ID   PPE4_PHYPN              Reviewed;         134 AA.
AC   W2RE78;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   19-MAR-2014, sequence version 1.
DT   25-MAY-2022, entry version 22.
DE   RecName: Full=RxLR effector protein 4 {ECO:0000303|PubMed:30320960};
DE   Flags: Precursor;
GN   Name=E4 {ECO:0000303|PubMed:30320960}; ORFNames=PPTG_00121;
OS   Phytophthora parasitica (strain INRA-310).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=761204;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=INRA-310;
RG   The Broad Institute Genome Sequencing Platform;
RA   Russ C., Tyler B., Panabieres F., Shan W., Tripathy S., Grunwald N.,
RA   Machado M., Young S.K., Zeng Q., Gargeya S., Fitzgerald M., Haas B.,
RA   Abouelleil A., Alvarado L., Arachchi H.M., Berlin A., Chapman S.B.,
RA   Gearin G., Goldberg J., Griggs A., Gujja S., Hansen M., Heiman D.,
RA   Howarth C., Larimer J., Lui A., MacDonald P.J.P., McCowen C.,
RA   Montmayeur A., Murphy C., Neiman D., Pearson M., Priest M., Roberts A.,
RA   Saif S., Shea T., Sisk P., Stolte C., Sykes S., Wortman J., Nusbaum C.,
RA   Birren B.;
RL   Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=INRA-310;
RG   The Broad Institute Genomics Platform;
RA   Russ C., Tyler B., Panabieres F., Shan W., Tripathy S., Grunwald N.,
RA   Machado M., Johnson C.S., Arredondo F., Hong C., Coffey M., Young S.K.,
RA   Zeng Q., Gargeya S., Fitzgerald M., Abouelleil A., Alvarado L.,
RA   Chapman S.B., Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M.,
RA   Howarth C., Imamovic A., Ireland A., Larimer J., McCowan C., Murphy C.,
RA   Pearson M., Poon T.W., Priest M., Roberts A., Saif S., Shea T., Sykes S.,
RA   Wortman J., Nusbaum C., Birren B.;
RT   "The Genome Sequence of Phytophthora parasitica INRA-310.";
RL   Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, INDUCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX   PubMed=30320960; DOI=10.1111/mpp.12760;
RA   Huang G., Liu Z., Gu B., Zhao H., Jia J., Fan G., Meng Y., Du Y., Shan W.;
RT   "An RXLR effector secreted by Phytophthora parasitica is a virulence factor
RT   and triggers cell death in various plants.";
RL   Mol. Plant Pathol. 20:356-371(2019).
CC   -!- FUNCTION: Effector that enhances plant susceptibility to P.parasitica
CC       in Nicotiana benthamiana and Arabidopsis thaliana. Triggers non-
CC       specific cell death in a variety of plants, including tobacco, tomato,
CC       potato and A.thaliana. E4-induced cell death is dependent on HSP90, NPK
CC       and SGT1, suggesting that PpE4 is recognized by the plant immune
CC       system. {ECO:0000269|PubMed:30320960}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30320960}.
CC       Note=Accumulates around haustoria during plant infection.
CC       {ECO:0000269|PubMed:30320960}.
CC   -!- INDUCTION: Expression is highly induced during the early stages of
CC       infection. {ECO:0000269|PubMed:30320960}.
CC   -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC       cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC       phosphate. {ECO:0000305|PubMed:30320960}.
CC   -!- DISRUPTION PHENOTYPE: Results in significantly reduced virulence on
CC       Nicotiana benthamiana. {ECO:0000269|PubMed:30320960}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; KI669561; ETN23541.1; -; Genomic_DNA.
DR   RefSeq; XP_008889734.1; XM_008891486.1.
DR   AlphaFoldDB; W2RE78; -.
DR   EnsemblProtists; ETN23541; ETN23541; PPTG_00121.
DR   GeneID; 20170517; -.
DR   VEuPathDB; FungiDB:PPTG_00121; -.
DR   OMA; GRITQKN; -.
DR   Proteomes; UP000018817; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR031825; RXLR.
DR   Pfam; PF16810; RXLR; 1.
PE   2: Evidence at transcript level;
KW   Reference proteome; Secreted; Signal; Virulence.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..134
FT                   /note="RxLR effector protein 4"
FT                   /id="PRO_5004824638"
FT   REGION          43..65
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           52..71
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:30320960"
FT   COMPBIAS        48..65
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   134 AA;  14774 MW;  6A6A3D25D8792279 CRC64;
     MRSLFYIAVA VAVFARSSAV AAFTNADDSQ LLSKTTPDFA TDAMASSDSR KRFLRATDPE
     DGDLQADDEE RTKFKSLADI IKHLDDQDMK HVAGILANMD DIHHKNVLAK ALESGRITQK
     NYDDAIAALQ RTSK