyme is proposed to be a
CC heterotrimeric complex formed by the catalytic subunit, a SAPS domain-
CC containing subunit (PP6R) and an ankyrin repeat-domain containing
CC regulatory subunit (ARS). Interacts with PPP6C and NFKBIE. Interacts
CC with ANKRD28 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Strongest expression in bladder and lower levels
CC found in heart and pancreas. Very weak expression observed in all other
CC tissues tested. {ECO:0000269|PubMed:16769727}.
CC -!- SIMILARITY: Belongs to the SAPS family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AK018403; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; BC024879; AAH24879.1; -; mRNA.
DR EMBL; AK018403; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS27743.1; -.
DR AlphaFoldDB; Q8R3Q2; -.
DR SMR; Q8R3Q2; -.
DR IntAct; Q8R3Q2; 3.
DR STRING; 10090.ENSMUSP00000086167; -.
DR iPTMnet; Q8R3Q2; -.
DR PhosphoSitePlus; Q8R3Q2; -.
DR EPD; Q8R3Q2; -.
DR jPOST; Q8R3Q2; -.
DR MaxQB; Q8R3Q2; -.
DR PaxDb; Q8R3Q2; -.
DR PRIDE; Q8R3Q2; -.
DR ProteomicsDB; 289873; -.
DR MGI; MGI:1918724; Ppp6r2.
DR eggNOG; KOG2073; Eukaryota.
DR InParanoid; Q8R3Q2; -.
DR PhylomeDB; Q8R3Q2; -.
DR ChiTaRS; Ppp6r2; mouse.
DR PRO; PR:Q8R3Q2; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q8R3Q2; protein.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0019903; F:protein phosphatase binding; ISA:MGI.
DR GO; GO:0019888; F:protein phosphatase regulator activity; IBA:GO_Central.
DR GO; GO:0043666; P:regulation of phosphoprotein phosphatase activity; ISA:MGI.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR007587; SAPS.
DR PANTHER; PTHR12634; PTHR12634; 1.
DR Pfam; PF04499; SAPS; 2.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..923
FT /note="Serine/threonine-protein phosphatase 6 regulatory
FT subunit 2"
FT /id="PRO_0000046099"
FT REGION 409..441
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 662..685
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 743..766
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 426..441
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 666..685
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 289
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O75170"
FT MOD_RES 746
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 796
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O75170"
FT CONFLICT 106
FT /note="L -> H (in Ref. 2; AK018403)"
FT /evidence="ECO:0000305"
FT CONFLICT 167
FT /note="L -> C (in Ref. 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 195
FT /note="L -> I (in Ref. 2)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 923 AA; 100460 MW; 02F604745D69067B CRC64;
MFWKFDLNTT SHVDKLLDKE HVTLQELMDE DDILQECKAQ NQKLLDFLCR QQCMEELVNL
ITQDPPQDME EKVRFKYPNT ACELLTCDVP QISDRLGEDE SLLNLLYDFL DQEPPLNPLL
ASFFSKTIGN LIARKTEQVI MFLKKKEKFI SQLLKHIGTS ALMDLLLRLV SCVEPVGLRQ
EVLHWLNEEK IIQRLVALIH PHQDEDRQSN ASQALCDIIR LGRDQGSQLQ ETVEPDPLLI
TLESQDCVEQ LLKNMFDGDQ TESCLVSGMQ VLLALLEPRR VGTEGLVDSF SQGLERSHSV
SSSILRGIEP WLKNFHQLLL NPPKKKAILT TIGVLEEPLG NARLHGARLM AALLHTNTPG
INQELCRLNT MDLLLDLFFK YTWNNFLHLQ VELCIAAILS HAAREEQAEA SGSDGKVEPL
QGSGDGNGKL ETTPSITSPP ENTMVTHLFQ KCCLVQRILE AWEANDHTQA AGGMRRGNMG
HLTRIANAVV QNLEQGPVQA HISEVIRGLP ADCRGRWESF VEETLMETNR RNTVDLAFSE
YQIQQMTANF VDQFGFNDEE FADQDDNINA PFDRIAEINF NIEADEDSPS AALFEACCSD
RIQPFDDDEE EDIWEDDETR CAARVMARAR FGAPHVSDNY SKNALEHGGQ DRKTGSAVAR
NVPGLAAPSS PTQKEGPRSE SDSAGTTWTA VFDEPVNPLS ATPGAARDVG SSAWAAGPSV
VEEKGWAKFT DFQPFCCSET GPRCSSPVDM DHSNAEGGQS PGPEKTFGPT SPCAWNVCVT
RKAPLVASDS SSSGGSDSED DEKAAGAVEA VCTGHTGKVS PPPRTAEAAV GRAECPDSTV
LAPACPAPSE VTISPAVATI APSKAGSPTA TIVVSSSVAA AVPPGPIVAV TTAAPAIVAT
LGTMTKDRKA DALPEGAALN GPV
