ID   PPCS2_ORYSJ             Reviewed;         323 AA.
AC   Q0J7N5; Q6ZK99;
DT   11-JUN-2014, integrated into UniProtKB/Swiss-Prot.
DT   11-JUN-2014, sequence version 2.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Phosphopantothenate--cysteine ligase 2;
DE            EC=6.3.2.5;
DE   AltName: Full=Phosphopantothenoylcysteine synthetase 2;
DE            Short=PPC synthetase 2;
GN   OrderedLocusNames=Os08g0176100, LOC_Os08g07880;
GN   ORFNames=OJ1134_B10.16, OsJ_36371;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Catalyzes the first step in the biosynthesis of coenzyme A
CC       from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate
CC       to form 4-phosphopantothenoylcysteine. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(R)-4'-phosphopantothenate + CTP + L-cysteine = CMP +
CC         diphosphate + H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine;
CC         Xref=Rhea:RHEA:19397, ChEBI:CHEBI:10986, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:35235, ChEBI:CHEBI:37563,
CC         ChEBI:CHEBI:59458, ChEBI:CHEBI:60377; EC=6.3.2.5;
CC   -!- PATHWAY: Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-
CC       pantothenate: step 2/5.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PPC synthetase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAF23030.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AP003882; BAD05232.1; -; Genomic_DNA.
DR   EMBL; AP008214; BAF23030.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014964; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CM000149; EEE53349.1; -; Genomic_DNA.
DR   EMBL; AK067868; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_015648433.1; XM_015792947.1.
DR   RefSeq; XP_015648435.1; XM_015792949.1.
DR   AlphaFoldDB; Q0J7N5; -.
DR   SMR; Q0J7N5; -.
DR   STRING; 4530.OS08T0176100-01; -.
DR   PaxDb; Q0J7N5; -.
DR   PRIDE; Q0J7N5; -.
DR   GeneID; 4344795; -.
DR   KEGG; osa:4344795; -.
DR   eggNOG; KOG2728; Eukaryota.
DR   HOGENOM; CLU_042326_0_1_1; -.
DR   InParanoid; Q0J7N5; -.
DR   OrthoDB; 1292767at2759; -.
DR   UniPathway; UPA00241; UER00353.
DR   Proteomes; UP000000763; Chromosome 8.
DR   Proteomes; UP000007752; Chromosome 12.
DR   Proteomes; UP000059680; Chromosome 8.
DR   Genevisible; Q0J7N5; OS.
DR   GO; GO:0004632; F:phosphopantothenate--cysteine ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015937; P:coenzyme A biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.10300; -; 1.
DR   InterPro; IPR035929; CoaB-like_sf.
DR   InterPro; IPR007085; DNA/pantothenate-metab_flavo_C.
DR   Pfam; PF04127; DFP; 2.
DR   SUPFAM; SSF102645; SSF102645; 1.
PE   2: Evidence at transcript level;
KW   Coenzyme A biosynthesis; Ligase; Reference proteome.
FT   CHAIN           1..323
FT                   /note="Phosphopantothenate--cysteine ligase 2"
FT                   /id="PRO_0000429410"
FT   CONFLICT        211
FT                   /note="P -> L (in Ref. 5; AK067868)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   323 AA;  35833 MW;  4AF2066C1AD2C402 CRC64;
     MAADPTTGGG EAESFFRAAP PLRDQDRVAG DLADFVARHS GSGGGGRLAG VVCVTSGGTT
     VPLEQRCVRY IDNFSSGQRG AASTEYFLKA GYAVIFIYRR GSKQPYCRFL PEDSFLDLFE
     LGEESDIQVP ESHAAVVKTA IRNYRKAIDE GLLLKLPFTT IFEYLQLLQM VGTAMNCLGR
     QGMFYLAAAV SDFYVPWESM AKHKIESASG PLNMQLNQVP KMLFILRKQW APSAFCVSFK
     LETDPDILLQ KAEAALRKYG MNVVVANELA NYKDVVVMVT SNGRTTVRRP SKEDDVEEQL
     IDLLVEMHSE HIMQLNQDVH KLT