ID   PPBL_PSEAB              Reviewed;         392 AA.
AC   Q02HI0;
DT   07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   03-AUG-2022, entry version 54.
DE   RecName: Full=Alkaline phosphatase L;
DE            Short=L-AP;
DE            EC=3.1.3.1 {ECO:0000250|UniProtKB:P35482};
DE   AltName: Full=Low molecular weight phosphatase;
DE   AltName: Full=Protein DING {ECO:0000303|PubMed:24372739};
DE   Flags: Precursor;
GN   Name=phoA2 {ECO:0000305}; Synonyms=dinG {ECO:0000303|PubMed:24372739};
GN   OrderedLocusNames=PA14_55410;
OS   Pseudomonas aeruginosa (strain UCBPP-PA14).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UCBPP-PA14;
RX   PubMed=17038190; DOI=10.1186/gb-2006-7-10-r90;
RA   Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S.,
RA   Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L., Grills G.,
RA   Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M.;
RT   "Genomic analysis reveals that Pseudomonas aeruginosa virulence is
RT   combinatorial.";
RL   Genome Biol. 7:R90.1-R90.14(2006).
RN   [2]
RP   SUBCELLULAR LOCATION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=UCBPP-PA14;
RX   PubMed=24372739; DOI=10.1111/1574-6968.12368;
RA   Shah M., Zaborin A., Alverdy J.C., Scott K., Zaborina O.;
RT   "Localization of DING proteins on PstS-containing outer-surface appendages
RT   of Pseudomonas aeruginosa.";
RL   FEMS Microbiol. Lett. 352:54-61(2014).
CC   -!- FUNCTION: Has both a phosphomonoesterase and phosphodiesterase
CC       activity. {ECO:0000250|UniProtKB:P35482}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a phosphate monoester + H2O = an alcohol + phosphate;
CC         Xref=Rhea:RHEA:15017, ChEBI:CHEBI:15377, ChEBI:CHEBI:30879,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:67140; EC=3.1.3.1;
CC         Evidence={ECO:0000250|UniProtKB:P35482};
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P35482}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:24372739}. Periplasm
CC       {ECO:0000250|UniProtKB:P35482}. Note=Forms long appendages distinct
CC       from flagella or pili on the cell surface in approximately 1% of cells.
CC       When overexpressed more cells form more appendages.
CC       {ECO:0000269|PubMed:24372739}.
CC   -!- INDUCTION: Suppressed by inorganic phosphate.
CC       {ECO:0000269|PubMed:24372739}.
CC   -!- DISRUPTION PHENOTYPE: Strains are still able to make extracellular
CC       appendages that include PstS. {ECO:0000269|PubMed:24372739}.
CC   -!- SIMILARITY: Belongs to the PstS family. {ECO:0000305}.
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DR   EMBL; CP000438; ABJ09831.1; -; Genomic_DNA.
DR   RefSeq; WP_003141140.1; NC_008463.1.
DR   AlphaFoldDB; Q02HI0; -.
DR   SMR; Q02HI0; -.
DR   PRIDE; Q02HI0; -.
DR   EnsemblBacteria; ABJ09831; ABJ09831; PA14_55410.
DR   KEGG; pau:PA14_55410; -.
DR   HOGENOM; CLU_047926_0_0_6; -.
DR   OMA; GRITYMS; -.
DR   BioCyc; PAER208963:G1G74-4667-MON; -.
DR   Proteomes; UP000000653; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0004035; F:alkaline phosphatase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR024370; PBP_domain.
DR   Pfam; PF12849; PBP_like_2; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Periplasm; Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..392
FT                   /note="Alkaline phosphatase L"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000431610"
SQ   SEQUENCE   392 AA;  40714 MW;  1F055BF2387A2182 CRC64;
     MYKRSLIAAS LSVAALVSAQ AMADINGGGA TLPQQLYQEP GVLTAGFAAY IGVGSGNGKA
     AFLNNDYTKF VAGTTNKNVH WAGSDSKLSK TNETNPYLSA HGSAWGPLIQ VPSVATSVAL
     PFNKSGSNAV NFADVNTLCG VFSGRLTDWS QIPGSGRSGA ITVVYRSESS GTTELFTRFL
     NASCSSTLEG GTFAITTSFG SSFSGGLPAG AVSAQGSQAV MNALNAAQGR ITYMSPDFAA
     PTLAGLDDAT KVAQVRGVSP APANVSAAIG AVTPPTTAQR SDPNNWVPVF AATANPNDPS
     VRPYPTSGYP ILGFTNLIFS QCYANATQTQ QVRDFFTRHY GATANNDTAI TNHRFVPLPA
     SWKLAVRQSF LTSTNNLYIG HSNVCNGIGR PL