ID   PPE46_MYCTU             Reviewed;         434 AA.
AC   P9WHY9; L0TCW9; O53265; P31500;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 33.
DE   RecName: Full=Uncharacterized PPE family protein PPE46;
GN   Name=PPE46; OrderedLocusNames=Rv3018c; ORFNames=MTV012.32c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 160-374.
RC   STRAIN=Isolate 50410;
RA   Patki A.H., Dale J.W.;
RL   Submitted (APR-1991) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   BIOTECHNOLOGY.
RC   STRAIN=H37Rv;
RX   PubMed=18719176; DOI=10.1099/jmm.0.47565-0;
RA   Chaitra M.G., Shaila M.S., Nayak R.;
RT   "Characterization of T-cell immunogenicity of two PE/PPE proteins of
RT   Mycobacterium tuberculosis.";
RL   J. Med. Microbiol. 57:1079-1086(2008).
RN   [4]
RP   IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
RX   PubMed=19099550; DOI=10.1186/1752-0509-2-109;
RA   Raman K., Yeturu K., Chandra N.;
RT   "targetTB: a target identification pipeline for Mycobacterium tuberculosis
RT   through an interactome, reactome and genome-scale structural analysis.";
RL   BMC Syst. Biol. 2:109-109(2008).
CC   -!- BIOTECHNOLOGY: Exhibits vaccine potential. Immunogenicity is
CC       demonstrated in BALB/c mice. Immunization with this protein induces a
CC       significant number of CD8+ T cells and a strong Th1-type response, with
CC       high gamma interferon (IFN-gamma) and low interleukin-4 responses, and
CC       activation of the bactericidal activity of macrophages. Identified T-
CC       cell epitopes may contribute to immunity against tuberculosis if
CC       included in a vaccine. {ECO:0000269|PubMed:18719176}.
CC   -!- MISCELLANEOUS: Was identified as a high-confidence drug target.
CC       {ECO:0000269|PubMed:19099550}.
CC   -!- SIMILARITY: Belongs to the mycobacterial PPE family. {ECO:0000305}.
CC   -!- CAUTION: Was originally thought to be a dihydrofolate reductase.
CC       {ECO:0000305|Ref.2}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA41961.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; X59271; CAA41961.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; AL123456; CCP45824.1; -; Genomic_DNA.
DR   PIR; E70857; E70857.
DR   RefSeq; WP_003910691.1; NC_018143.2.
DR   RefSeq; YP_177918.1; NC_000962.3.
DR   AlphaFoldDB; P9WHY9; -.
DR   SMR; P9WHY9; -.
DR   STRING; 83332.Rv3018c; -.
DR   PaxDb; P9WHY9; -.
DR   GeneID; 888940; -.
DR   KEGG; mtu:Rv3018c; -.
DR   TubercuList; Rv3018c; -.
DR   eggNOG; COG5651; Bacteria.
DR   OMA; HWAGESL; -.
DR   PhylomeDB; P9WHY9; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0051701; P:biological process involved in interaction with host; IMP:MTBBASE.
DR   GO; GO:0052572; P:response to host immune response; IBA:GO_Central.
DR   Gene3D; 1.20.1260.20; -; 1.
DR   InterPro; IPR043641; PPE-PPW_C.
DR   InterPro; IPR000030; PPE_family.
DR   InterPro; IPR038332; PPE_sf.
DR   Pfam; PF00823; PPE; 1.
DR   Pfam; PF18878; PPE-PPW; 1.
PE   1: Evidence at protein level;
KW   Reference proteome.
FT   CHAIN           1..434
FT                   /note="Uncharacterized PPE family protein PPE46"
FT                   /id="PRO_0000217851"
SQ   SEQUENCE   434 AA;  43029 MW;  41D673C4BD389DD6 CRC64;
     MTAPVWLASP PEVHSALLSA GPGPGSLQAA AAGWSALSAE YAAVAQELSV VVAAVGAGVW
     QGPSAELFVA AYVPYVAWLV QASADSAAAA GEHEAAAAGY VCALAEMPTL PELAANHLTH
     AVLVATNFFG INTIPIALNE ADYVRMWVQA ATVMSAYEAV VGAALVATPH TGPAPVIVKP
     GANEASNAVA AATITPFPWH EIVQFLEETF AAYDQYLSAL LSELPAVAWV WFQLFVDILG
     FNIIGFIITL ASNAQLLTEF AINASYVAVG LLYAIAGVID IVVEWVIGNL FGVVPLLGGP
     LLGALAAAVV PGVAGLAGVA GLAALPAVGA AAGAPAALVG SVAPVSGGVV SPQARLVSAV
     EPAPASTSVS VLASDRGAGA LGFVGTAGKE SVGQPAGLTV LADEFGDGAP VPMLPGSWGP
     DLVGVAGDGG LVSV