PPAC_METJA
ID PPAC_METJA Reviewed; 307 AA.
AC Q58025;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Manganese-dependent inorganic pyrophosphatase;
DE EC=3.6.1.1;
DE AltName: Full=Pyrophosphate phospho-hydrolase;
DE Short=PPase;
GN Name=ppaC; OrderedLocusNames=MJ0608;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
RN [2]
RP PROTEIN SEQUENCE OF 1-5, CATALYTIC ACTIVITY, AND MASS SPECTROMETRY.
RX PubMed=10898947; DOI=10.1006/abbi.2000.1860;
RA Kuhn N.J., Wadeson A., Ward S., Young T.W.;
RT "Methanococcus jannaschii ORF mj0608 codes for a class C inorganic
RT pyrophosphatase protected by Co(2+) or Mn(2+) ions against fluoride
RT inhibition.";
RL Arch. Biochem. Biophys. 379:292-298(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=diphosphate + H2O = H(+) + 2 phosphate; Xref=Rhea:RHEA:24576,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:43474; EC=3.6.1.1;
CC Evidence={ECO:0000269|PubMed:10898947};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Note=Binds 2 manganese ions per subunit. {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- MASS SPECTROMETRY: Mass=34169; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:10898947};
CC -!- SIMILARITY: Belongs to the PPase class C family. {ECO:0000305}.
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DR EMBL; L77117; AAB98601.1; -; Genomic_DNA.
DR PIR; H64375; H64375.
DR RefSeq; WP_010870112.1; NC_000909.1.
DR PDB; 2EB0; X-ray; 2.20 A; A/B=1-307.
DR PDBsum; 2EB0; -.
DR AlphaFoldDB; Q58025; -.
DR SMR; Q58025; -.
DR STRING; 243232.MJ_0608; -.
DR EnsemblBacteria; AAB98601; AAB98601; MJ_0608.
DR GeneID; 1451473; -.
DR KEGG; mja:MJ_0608; -.
DR eggNOG; arCOG01567; Archaea.
DR HOGENOM; CLU_025243_0_1_2; -.
DR InParanoid; Q58025; -.
DR OMA; IMLCAIL; -.
DR OrthoDB; 57518at2157; -.
DR PhylomeDB; Q58025; -.
DR EvolutionaryTrace; Q58025; -.
DR Proteomes; UP000000805; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0004427; F:inorganic diphosphatase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-UniRule.
DR Gene3D; 3.10.310.20; -; 1.
DR HAMAP; MF_00207; PPase_C; 1.
DR InterPro; IPR001667; DDH_dom.
DR InterPro; IPR038763; DHH_sf.
DR InterPro; IPR004097; DHHA2.
DR InterPro; IPR038222; DHHA2_dom_sf.
DR InterPro; IPR022934; Mn-dep_inorganic_PyrPase.
DR Pfam; PF01368; DHH; 1.
DR Pfam; PF02833; DHHA2; 1.
DR SMART; SM01131; DHHA2; 1.
DR SUPFAM; SSF64182; SSF64182; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cobalt; Cytoplasm; Direct protein sequencing; Hydrolase;
KW Manganese; Metal-binding; Reference proteome.
FT CHAIN 1..307
FT /note="Manganese-dependent inorganic pyrophosphatase"
FT /id="PRO_0000158599"
FT BINDING 7
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 11
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 13
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 66
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 66
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 88
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 147
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT STRAND 2..5
FT /evidence="ECO:0007829|PDB:2EB0"
FT HELIX 12..25
FT /evidence="ECO:0007829|PDB:2EB0"
FT STRAND 27..33
FT /evidence="ECO:0007829|PDB:2EB0"
FT HELIX 37..46
FT /evidence="ECO:0007829|PDB:2EB0"
FT STRAND 61..66
FT /evidence="ECO:0007829|PDB:2EB0"
FT HELIX 70..72
FT /evidence="ECO:0007829|PDB:2EB0"
FT HELIX 77..79
FT /evidence="ECO:0007829|PDB:2EB0"
FT STRAND 80..89
FT /evidence="ECO:0007829|PDB:2EB0"
FT STRAND 100..103
FT /evidence="ECO:0007829|PDB:2EB0"
FT STRAND 105..107
FT /evidence="ECO:0007829|PDB:2EB0"
FT HELIX 109..118
FT /evidence="ECO:0007829|PDB:2EB0"
FT HELIX 122..125
FT /evidence="ECO:0007829|PDB:2EB0"
FT HELIX 134..148
FT /evidence="ECO:0007829|PDB:2EB0"
FT TURN 149..152
FT /evidence="ECO:0007829|PDB:2EB0"
FT HELIX 158..171
FT /evidence="ECO:0007829|PDB:2EB0"
FT HELIX 176..188
FT /evidence="ECO:0007829|PDB:2EB0"
FT HELIX 189..192
FT /evidence="ECO:0007829|PDB:2EB0"
FT HELIX 195..199
FT /evidence="ECO:0007829|PDB:2EB0"
FT STRAND 202..208
FT /evidence="ECO:0007829|PDB:2EB0"
FT STRAND 211..221
FT /evidence="ECO:0007829|PDB:2EB0"
FT HELIX 224..243
FT /evidence="ECO:0007829|PDB:2EB0"
FT STRAND 247..255
FT /evidence="ECO:0007829|PDB:2EB0"
FT TURN 256..259
FT /evidence="ECO:0007829|PDB:2EB0"
FT STRAND 260..267
FT /evidence="ECO:0007829|PDB:2EB0"
FT HELIX 269..275
FT /evidence="ECO:0007829|PDB:2EB0"
FT STRAND 282..287
FT /evidence="ECO:0007829|PDB:2EB0"
FT HELIX 293..296
FT /evidence="ECO:0007829|PDB:2EB0"
FT HELIX 298..305
FT /evidence="ECO:0007829|PDB:2EB0"
SQ SEQUENCE 307 AA; 34113 MW; EF7EDD1610668D9A CRC64;
MRYVVGHKNP DTDSIASAIV LAYFLDCYPA RLGDINPETE FVLRKFGVME PELIESAKGK
EIILVDHSEK SQSFDDLEEG KLIAIIDHHK VGLTTTEPIL YYAKPVGSTA TVIAELYFKD
AIDLIGGKKK ELKPDLAGLL LSAIISDTVL FKSPTTTDLD KEMAKKLAEI AGISNIEEFG
MEILKAKSVV GKLKPEEIIN MDFKNFDFNG KKVGIGQVEV IDVSEVESKK EDIYKLLEEK
LKNEGYDLIV FLITDIMKEG SEALVVGNKE MFEKAFNVKV EGNSVFLEGV MSRKKQVVPP
LERAYNG
