PPAT_MOUSE
ID PPAT_MOUSE Reviewed; 425 AA.
AC D3YTS9;
DT 10-MAY-2017, integrated into UniProtKB/Swiss-Prot.
DT 17-FEB-2016, sequence version 2.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Testicular acid phosphatase {ECO:0000305};
DE EC=3.1.3.2;
DE AltName: Full=Acid phosphatase 4 {ECO:0000312|MGI:MGI:3644563};
DE Flags: Precursor;
GN Name=Acp4 {ECO:0000312|MGI:MGI:3644563};
GN Synonyms=Acpt {ECO:0000312|MGI:MGI:3644563};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP DEVELOPMENTAL STAGE.
RX PubMed=27843125; DOI=10.1016/j.ajhg.2016.09.018;
RA Seymen F., Kim Y.J., Lee Y.J., Kang J., Kim T.H., Choi H., Koruyucu M.,
RA Kasimoglu Y., Tuna E.B., Gencay K., Shin T.J., Hyun H.K., Kim Y.J.,
RA Lee S.H., Lee Z.H., Zhang H., Hu J.C., Simmer J.P., Cho E.S., Kim J.W.;
RT "Recessive mutations in ACPT, encoding testicular acid phosphatase, cause
RT hypoplastic amelogenesis imperfecta.";
RL Am. J. Hum. Genet. 99:1199-1205(2016).
CC -!- FUNCTION: May dephosphorylate receptor tyrosine-protein kinase ERBB4
CC and inhibits its ligand-induced proteolytic cleavage. May play a role
CC in odontogenesis. {ECO:0000250|UniProtKB:Q9BZG2}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a phosphate monoester + H2O = an alcohol + phosphate;
CC Xref=Rhea:RHEA:15017, ChEBI:CHEBI:15377, ChEBI:CHEBI:30879,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:67140; EC=3.1.3.2;
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:Q9BZG2}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- DEVELOPMENTAL STAGE: In the developing tooth from 8-day-old mice it is
CC expressed in secretory-stage ameloblasts, follicular cells,
CC odontoblasts, and osteoblasts (at protein level).
CC {ECO:0000269|PubMed:27843125}.
CC -!- PTM: Glycosylated. {ECO:0000250|UniProtKB:Q9BZG2}.
CC -!- SIMILARITY: Belongs to the histidine acid phosphatase family.
CC {ECO:0000305}.
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DR EMBL; AC152939; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS57546.1; -.
DR RefSeq; NP_001181963.1; NM_001195034.1.
DR AlphaFoldDB; D3YTS9; -.
DR SMR; D3YTS9; -.
DR STRING; 10090.ENSMUSP00000103578; -.
DR iPTMnet; D3YTS9; -.
DR PhosphoSitePlus; D3YTS9; -.
DR PaxDb; D3YTS9; -.
DR PRIDE; D3YTS9; -.
DR Antibodypedia; 32366; 81 antibodies from 20 providers.
DR Ensembl; ENSMUST00000118216; ENSMUSP00000112922; ENSMUSG00000012777.
DR GeneID; 100503991; -.
DR KEGG; mmu:100503991; -.
DR CTD; 93650; -.
DR MGI; MGI:3644563; Acp4.
DR VEuPathDB; HostDB:ENSMUSG00000012777; -.
DR eggNOG; KOG3720; Eukaryota.
DR GeneTree; ENSGT00940000161433; -.
DR HOGENOM; CLU_030431_1_1_1; -.
DR OMA; PCHGSRE; -.
DR OrthoDB; 1221585at2759; -.
DR BioGRID-ORCS; 100503991; 5 hits in 71 CRISPR screens.
DR PRO; PR:D3YTS9; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; D3YTS9; protein.
DR Bgee; ENSMUSG00000012777; Expressed in testis and 26 other tissues.
DR ExpressionAtlas; D3YTS9; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005764; C:lysosome; IBA:GO_Central.
DR GO; GO:0045211; C:postsynaptic membrane; ISO:MGI.
DR GO; GO:0003993; F:acid phosphatase activity; IBA:GO_Central.
DR GO; GO:0016791; F:phosphatase activity; IBA:GO_Central.
DR GO; GO:0004725; F:protein tyrosine phosphatase activity; ISS:UniProtKB.
DR GO; GO:0030971; F:receptor tyrosine kinase binding; ISS:UniProtKB.
DR GO; GO:0016311; P:dephosphorylation; IBA:GO_Central.
DR GO; GO:0007040; P:lysosome organization; IBA:GO_Central.
DR GO; GO:0120154; P:negative regulation of ERBB4 signaling pathway; ISS:UniProtKB.
DR GO; GO:0010977; P:negative regulation of neuron projection development; ISS:UniProtKB.
DR GO; GO:0010955; P:negative regulation of protein processing; ISS:UniProtKB.
DR GO; GO:0042476; P:odontogenesis; ISS:UniProtKB.
DR GO; GO:1990264; P:peptidyl-tyrosine dephosphorylation involved in inactivation of protein kinase activity; ISS:UniProtKB.
DR GO; GO:0048168; P:regulation of neuronal synaptic plasticity; ISS:UniProtKB.
DR CDD; cd07061; HP_HAP_like; 1.
DR Gene3D; 3.40.50.1240; -; 1.
DR InterPro; IPR033379; Acid_Pase_AS.
DR InterPro; IPR000560; His_Pase_clade-2.
DR InterPro; IPR029033; His_PPase_superfam.
DR Pfam; PF00328; His_Phos_2; 1.
DR SUPFAM; SSF53254; SSF53254; 1.
DR PROSITE; PS00616; HIS_ACID_PHOSPHAT_1; 1.
DR PROSITE; PS00778; HIS_ACID_PHOSPHAT_2; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Glycoprotein; Hydrolase; Membrane; Reference proteome;
KW Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..425
FT /note="Testicular acid phosphatase"
FT /id="PRO_5006722351"
FT TOPO_DOM 28..392
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 393..413
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 414..425
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT ACT_SITE 40
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:P15309"
FT ACT_SITE 288
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:P15309"
FT DISULFID 158..378
FT /evidence="ECO:0000250|UniProtKB:P15309"
FT DISULFID 213..311
FT /evidence="ECO:0000250|UniProtKB:P15309"
FT DISULFID 353..357
FT /evidence="ECO:0000250|UniProtKB:P15309"
SQ SEQUENCE 425 AA; 46405 MW; 487B981362A49E97 CRC64;
MAEPGSQGHT VGPLLLLLLL LLPRALPEGP LLFVALVFRH GDRAPLASYP TDPHKEAAST
LWPRGLGQLT KEGIRQQLEL GRFLRRRYKA FLSPEYKREE VYIRSTDFDR TLESAQANLA
GLFPEAAPGS PETDWKPIPV HTVPVSEDKL LRFPMRSCPR YHELLRESTE AADYQEALEG
WTDFLTRLGN FTGLSLVGEP LRRAWKVLDT LICQRAHGLD LPSWASPDVL RTLSQISALD
IRAHVGPPRA AEKAQLTGGI LLDAILSNFS RTQRLGLPLK MVMYSAHDST LLALQGALGL
YDGNTPPYAA CMAFEFRGSS REPEEEDGEN VTVSLIYRND TSRPPLPLRV PGCPAPCPLG
RFQQLTAPAR PPAHGAPCHG SYEPASPPAT VPLLAGAVAV LAVLSLGLGL LAWRPRCLRA
LGGTV
