PPCS1_ARATH
ID PPCS1_ARATH Reviewed; 317 AA.
AC Q8GXR5; Q9LNB1;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Phosphopantothenate--cysteine ligase 1;
DE EC=6.3.2.51 {ECO:0000269|PubMed:12860978};
DE AltName: Full=AtCoaB {ECO:0000303|PubMed:12860978};
DE AltName: Full=Phosphopantothenoylcysteine synthetase 1;
DE Short=PPC synthetase 1;
GN Name=PPCS1; Synonyms=COAB, COAB1; OrderedLocusNames=At1g12350;
GN ORFNames=F5O11.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RC STRAIN=cv. Columbia;
RX PubMed=12860978; DOI=10.1074/jbc.m306321200;
RA Kupke T., Hernandez-Acosta P., Culianez-Macia F.A.;
RT "4'-phosphopantetheine and coenzyme A biosynthesis in plants.";
RL J. Biol. Chem. 278:38229-38237(2003).
CC -!- FUNCTION: Catalyzes the first step in the biosynthesis of coenzyme A
CC from vitamin B5/pantothenate, where cysteine is conjugated to 4'-
CC phosphopantothenate to form 4-phosphopantothenoylcysteine
CC (PubMed:12860978). The catalytic activity is not CTP- but ATP-dependent
CC (PubMed:12860978). {ECO:0000269|PubMed:12860978}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(R)-4'-phosphopantothenate + ATP + L-cysteine = AMP +
CC diphosphate + H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine;
CC Xref=Rhea:RHEA:25156, ChEBI:CHEBI:10986, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:35235,
CC ChEBI:CHEBI:59458, ChEBI:CHEBI:456215; EC=6.3.2.51;
CC Evidence={ECO:0000269|PubMed:12860978};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:25157;
CC Evidence={ECO:0000305|PubMed:12860978};
CC -!- PATHWAY: Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-
CC pantothenate: step 2/5.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the PPC synthetase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79631.1; Type=Erroneous gene model prediction; Note=The predicted gene has been split into 2 genes: At1g12340 and At1g12350.; Evidence={ECO:0000305};
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DR EMBL; AC025416; AAF79631.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28869.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM59452.1; -; Genomic_DNA.
DR EMBL; AK118086; BAC42714.1; -; mRNA.
DR RefSeq; NP_001321808.1; NM_001332031.1.
DR RefSeq; NP_563904.2; NM_101107.4.
DR AlphaFoldDB; Q8GXR5; -.
DR SMR; Q8GXR5; -.
DR STRING; 3702.AT1G12350.1; -.
DR PaxDb; Q8GXR5; -.
DR PRIDE; Q8GXR5; -.
DR ProteomicsDB; 249335; -.
DR EnsemblPlants; AT1G12350.1; AT1G12350.1; AT1G12350.
DR EnsemblPlants; AT1G12350.2; AT1G12350.2; AT1G12350.
DR GeneID; 837789; -.
DR Gramene; AT1G12350.1; AT1G12350.1; AT1G12350.
DR Gramene; AT1G12350.2; AT1G12350.2; AT1G12350.
DR KEGG; ath:AT1G12350; -.
DR Araport; AT1G12350; -.
DR TAIR; locus:2034690; AT1G12350.
DR eggNOG; KOG2728; Eukaryota.
DR HOGENOM; CLU_042326_0_1_1; -.
DR InParanoid; Q8GXR5; -.
DR OMA; WEVVFVT; -.
DR OrthoDB; 1292767at2759; -.
DR PhylomeDB; Q8GXR5; -.
DR BioCyc; ARA:AT1G12350-MON; -.
DR BioCyc; MetaCyc:AT1G12350-MON; -.
DR UniPathway; UPA00241; UER00353.
DR PRO; PR:Q8GXR5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8GXR5; baseline and differential.
DR Genevisible; Q8GXR5; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004632; F:phosphopantothenate--cysteine ligase activity; IDA:TAIR.
DR GO; GO:0015937; P:coenzyme A biosynthetic process; IDA:TAIR.
DR Gene3D; 3.40.50.10300; -; 1.
DR InterPro; IPR035929; CoaB-like_sf.
DR InterPro; IPR007085; DNA/pantothenate-metab_flavo_C.
DR Pfam; PF04127; DFP; 2.
DR SUPFAM; SSF102645; SSF102645; 1.
PE 1: Evidence at protein level;
KW Coenzyme A biosynthesis; Ligase; Reference proteome.
FT CHAIN 1..317
FT /note="Phosphopantothenate--cysteine ligase 1"
FT /id="PRO_0000398831"
SQ SEQUENCE 317 AA; 35369 MW; 8A39257F97D7A6A5 CRC64;
MSSISGLVED EISSFFESSP PLKNKEEIVA NLNQFIELNS SCQGGMRRIV CVTSGGTTVP
LEQRCVRYID NFSSGNRGAA STENFVKAGY AVIFLYRRGT CQPYCRYLPD DPFLECFEFP
DAKNIQVHGS HSGAVKMAVM DQQAAVAEGR LLKLPFSTIY EYLQMLRLIA TILKDVGPCS
MFYLAAAVSD FYVPWLSMTE HKIESGSGPL DIRLAQVPKM LSILRSNWAP KAFCISFKLE
TDSKILLEKA TKALQKYKVH AVVANELLTR KEEVVVVSSS GNVVVRRDSN KPESIVEDNL
IRLLVDRHST YIKESLT
