ID   PPE15_MYCTU             Reviewed;         391 AA.
AC   P9WI31; L0T5J3; Q79FT5; Q7D8Y7;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 32.
DE   RecName: Full=PPE family protein PPE15 {ECO:0000305};
DE   AltName: Full=Mycobacterial perilipin-1 {ECO:0000303|PubMed:27325376};
DE            Short=MPER1 {ECO:0000303|PubMed:27325376};
GN   Name=PPE15; Synonyms=mper1 {ECO:0000303|PubMed:27325376};
GN   OrderedLocusNames=Rv1039c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=27325376; DOI=10.1111/mmi.13422;
RA   Daniel J., Kapoor N., Sirakova T., Sinha R., Kolattukudy P.;
RT   "The perilipin-like PPE15 protein in Mycobacterium tuberculosis is required
RT   for triacylglycerol accumulation under dormancy-inducing conditions.";
RL   Mol. Microbiol. 101:784-794(2016).
CC   -!- FUNCTION: May play a critical role in the homeostasis of
CC       triacylglycerol-containing lipid droplets in M.tuberculosis and
CC       influence the entry of the pathogen into a dormant state.
CC       {ECO:0000269|PubMed:27325376}.
CC   -!- INDUCTION: Up-regulated in tuberculosis dormancy.
CC       {ECO:0000305|PubMed:27325376}.
CC   -!- DISRUPTION PHENOTYPE: Disruption mutant shows a significant decrease in
CC       the biosynthesis and accumulation of lipid droplets containing
CC       triacylglycerol and in its tolerance to rifampicin.
CC       {ECO:0000269|PubMed:27325376}.
CC   -!- SIMILARITY: Belongs to the mycobacterial PPE family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP43790.1; -; Genomic_DNA.
DR   PIR; B70625; B70625.
DR   RefSeq; WP_003405368.1; NZ_NVQJ01000098.1.
DR   RefSeq; YP_177778.1; NC_000962.3.
DR   PDB; 5XFS; X-ray; 2.90 A; B=1-194.
DR   PDBsum; 5XFS; -.
DR   AlphaFoldDB; P9WI31; -.
DR   SMR; P9WI31; -.
DR   STRING; 83332.Rv1039c; -.
DR   PaxDb; P9WI31; -.
DR   DNASU; 888477; -.
DR   GeneID; 888477; -.
DR   KEGG; mtu:Rv1039c; -.
DR   TubercuList; Rv1039c; -.
DR   eggNOG; COG5651; Bacteria.
DR   OMA; MDIRAGL; -.
DR   PhylomeDB; P9WI31; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0052572; P:response to host immune response; IBA:GO_Central.
DR   Gene3D; 1.20.1260.20; -; 1.
DR   InterPro; IPR022171; PPE_C.
DR   InterPro; IPR000030; PPE_family.
DR   InterPro; IPR038332; PPE_sf.
DR   Pfam; PF00823; PPE; 1.
DR   Pfam; PF12484; PPE-SVP; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Reference proteome.
FT   CHAIN           1..391
FT                   /note="PPE family protein PPE15"
FT                   /id="PRO_0000378477"
FT   HELIX           8..13
FT                   /evidence="ECO:0007829|PDB:5XFS"
FT   STRAND          16..18
FT                   /evidence="ECO:0007829|PDB:5XFS"
FT   HELIX           22..55
FT                   /evidence="ECO:0007829|PDB:5XFS"
FT   TURN            56..58
FT                   /evidence="ECO:0007829|PDB:5XFS"
FT   HELIX           59..61
FT                   /evidence="ECO:0007829|PDB:5XFS"
FT   HELIX           62..103
FT                   /evidence="ECO:0007829|PDB:5XFS"
FT   HELIX           107..122
FT                   /evidence="ECO:0007829|PDB:5XFS"
FT   STRAND          125..127
FT                   /evidence="ECO:0007829|PDB:5XFS"
FT   HELIX           130..161
FT                   /evidence="ECO:0007829|PDB:5XFS"
SQ   SEQUENCE   391 AA;  38081 MW;  AFDF3EA4FB195C4F CRC64;
     MDFGALPPEI NSARMYAGAG AGPMMAAGAA WNGLAAELGT TAASYESVIT RLTTESWMGP
     ASMAMVAAAQ PYLAWLTYTA EAAAHAGSQA MASAAAYEAA YAMTVPPEVV AANRALLAAL
     VATNVLGINT PAIMATEALY AEMWAQDALA MYGYAAASGA AGMLQPLSPP SQTTNPGGLA
     AQSAAVGSAA ATAAVNQVSV ADLISSLPNA VSGLASPVTS VLDSTGLSGI IADIDALLAT
     PFVANIINSA VNTAAWYVNA AIPTAIFLAN ALNSGAPVAI AEGAIEAAEG AASAAAAGLA
     DSVTPAGLGA SLGEATLVGR LSVPAAWSTA APATTAGATA LEGSGWTVAA EEAGPVTGMM
     PGMASAAKGT GAYAGPRYGF KPTVMPKQVV V