ID   PPDA_ECOLI              Reviewed;         156 AA.
AC   P33554; Q2MA11; Q59379;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   29-AUG-2003, sequence version 2.
DT   03-AUG-2022, entry version 133.
DE   RecName: Full=Prepilin peptidase-dependent protein A;
DE   Flags: Precursor;
GN   Name=ppdA; OrderedLocusNames=b2826, JW2794;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3520484; DOI=10.1093/nar/14.11.4437;
RA   Finch P.W., Wilson R.E., Brown K., Hickson I.D., Tomkinson A.E.,
RA   Emmerson P.T.;
RT   "Complete nucleotide sequence of the Escherichia coli recC gene and of the
RT   thyA-recC intergenic region.";
RL   Nucleic Acids Res. 14:4437-4451(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   IDENTIFICATION.
RX   PubMed=7934814; DOI=10.1111/j.1365-2958.1993.tb01949.x;
RA   Hobbs M., Mattick J.S.;
RT   "Common components in the assembly of type 4 fimbriae, DNA transfer
RT   systems, filamentous phage and protein-secretion apparatus: a general
RT   system for the formation of surface-associated protein complexes.";
RL   Mol. Microbiol. 10:233-243(1993).
CC   -!- FUNCTION: Not yet known.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC       protein {ECO:0000255}.
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DR   EMBL; X03966; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; U29581; AAB40473.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75865.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76895.1; -; Genomic_DNA.
DR   PIR; C65065; C65065.
DR   RefSeq; NP_417303.1; NC_000913.3.
DR   RefSeq; WP_000857051.1; NZ_STEB01000034.1.
DR   AlphaFoldDB; P33554; -.
DR   SMR; P33554; -.
DR   BioGRID; 4262027; 210.
DR   STRING; 511145.b2826; -.
DR   PaxDb; P33554; -.
DR   PRIDE; P33554; -.
DR   EnsemblBacteria; AAC75865; AAC75865; b2826.
DR   EnsemblBacteria; BAE76895; BAE76895; BAE76895.
DR   GeneID; 66673307; -.
DR   GeneID; 945393; -.
DR   KEGG; ecj:JW2794; -.
DR   KEGG; eco:b2826; -.
DR   PATRIC; fig|511145.12.peg.2924; -.
DR   EchoBASE; EB2006; -.
DR   eggNOG; COG2165; Bacteria.
DR   HOGENOM; CLU_118590_1_0_6; -.
DR   OMA; LRNTAWP; -.
DR   PhylomeDB; P33554; -.
DR   BioCyc; EcoCyc:EG12081-MON; -.
DR   PRO; PR:P33554; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR012902; N_methyl_site.
DR   InterPro; IPR045584; Pilin-like.
DR   Pfam; PF07963; N_methyl; 1.
DR   SUPFAM; SSF54523; SSF54523; 1.
DR   TIGRFAMs; TIGR02532; IV_pilin_GFxxxE; 1.
DR   PROSITE; PS00409; PROKAR_NTER_METHYL; 1.
PE   3: Inferred from homology;
KW   Membrane; Methylation; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   PROPEP          1..6
FT                   /note="Leader sequence"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01070"
FT                   /id="PRO_0000024274"
FT   CHAIN           7..156
FT                   /note="Prepilin peptidase-dependent protein A"
FT                   /id="PRO_0000024275"
FT   TRANSMEM        7..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         7
FT                   /note="N-methyltyrosine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01070"
FT   CONFLICT        5
FT                   /note="R -> A (in Ref. 1)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   156 AA;  17865 MW;  B087405B39F9A432 CRC64;
     MKTQRGYTLI ETLVAMLILV MLSASGLYGW QYWQQSQRLW QTASQARDYL LYLREDANWH
     NRDHSISVIR EGTLWCLVSS AAGANTCHGS SPLVFVPRWP EVEMSDLTPS LAFFGLRNTA
     WAGHIRFKNS TGEWWLVVSP WGRLRLCQQG ETEGCL