ID   PPAC_PRIMG              Reviewed;          16 AA.
AC   P56948;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=Manganese-dependent inorganic pyrophosphatase;
DE            EC=3.6.1.1;
DE   AltName: Full=Pyrophosphate phospho-hydrolase;
DE            Short=PPase;
DE   Flags: Fragment;
GN   Name=ppaC;
OS   Priestia megaterium (Bacillus megaterium).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Priestia.
OX   NCBI_TaxID=1404;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=9782505; DOI=10.1099/00221287-144-9-2563;
RA   Young T.W., Kuhn N.J., Wadeson A., Ward S., Burges D., Cooke G.D.;
RT   "Bacillus subtilis ORF yybQ encodes a manganese-dependent inorganic
RT   pyrophosphatase with distinctive properties: the first of a new class of
RT   soluble pyrophosphatase?";
RL   Microbiology 144:2563-2571(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=diphosphate + H2O = H(+) + 2 phosphate; Xref=Rhea:RHEA:24576,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:43474; EC=3.6.1.1;
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 2 manganese ions per subunit. {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PPase class C family. {ECO:0000305}.
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DR   AlphaFoldDB; P56948; -.
DR   STRING; 1348908.KI518643_gene187; -.
DR   eggNOG; COG1227; Bacteria.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004427; F:inorganic diphosphatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Hydrolase; Manganese; Metal-binding.
FT   CHAIN           1..>16
FT                   /note="Manganese-dependent inorganic pyrophosphatase"
FT                   /id="PRO_0000158569"
FT   BINDING         8
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         12
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         14
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   NON_TER         16
SQ   SEQUENCE   16 AA;  1828 MW;  3C0E6735D98B38A0 CRC64;
     QKTLIFGHKN PDTDTI