PPC89_SCHPO
ID PPC89_SCHPO Reviewed; 783 AA.
AC Q10218;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Spindle pole body protein ppc89;
DE AltName: Full=Meiotically up-regulated gene 127 protein;
GN Name=ppc89; Synonyms=mug127; ORFNames=SPAC4H3.11c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION IN MEIOSIS.
RX PubMed=16303567; DOI=10.1016/j.cub.2005.10.038;
RA Martin-Castellanos C., Blanco M., Rozalen A.E., Perez-Hidalgo L.,
RA Garcia A.I., Conde F., Mata J., Ellermeier C., Davis L., San-Segundo P.,
RA Smith G.R., Moreno S.;
RT "A large-scale screen in S. pombe identifies seven novel genes required for
RT critical meiotic events.";
RL Curr. Biol. 15:2056-2062(2005).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-157, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: Has a role in meiosis. {ECO:0000269|PubMed:16303567}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}.
CC Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole
CC body {ECO:0000269|PubMed:16823372}.
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DR EMBL; CU329670; CAA93350.1; -; Genomic_DNA.
DR PIR; T38891; T38891.
DR RefSeq; NP_594347.1; NM_001019768.2.
DR AlphaFoldDB; Q10218; -.
DR SMR; Q10218; -.
DR BioGRID; 279971; 11.
DR IntAct; Q10218; 2.
DR STRING; 4896.SPAC4H3.11c.1; -.
DR iPTMnet; Q10218; -.
DR MaxQB; Q10218; -.
DR PaxDb; Q10218; -.
DR PRIDE; Q10218; -.
DR EnsemblFungi; SPAC4H3.11c.1; SPAC4H3.11c.1:pep; SPAC4H3.11c.
DR GeneID; 2543554; -.
DR KEGG; spo:SPAC4H3.11c; -.
DR PomBase; SPAC4H3.11c; ppc89.
DR VEuPathDB; FungiDB:SPAC4H3.11c; -.
DR eggNOG; ENOG502S7ZB; Eukaryota.
DR HOGENOM; CLU_334364_0_0_1; -.
DR InParanoid; Q10218; -.
DR OMA; INCNAVH; -.
DR PRO; PR:Q10218; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0061493; C:central plaque of mitotic spindle pole body; IDA:PomBase.
DR GO; GO:0005737; C:cytoplasm; HDA:PomBase.
DR GO; GO:0044732; C:mitotic spindle pole body; IDA:PomBase.
DR GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR GO; GO:0140475; F:spindle pole body anchor activity; EXP:PomBase.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0007052; P:mitotic spindle organization; EXP:PomBase.
DR InterPro; IPR024957; Cep57_MT-bd_dom.
DR InterPro; IPR025925; PPC89_CLD.
DR Pfam; PF14197; Cep57_CLD_2; 1.
DR Pfam; PF06657; Cep57_MT_bd; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Meiosis; Phosphoprotein; Reference proteome.
FT CHAIN 1..783
FT /note="Spindle pole body protein ppc89"
FT /id="PRO_0000116485"
FT REGION 180..216
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 434..458
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 471..504
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 528..610
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 197..216
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 534..549
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 570..584
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 585..599
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 157
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
SQ SEQUENCE 783 AA; 89188 MW; FA2FA539AE5682FE CRC64;
MPSQFNDNFV ATDDDVGTMA RVGMALDREL NGDSFQDNLN FQPRSGKRED FEPFFQDTPN
TKSLSKHFHD FTMNASLETL PSVEKPRGRN MNAFEETSWN RFRKNGSLFP LSSPPISEPD
LRPQALNETP DYRNRFLGAF KKQGVLDDHG NLKLDASPSF LKKPAEYTPL ANRQNQNLAF
DSPTEALPPK PTTPWRRNGF RSKTTPNLNS GKETPSSYKA SARLMEQLGL NHSEPSVDFN
NQTSYRLPNL TNLSSLIRDD TIDENGNAKE HDRLPELNTI PVASTDEQLF NAHQLLEKKF
EILKRERNEC NAKIDELQDK LELLTDAYNR EKRRARSLEE RMSKEMLTKL GESNVDDGMA
ASRYDTVKRE KERLSEHLKS LQEQYEHIQS VYKNVLLDRE SYIMRLGNKI SENNELLNEN
RVLKEKLQTY LDKKESNVTS KIKSTAENSS KPLSMNEADE RKDGLNNLLF ENKSGANTKE
MSNGTETAKE NCSPQQDSTS PTSGYQDLVK ELAKEIEMRK SLELKLKLSQ SNKAGPVKHR
KRRPKSKRRI TGKVVFDSPN VASGVESDEG SEEISLDSEY SDILSDDGDF EKEKQATLPR
RRSSSSMKGN KLAEDSYLNE AGFDWNQGTF HNGSEFGTTG VPDEPNEEEL PKHVLKQVEH
IINESAAHGV GKCNACHARQ EDLIRGEQKV SHSNCLYADQ TLRPSQPPSE ALKTVVNQLT
NELMELKKRY EKLSDRYNSL TPGYHKHKRQ EIKNKLIKLI ECMESKSDQI YLLYDVNVGK
DFS
