ID   PPE20_MYCTU             Reviewed;         539 AA.
AC   P9WI23; L0T6I0; Q79FP7; Q7D8H6;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 32.
DE   RecName: Full=Uncharacterized PPE family protein PPE20 {ECO:0000305};
GN   Name=PPE20; OrderedLocusNames=Rv1387;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [3]
RP   SUBCELLULAR LOCATION.
RX   PubMed=26729876; DOI=10.1073/pnas.1523321113;
RA   Tufariello J.M., Chapman J.R., Kerantzas C.A., Wong K.W., Vilcheze C.,
RA   Jones C.M., Cole L.E., Tinaztepe E., Thompson V., Fenyoe D., Niederweis M.,
RA   Ueberheide B., Philips J.A., Jacobs W.R. Jr.;
RT   "Separable roles for Mycobacterium tuberculosis ESX-3 effectors in iron
RT   acquisition and virulence.";
RL   Proc. Natl. Acad. Sci. U.S.A. 113:E348-E357(2016).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:26729876}.
CC       Note=Secreted via the ESX-3 / type VII secretion system (T7SS)
CC       (PubMed:26729876). Secretion is dependent on EsxG and EsxH
CC       (PubMed:26729876). {ECO:0000269|PubMed:26729876}.
CC   -!- SIMILARITY: Belongs to the mycobacterial PPE family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP44146.1; -; Genomic_DNA.
DR   PIR; A70899; A70899.
DR   RefSeq; WP_003407230.1; NZ_NVQJ01000050.1.
DR   RefSeq; YP_177806.1; NC_000962.3.
DR   AlphaFoldDB; P9WI23; -.
DR   SMR; P9WI23; -.
DR   STRING; 83332.Rv1387; -.
DR   PaxDb; P9WI23; -.
DR   GeneID; 886784; -.
DR   KEGG; mtu:Rv1387; -.
DR   TubercuList; Rv1387; -.
DR   eggNOG; COG5651; Bacteria.
DR   PhylomeDB; P9WI23; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0052572; P:response to host immune response; IBA:GO_Central.
DR   Gene3D; 1.20.1260.20; -; 1.
DR   InterPro; IPR043641; PPE-PPW_C.
DR   InterPro; IPR000030; PPE_family.
DR   InterPro; IPR038332; PPE_sf.
DR   Pfam; PF00823; PPE; 1.
DR   Pfam; PF18878; PPE-PPW; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Secreted.
FT   CHAIN           1..539
FT                   /note="Uncharacterized PPE family protein PPE20"
FT                   /id="PRO_0000379110"
FT   REGION          179..203
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          433..459
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        189..203
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   539 AA;  55404 MW;  7FE63A9BAC6FBEB5 CRC64;
     MTEPWIAFPP EVHSAMLNYG AGVGPMLISA TQNGELSAQY AEAASEVEEL LGVVASEGWQ
     GQAAEAFVAA YMPFLAWLIQ ASADCVEMAA QQHVVIEAYT AAVELMPTQV ELAANQIKLA
     VLVATNFFGI NTIPIAINEA EYVEMWVRAA TTMATYSTVS RSALSAMPHT SPPPLILKSD
     ELLPDTGEDS DEDGHNHGGH SHGGHARMID NFFAEILRGV SAGRIVWDPV NGTLNGLDYD
     DYVYPGHAIW WLARGLEFFQ DGEQFGELLF TNPTGAFQFL LYVVVVDLPT HIAQIATWLG
     QYPQLLSAAL TGVIAHLGAI TGLAGLSGLS AIPSAAIPAV VPELTPVAAA PPMLAVAGVG
     PAVAAPGMLP ASAPAPAAAA GATAAGPTPP ATGFGGFPPY LVGGGGPGIG FGSGQSAHAK
     AAASDSAAAE SAAQASARAQ ARAARRGRSA AKARGHRDEF VTMDMGFDAA APAPEHQPGA
     RASDCGAGPI GFAGTVRKEA VVKAAGLTTL AGDDFGGGPT MPMMPGTWTH DQGVFDEHR