ID   PPDA_CLOPE              Reviewed;         140 AA.
AC   P33555;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   31-JAN-2002, sequence version 2.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Prepilin peptidase-dependent protein A;
DE   Flags: Precursor;
GN   Name=ppdA; OrderedLocusNames=CPE2288;
OS   Clostridium perfringens (strain 13 / Type A).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=195102;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1347462; DOI=10.1016/0167-4781(92)90468-f;
RA   Rusanganwa E., Singh B., Gupta R.S.;
RT   "Cloning of HSP60 (GroEL) operon from Clostridium perfringens using a
RT   polymerase chain reaction based approach.";
RL   Biochim. Biophys. Acta 1130:90-94(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=13 / Type A;
RX   PubMed=11792842; DOI=10.1073/pnas.022493799;
RA   Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T.,
RA   Ogasawara N., Hattori M., Kuhara S., Hayashi H.;
RT   "Complete genome sequence of Clostridium perfringens, an anaerobic flesh-
RT   eater.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002).
RN   [3]
RP   IDENTIFICATION.
RX   PubMed=7934814; DOI=10.1111/j.1365-2958.1993.tb01949.x;
RA   Hobbs M., Mattick J.S.;
RT   "Common components in the assembly of type 4 fimbriae, DNA transfer
RT   systems, filamentous phage and protein-secretion apparatus: a general
RT   system for the formation of surface-associated protein complexes.";
RL   Mol. Microbiol. 10:233-243(1993).
CC   -!- FUNCTION: Not yet known.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC       protein {ECO:0000255}.
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DR   EMBL; X62914; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BA000016; BAB81994.1; -; Genomic_DNA.
DR   RefSeq; WP_011010863.1; NC_003366.1.
DR   AlphaFoldDB; P33555; -.
DR   SMR; P33555; -.
DR   STRING; 195102.gene:10491596; -.
DR   EnsemblBacteria; BAB81994; BAB81994; BAB81994.
DR   KEGG; cpe:CPE2288; -.
DR   HOGENOM; CLU_1831676_0_0_9; -.
DR   OMA; DKQNCAA; -.
DR   Proteomes; UP000000818; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR012902; N_methyl_site.
DR   InterPro; IPR045584; Pilin-like.
DR   Pfam; PF07963; N_methyl; 1.
DR   SUPFAM; SSF54523; SSF54523; 1.
DR   TIGRFAMs; TIGR02532; IV_pilin_GFxxxE; 1.
DR   PROSITE; PS00409; PROKAR_NTER_METHYL; 1.
PE   3: Inferred from homology;
KW   Membrane; Methylation; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   PROPEP          1..23
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000024272"
FT   CHAIN           24..140
FT                   /note="Prepilin peptidase-dependent protein A"
FT                   /id="PRO_0000024273"
FT   TRANSMEM        24..44
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         24
FT                   /note="N-methylphenylalanine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01070"
FT   CONFLICT        49
FT                   /note="Y -> S (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        83
FT                   /note="V -> I (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        123
FT                   /note="D -> E (in Ref. 1)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   140 AA;  14931 MW;  17063CA23C158981 CRC64;
     MLLLKASAIC GKGNEGKRNK KGGFTLIELT VVLAIMAIIL MVIAPNFSYV KDSAKAKVDK
     QNCAAIERSV EMLLAEDAIS SSVTNIKITS SNGNVQISGI SDDTGKSKLQ DLLEDLDKPQ
     SGDSYNVDIE NGRKVTVSIV