ID   PPA_SCHCO               Reviewed;          10 AA.
AC   B3EWC6;
DT   25-JAN-2012, integrated into UniProtKB/Swiss-Prot.
DT   25-JAN-2012, sequence version 1.
DT   02-JUN-2021, entry version 11.
DE   RecName: Full=Acid phosphatase {ECO:0000303|PubMed:23322529};
DE            EC=3.1.3.2 {ECO:0000269|PubMed:23322529};
DE   Flags: Fragment;
OS   Schizophyllum commune (Split gill fungus).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Schizophyllaceae; Schizophyllum.
OX   NCBI_TaxID=5334;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, CATALYTIC ACTIVITY, ACTIVITY REGULATION,
RP   BIOPHYSICOCHEMICAL PROPERTIES, AND SUBUNIT.
RC   STRAIN=0805 {ECO:0000269|PubMed:23322529};
RC   TISSUE=Fruiting body {ECO:0000269|PubMed:23322529};
RX   PubMed=23322529; DOI=10.1002/jobm.201200218;
RA   Zhang G.Q., Chen Q.J., Sun J., Wang H.X., Han C.H.;
RT   "Purification and characterization of a novel acid phosphatase from the
RT   split gill mushroom Schizophyllum commune.";
RL   J. Basic Microbiol. 53:868-875(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a phosphate monoester + H2O = an alcohol + phosphate;
CC         Xref=Rhea:RHEA:15017, ChEBI:CHEBI:15377, ChEBI:CHEBI:30879,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:67140; EC=3.1.3.2;
CC         Evidence={ECO:0000269|PubMed:23322529};
CC   -!- ACTIVITY REGULATION: Activated slightly by Co(2+), Ca(2+) and Mg(2+).
CC       Strongly inhibited by NaF, Fe(3+) and Al(3+), less so by tartrate,
CC       Cu(2+) and Hg(+). Not affected by EDTA, Fe(2+) and Zn(2+).
CC       {ECO:0000269|PubMed:23322529}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=248 uM for para-nitrophenylphosphate (p-NPP)
CC         {ECO:0000269|PubMed:23322529};
CC         Vmax=9 nM/min/mg enzyme {ECO:0000269|PubMed:23322529};
CC       pH dependence:
CC         Optimum pH is 4.6. Activity decreases sharply with decreases and
CC         increases in pH and is absent at pH 8.0. When pre-incubated at
CC         different pH levels, activity is stable between pH 4 and 9.
CC         {ECO:0000269|PubMed:23322529};
CC       Temperature dependence:
CC         Optimum temperature is 50 degrees Celsius. Activity decreases sharply
CC         with decreases and increases in temperature. When pre-incubated at
CC         different temperatures, activity is stable between 20 and 50 degrees
CC         Celsius. {ECO:0000269|PubMed:23322529};
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:23322529}.
CC   -!- MISCELLANEOUS: Has no activity towards sodium phytate, ATP, ADP, AMP,
CC       glucose-6-phosphate, fructose-6-phosphate and beta-glycerophosphate.
CC       {ECO:0000269|PubMed:23322529}.
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DR   BRENDA; 3.1.3.2; 5611.
DR   GO; GO:0003993; F:acid phosphatase activity; IDA:UniProtKB.
DR   GO; GO:0016311; P:dephosphorylation; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase.
FT   CHAIN           1..>10
FT                   /note="Acid phosphatase"
FT                   /id="PRO_0000415411"
FT   NON_TER         10
FT                   /evidence="ECO:0000303|PubMed:23322529"
SQ   SEQUENCE   10 AA;  1142 MW;  2292ECA046DDC367 CRC64;
     NAPWAQIDEV