ID   PPE54_MYCTU             Reviewed;        2523 AA.
AC   Q6MWY2; L0TCH9;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Uncharacterized PPE family protein PPE54;
GN   Name=PPE54; OrderedLocusNames=Rv3343c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
RX   PubMed=19099550; DOI=10.1186/1752-0509-2-109;
RA   Raman K., Yeturu K., Chandra N.;
RT   "targetTB: a target identification pipeline for Mycobacterium tuberculosis
RT   through an interactome, reactome and genome-scale structural analysis.";
RL   BMC Syst. Biol. 2:109-109(2008).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=Beijing GC1237;
RX   PubMed=20844580; DOI=10.1371/journal.ppat.1001100;
RA   Brodin P., Poquet Y., Levillain F., Peguillet I., Larrouy-Maumus G.,
RA   Gilleron M., Ewann F., Christophe T., Fenistein D., Jang J., Jang M.S.,
RA   Park S.J., Rauzier J., Carralot J.P., Shrimpton R., Genovesio A.,
RA   Gonzalo-Asensio J.A., Puzo G., Martin C., Brosch R., Stewart G.R.,
RA   Gicquel B., Neyrolles O.;
RT   "High content phenotypic cell-based visual screen identifies Mycobacterium
RT   tuberculosis acyltrehalose-containing glycolipids involved in phagosome
RT   remodeling.";
RL   PLoS Pathog. 6:E1001100-E1001100(2010).
CC   -!- FUNCTION: Probably plays a role in host phagosome maturation arrest
CC       (PubMed:20844580). {ECO:0000305|PubMed:20844580}.
CC   -!- DISRUPTION PHENOTYPE: Grows normally in liquid culture, traffics into
CC       host (human and mouse) acidified compartments early after phagocytosis,
CC       suggesting it no longer arrests phagosome maturation as well as wild-
CC       type, impaired growth in mouse macrophages (PubMed:20844580).
CC       {ECO:0000269|PubMed:20844580}.
CC   -!- MISCELLANEOUS: Was identified as a high-confidence drug target.
CC   -!- SIMILARITY: Belongs to the mycobacterial PPE family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP46164.1; -; Genomic_DNA.
DR   PIR; F70846; F70846.
DR   RefSeq; WP_010886163.1; NC_018143.2.
DR   RefSeq; YP_177960.1; NC_000962.3.
DR   AlphaFoldDB; Q6MWY2; -.
DR   STRING; 83332.Rv3343c; -.
DR   PaxDb; Q6MWY2; -.
DR   GeneID; 888033; -.
DR   KEGG; mtu:Rv3343c; -.
DR   TubercuList; Rv3343c; -.
DR   eggNOG; COG5651; Bacteria.
DR   OMA; VYIDTHP; -.
DR   PhylomeDB; Q6MWY2; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0052572; P:response to host immune response; IBA:GO_Central.
DR   GO; GO:0052170; P:suppression by symbiont of host innate immune response; IDA:MTBBASE.
DR   Gene3D; 1.20.1260.20; -; 1.
DR   InterPro; IPR002989; Mycobac_pentapep.
DR   InterPro; IPR000030; PPE_family.
DR   InterPro; IPR038332; PPE_sf.
DR   Pfam; PF01469; Pentapeptide_2; 16.
DR   Pfam; PF00823; PPE; 1.
PE   3: Inferred from homology;
KW   Reference proteome.
FT   CHAIN           1..2523
FT                   /note="Uncharacterized PPE family protein PPE54"
FT                   /id="PRO_0000378486"
SQ   SEQUENCE   2523 AA;  250346 MW;  CD0DADA4040669B3 CRC64;
