PPAC_STAAM
ID PPAC_STAAM Reviewed; 309 AA.
AC P65752; Q99SW8;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Probable manganese-dependent inorganic pyrophosphatase {ECO:0000255|HAMAP-Rule:MF_00207};
DE EC=3.6.1.1 {ECO:0000255|HAMAP-Rule:MF_00207};
DE AltName: Full=Pyrophosphate phospho-hydrolase {ECO:0000255|HAMAP-Rule:MF_00207};
DE Short=PPase {ECO:0000255|HAMAP-Rule:MF_00207};
GN Name=ppaC {ECO:0000255|HAMAP-Rule:MF_00207}; OrderedLocusNames=SAV1919;
OS Staphylococcus aureus (strain Mu50 / ATCC 700699).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=158878;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Mu50 / ATCC 700699;
RX PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA Hiramatsu K.;
RT "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL Lancet 357:1225-1240(2001).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=diphosphate + H2O = H(+) + 2 phosphate; Xref=Rhea:RHEA:24576,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:43474; EC=3.6.1.1; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00207};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00207};
CC Note=Binds 2 manganese ions per subunit. {ECO:0000255|HAMAP-
CC Rule:MF_00207};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00207}.
CC -!- SIMILARITY: Belongs to the PPase class C family. {ECO:0000255|HAMAP-
CC Rule:MF_00207}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BA000017; BAB58081.1; -; Genomic_DNA.
DR RefSeq; WP_001140871.1; NC_002758.2.
DR PDB; 4RPA; X-ray; 2.10 A; A/B=1-309.
DR PDBsum; 4RPA; -.
DR AlphaFoldDB; P65752; -.
DR SMR; P65752; -.
DR World-2DPAGE; 0002:P65752; -.
DR PaxDb; P65752; -.
DR EnsemblBacteria; BAB58081; BAB58081; SAV1919.
DR KEGG; sav:SAV1919; -.
DR HOGENOM; CLU_025243_0_1_9; -.
DR OMA; IMLCAIL; -.
DR PhylomeDB; P65752; -.
DR BioCyc; SAUR158878:SAV_RS10515-MON; -.
DR BRENDA; 3.6.1.1; 3352.
DR Proteomes; UP000002481; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004427; F:inorganic diphosphatase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-UniRule.
DR Gene3D; 3.10.310.20; -; 1.
DR HAMAP; MF_00207; PPase_C; 1.
DR InterPro; IPR001667; DDH_dom.
DR InterPro; IPR038763; DHH_sf.
DR InterPro; IPR004097; DHHA2.
DR InterPro; IPR038222; DHHA2_dom_sf.
DR InterPro; IPR022934; Mn-dep_inorganic_PyrPase.
DR Pfam; PF01368; DHH; 1.
DR Pfam; PF02833; DHHA2; 1.
DR SMART; SM01131; DHHA2; 1.
DR SUPFAM; SSF64182; SSF64182; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Hydrolase; Manganese; Metal-binding.
FT CHAIN 1..309
FT /note="Probable manganese-dependent inorganic
FT pyrophosphatase"
FT /id="PRO_0000158580"
FT BINDING 9
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00207"
FT BINDING 13
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00207"
FT BINDING 15
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00207"
FT BINDING 75
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00207"
FT BINDING 75
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00207"
FT BINDING 97
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00207"
FT BINDING 149
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00207"
FT STRAND 4..7
FT /evidence="ECO:0007829|PDB:4RPA"
FT HELIX 14..29
FT /evidence="ECO:0007829|PDB:4RPA"
FT STRAND 35..41
FT /evidence="ECO:0007829|PDB:4RPA"
FT HELIX 45..53
FT /evidence="ECO:0007829|PDB:4RPA"
FT STRAND 70..75
FT /evidence="ECO:0007829|PDB:4RPA"
FT HELIX 79..81
FT /evidence="ECO:0007829|PDB:4RPA"
FT HELIX 86..88
FT /evidence="ECO:0007829|PDB:4RPA"
FT STRAND 89..96
FT /evidence="ECO:0007829|PDB:4RPA"
FT STRAND 110..113
FT /evidence="ECO:0007829|PDB:4RPA"
FT STRAND 115..117
FT /evidence="ECO:0007829|PDB:4RPA"
FT HELIX 119..130
FT /evidence="ECO:0007829|PDB:4RPA"
FT HELIX 136..150
FT /evidence="ECO:0007829|PDB:4RPA"
FT TURN 151..154
FT /evidence="ECO:0007829|PDB:4RPA"
FT HELIX 160..173
FT /evidence="ECO:0007829|PDB:4RPA"
FT HELIX 177..187
FT /evidence="ECO:0007829|PDB:4RPA"
FT HELIX 196..201
FT /evidence="ECO:0007829|PDB:4RPA"
FT STRAND 204..209
FT /evidence="ECO:0007829|PDB:4RPA"
FT STRAND 212..223
FT /evidence="ECO:0007829|PDB:4RPA"
FT HELIX 224..228
FT /evidence="ECO:0007829|PDB:4RPA"
FT HELIX 231..245
FT /evidence="ECO:0007829|PDB:4RPA"
FT STRAND 248..256
FT /evidence="ECO:0007829|PDB:4RPA"
FT TURN 257..259
FT /evidence="ECO:0007829|PDB:4RPA"
FT STRAND 262..268
FT /evidence="ECO:0007829|PDB:4RPA"
FT HELIX 271..275
FT /evidence="ECO:0007829|PDB:4RPA"
FT STRAND 280..283
FT /evidence="ECO:0007829|PDB:4RPA"
FT STRAND 285..292
FT /evidence="ECO:0007829|PDB:4RPA"
FT STRAND 295..297
FT /evidence="ECO:0007829|PDB:4RPA"
FT HELIX 299..308
FT /evidence="ECO:0007829|PDB:4RPA"
SQ SEQUENCE 309 AA; 34069 MW; 39EB877DA5E485A2 CRC64;
MAKTYIFGHK NPDTDAISSA IIMAEFEQLR GNSGAKAYRL GDVSAETQFA LDTFNVPAPE
LLTDDLDGQD VILVDHNEFQ QSSDTIASAT IKHVIDHHRI ANFETAGPLC YRAEPVGCTA
TILYKMFRER GFEIKPEIAG LMLSAIISDS LLFKSPTCTQ QDVKAAEELK DIAKVDIQKY
GLDMLKAGAS TTDKSVEFLL NMDAKSFTMG DYVTRIAQVN AVDLDEVLNR KEDLEKEMLA
VSAQEKYDLF VLVVTDIINS DSKILVVGAE KDKVGEAFNV QLEDDMAFLS GVVSRKKQIV
PQITEALTK
