PPA8_ARATH
ID PPA8_ARATH Reviewed; 335 AA.
AC Q8VYZ2; Q3EC86; Q9SDZ8; Q9SIS5;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Purple acid phosphatase 8;
DE EC=3.1.3.2;
DE Flags: Precursor;
GN Name=PAP8; OrderedLocusNames=At2g01890; ORFNames=T23K3.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Col-1;
RX PubMed=12021284; DOI=10.1074/jbc.m204183200;
RA Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.;
RT "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and
RT differential regulation by phosphate deprivation.";
RL J. Biol. Chem. 277:27772-27781(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING
RP (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-314 (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=10854785; DOI=10.1016/s0378-1119(00)00186-4;
RA Schenk G., Guddat L.W., Ge Y., Carrington L.E., Hume D.A., Hamilton S.,
RA de Jersey J.;
RT "Identification of mammalian-like purple acid phosphatases in a wide range
RT of plants.";
RL Gene 250:117-125(2000).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=16244908; DOI=10.1007/s11103-005-0183-0;
RA Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.;
RT "Expression patterns of purple acid phosphatase genes in Arabidopsis organs
RT and functional analysis of AtPAP23 predominantly transcribed in flower.";
RL Plant Mol. Biol. 59:581-594(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a phosphate monoester + H2O = an alcohol + phosphate;
CC Xref=Rhea:RHEA:15017, ChEBI:CHEBI:15377, ChEBI:CHEBI:30879,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:67140; EC=3.1.3.2;
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
CC Note=Binds 1 Fe cation per subunit. {ECO:0000250};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8VYZ2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VYZ2-2; Sequence=VSP_037190;
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, leaves, flowers and
CC siliques. {ECO:0000269|PubMed:16244908}.
CC -!- SIMILARITY: Belongs to the metallophosphoesterase superfamily. Purple
CC acid phosphatase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD21785.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAF19823.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF492660; AAM15909.1; -; mRNA.
DR EMBL; AC007069; AAD21785.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC05512.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05513.1; -; Genomic_DNA.
DR EMBL; AY065434; AAL38875.1; -; mRNA.
DR EMBL; AY117232; AAM51307.1; -; mRNA.
DR EMBL; AF200827; AAF19823.1; ALT_FRAME; mRNA.
DR PIR; D84430; D84430.
DR RefSeq; NP_178298.2; NM_126250.5. [Q8VYZ2-1]
DR RefSeq; NP_973397.1; NM_201668.2. [Q8VYZ2-2]
DR AlphaFoldDB; Q8VYZ2; -.
DR SMR; Q8VYZ2; -.
DR BioGRID; 123; 1.
DR IntAct; Q8VYZ2; 1.
DR STRING; 3702.AT2G01890.1; -.
DR PaxDb; Q8VYZ2; -.
DR PRIDE; Q8VYZ2; -.
DR ProteomicsDB; 249117; -. [Q8VYZ2-1]
DR EnsemblPlants; AT2G01890.1; AT2G01890.1; AT2G01890. [Q8VYZ2-1]
DR EnsemblPlants; AT2G01890.2; AT2G01890.2; AT2G01890. [Q8VYZ2-2]
DR GeneID; 814720; -.
DR Gramene; AT2G01890.1; AT2G01890.1; AT2G01890. [Q8VYZ2-1]
DR Gramene; AT2G01890.2; AT2G01890.2; AT2G01890. [Q8VYZ2-2]
DR KEGG; ath:AT2G01890; -.
DR Araport; AT2G01890; -.
DR TAIR; locus:2059748; AT2G01890.
DR eggNOG; KOG2679; Eukaryota.
DR InParanoid; Q8VYZ2; -.
DR OMA; CTHHPYL; -.
DR OrthoDB; 711825at2759; -.
DR PhylomeDB; Q8VYZ2; -.
DR BioCyc; ARA:AT2G01890-MON; -.
DR PRO; PR:Q8VYZ2; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8VYZ2; baseline and differential.
DR Genevisible; Q8VYZ2; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0003993; F:acid phosphatase activity; ISS:TAIR.
DR GO; GO:0008199; F:ferric iron binding; IBA:GO_Central.
DR GO; GO:0008198; F:ferrous iron binding; IBA:GO_Central.
DR GO; GO:0016311; P:dephosphorylation; ISS:TAIR.
DR CDD; cd07378; MPP_ACP5; 1.
DR Gene3D; 3.60.21.10; -; 1.
DR InterPro; IPR024927; Acid_PPase.
DR InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR InterPro; IPR029052; Metallo-depent_PP-like.
DR Pfam; PF00149; Metallophos; 1.
DR PIRSF; PIRSF000898; Acid_Ptase_5; 1.
DR SUPFAM; SSF56300; SSF56300; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Hydrolase; Iron; Metal-binding;
KW Reference proteome; Secreted; Signal; Zinc.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..335
FT /note="Purple acid phosphatase 8"
FT /id="PRO_0000372813"
FT ACT_SITE 226
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 52
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250"
FT BINDING 85
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250"
FT BINDING 85
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 88
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250"
FT BINDING 123
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 217
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 252..254
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 252
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 254
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000250"
FT CARBOHYD 60
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 128..155
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_037190"
FT CONFLICT 261
FT /note="S -> G (in Ref. 5; AAF19823)"
FT /evidence="ECO:0000305"
FT CONFLICT 266
FT /note="I -> M (in Ref. 5; AAF19823)"
FT /evidence="ECO:0000305"
FT CONFLICT 275
FT /note="S -> F (in Ref. 5; AAF19823)"
FT /evidence="ECO:0000305"
FT CONFLICT 287
FT /note="P -> L (in Ref. 5; AAF19823)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 335 AA; 38173 MW; 4202286D73CBFF7C CRC64;
MDSLRDVKPI KLIFSIFCLV IILSACNSTA ELPRFVQPPE PDGSLSFLVV GDWGRRGSYN
QSQVALQMGK IGKDLNIDFL ISTGDNFYDD GIISPYDSQF QDSFTNIYTA TSLQKPWYNV
LGNHDYRGNV YAQLSPILRD LDCRWICLRS YVVNAEIVDI FFVDTTPFVD RYFDEPKDHV
YDWRGVLPRN KYLNSLLTDV DVALQESMAK WKIVVGHHTI KSAGHHGNTI ELEKQLLPIL
EANEVDLYIN GHDHCLEHIS SINSGIQFMT SGGGSKAWKG DVNDWNPQEM RFYYDGQGFM
SVYTSEAELR VVFYDGLGHV LHRWSTLKNG VYSDI