     MSFVVMPPEI NSLLIYTGAG PGPLLAAAAA WDELAAELGS AAAAFGSVTS GLVGGIWQGP
     SSVAMAAAAA PYAGWLSAAA ASAESAAGQA RAVVGVFEAA LAETVDPFVI AANRSRLVSL
     ALSNLFGQNT PAIAAAEFDY ELMWAQDVAA MLGYHTGASA AAEALAPFGS PLASLAAAAE
     PAKSLAVNLG LANVGLFNAG SGNVGSYNVG AGNVGSYNVG GGNIGGNNVG LGNVGWGNFG
     LGNSGLTPGL MGLGNIGFGN AGSYNFGLAN MGVGNIGFAN TGSGNFGIGL TGDNLTGFGG
     FNTGSGNVGL FNSGTGNVGF FNSGTGNWGV FNSGSYNTGI GNSGIASTGL FNAGGFNTGV
     VNAGSYNTGS FNAGEANTGG FNPGSVNTGW LNTGDINTGV ANSGDVNTGA FISGNYSNGV
     LWRGDYQGLL GFSSGANVLP VIPLSLDING GVGAITIEPI HILPDIPINI NETLYLGPLV
     VPPINVPAIS LGVGIPNISI GPIKINPITL WPAQNFNQTI TLAWPVSSIT IPQIQQVALS
     PSPIPTTLIG PIHINTGFSI PVTFSYSTPA LTLFPVGLSI PTGGPLTLTL GVTAGTEAFT
     IPGFSIPEQP LPLAINVIGH INALSTPAIT IDNIPLNLHA IGGVGPVDIV GGNVPASPGF
     GNSTTAPSSG FFNTGAGGVS GFGNVGAHTS GWFNQSTQAM QVLPGTVSGY FNSGTLMSGI
     GNVGTQLSGM LSGGALGGNN FGLGNIGFDN VGFGNAGSSN FGLANMGIGN IGLANTGNGN
     IGIGLSGDNL TGFGGFNSGS ENVGLFNSGT GNVGFFNSGT GNLGVFNSGS HNTGFFLTGN
     NINVLAPFTP GTLFTISEIP IDLQVIGGIG PIHVQPIDIP AFDIQITGGF IGIREFTLPE
     ITIPAIPIHV TGTVGLEGFH VNPAFVLFGQ TAMAEITADP VVLPDPFITI DHYGPPLGPP
     GAKFPSGSFY LSISDLQING PIIGSYGGPG TIPGPFGATF NLSTSSLALF PAGLTVPDQT
     PVTVNLTGGL DSITLFPGGL AFPENPVVSL TNFSVGTGGF TVFPQGFTVD RIPVDLHTTL
     SIGPFPFRWD YIPPTPANGP IPAVPGGFGL TSGLFPFHFT LNGGIGPISI PTTTVVDALN
     PLLTVTGNLE VGPFTVPDIP IPAINFGLDG NVNVSFNAPA TTLLSGLGIT GSIDISGIQI
     TNIQTQPAQL FMSVGQTLFL FDFRDGIELN PIVIPGSSIP ITMAGLSIPL PTVSESIPLN
     FSFGSPASTV KSMILHEILP IDVSINLEDA VFIPATVLPA IPLNVDVTIP VGPINIPIIT
     EPGSGNSTTT TSDPFSGLAV PGLGVGLLGL FDGSIANNLI SGFNSAVGIV GPNVGLSNLG
     GGNVGLGNVG DFNLGAGNVG GFNVGGGNIG GNNVGLGNVG FGNVGLANSG LTPGLMGLGN
     IGFGNAGSYN FGLANMGVGN IGFANTGSGN FGIGLTGDNL TGFGGFNTGS GNVGLFNSGT
     GNVGFFNSGT GNWGVFNSGS YNTGIGNSGI ASTGLFNAGG FNTGVVNAGS YNTGSFNAGQ
     ANTGGFNPGS VNTGWLNTGD INTGVANSGD VNTGAFISGN YSNGAFWRGD YQGLLGFSYR
     PAVLPQTPFL DLTLTGGLGS VVIPAIDIPA IRPEFSANVA IDSFTVPSIP IPQIDLAATT
     VSVGLGPITV PHLDIPRVPV TLNYLFGSQP GGPLKIGPIT GLFNTPIGLT PLALSQIVIG
     ASSSQGTITA FLANLPFSTP VVTIDEIPLL ASITGHSEPV DIFPGGLTIP AMNPLSINLS
     GGTGAVTIPA ITIGEIPFDL VAHSTLGPVH ILIDLPAVPG FGNTTGAPSS GFFNSGAGGV
     SGFGNVGAMV SGGWNQAPSA LLGGGSGVFN AGTLHSGVLN FGSGMSGLFN TSVLGLGAPA
     LVSGLGSVGQ QLSGLLASGT ALHQGLVLNF GLADVGLGNV GLGNVGDFNL GAGNVGGFNV
     GGGNIGGNNV GLGNVGWGNF GLGNSGLTPG LMGLGNIGFG NAGSYNFGLA NMGVGNIGFA
     NTGSGNFGIG LTGDNLTGFG GFNTGSGNVG LFNSGTGNVG FFNSGTGNWG VFNSGSYNTG
     IGNSGIASTG LFNAGGFNTG VVNAGSYNTG SFNAGQANTG GFNPGSVNTG WLNTGDINTG
     VANSGDVNTG AFISGNYSNG AFWRGDYQGL LGFSYTSTII PEFTVANIHA SGGAGPIIVP
     SIQFPAIPLD LSATGHIGGF TIPPVSISPI TVRIDPVFDL GPITVQDITI PALGLDPATG
     VTVGPIFSSG SIIDPFSLTL LGFINVNVPA IQTAPSEILP FTVLLSSLGV THLTPEITIP
     GFHIPVDPIH VELPLSVTIG PFVSPEITIP QLPLGLALSG ATPAFAFPLE ITIDRIPVVL
     DVNALLGPIN AGLVIPPVPG FGNTTAVPSS GFFNIGGGGG LSGFHNLGAG MSGVLNAISD
     PLLGSASGFA NFGTQLSGIL NRGADISGVY NTGALGLITS ALVSGFGNVG QQLAGLIYTG
     TGP