PPE57_MYCTU
ID PPE57_MYCTU Reviewed; 176 AA.
AC Q50703; L0TE36;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=PPE family protein PPE57 {ECO:0000305};
GN Name=PPE57 {ECO:0000303|PubMed:19467342, ECO:0000312|EMBL:CCP46247.1};
GN OrderedLocusNames=Rv3425 {ECO:0000312|EMBL:CCP46247.1};
GN ORFNames=MTCY78.04c;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP FUNCTION, INDUCTION, AND BIOTECHNOLOGY.
RC STRAIN=H37Rv;
RX PubMed=17328725; DOI=10.1111/j.1469-0691.2006.01561.x;
RA Zhang H., Wang J., Lei J., Zhang M., Yang Y., Chen Y., Wang H.;
RT "PPE protein (Rv3425) from DNA segment RD11 of Mycobacterium tuberculosis:
RT a potential B-cell antigen used for serological diagnosis to distinguish
RT vaccinated controls from tuberculosis patients.";
RL Clin. Microbiol. Infect. 13:139-145(2007).
RN [3]
RP FUNCTION, AND BIOTECHNOLOGY.
RX PubMed=18426397; DOI=10.1111/j.1348-0421.2008.00029.x;
RA Wang J., Qie Y., Zhang H., Zhu B., Xu Y., Liu W., Chen J., Wang H.;
RT "PPE protein (Rv3425) from DNA segment RD11 of Mycobacterium tuberculosis:
RT a novel immunodominant antigen of Mycobacterium tuberculosis induces
RT humoral and cellular immune responses in mice.";
RL Microbiol. Immunol. 52:224-230(2008).
RN [4]
RP FUNCTION, AND BIOTECHNOLOGY.
RX PubMed=19467342; DOI=10.1016/j.micinf.2009.05.008;
RA Chen J., Su X., Zhang Y., Wang S., Shao L., Wu J., Wang F., Zhang S.,
RA Wang J., Weng X., Wang H., Zhang W.;
RT "Novel recombinant RD2- and RD11-encoded Mycobacterium tuberculosis
RT antigens are potential candidates for diagnosis of tuberculosis infections
RT in BCG-vaccinated individuals.";
RL Microbes Infect. 11:876-885(2009).
RN [5]
RP FUNCTION, AND BIOTECHNOLOGY.
RX PubMed=23136116; DOI=10.1128/cvi.00481-12;
RA Wang S., Chen J., Zhang Y., Diao N., Zhang S., Wu J., Lu C., Wang F.,
RA Gao Y., Shao L., Jin J., Weng X., Zhang W.;
RT "Mycobacterium tuberculosis region of difference (RD) 2 antigen Rv1985c and
RT RD11 antigen Rv3425 have the promising potential to distinguish patients
RT with active tuberculosis from M. bovis BCG-vaccinated individuals.";
RL Clin. Vaccine Immunol. 20:69-76(2013).
RN [6]
RP FUNCTION, INTERACTION WITH TLR2, SUBCELLULAR LOCATION, AND BIOTECHNOLOGY.
RC STRAIN=H37Rv;
RX PubMed=25586105; DOI=10.1007/s00109-014-1243-1;
RA Xu Y., Yang E., Huang Q., Ni W., Kong C., Liu G., Li G., Su H., Wang H.;
RT "PPE57 induces activation of macrophages and drives Th1-type immune
RT responses through TLR2.";
RL J. Mol. Med. 93:645-662(2015).
CC -!- FUNCTION: Plays a key role in regulating innate and adaptive immune
CC responses through human Toll-like receptor 2 (TLR2). Interacts with
CC TLR2, leading to the subsequent activation of the mitogen-activated
CC protein kinase (MAPK) and nuclear factor kappa B (NF-kappa-B) signaling
CC pathways. Induces macrophage activation by augmenting the expression of
CC several cell surface molecules (CD40, CD80, CD86 and MHC class II) and
CC pro-inflammatory cytokines (TNF-alpha, IL-6 and IL-12p40) within
CC macrophages. Also participates in adaptive immunity by directing Th1-
CC polarised immune responses (PubMed:25586105). Stimulates specific
CC humoral and cellular immune responses in tuberculosis (TB) patients
CC (PubMed:17328725, PubMed:19467342, PubMed:23136116). Induces a strong
CC IgG(1) antibody response and an increased Th1/Th2 type immune response
CC in mice (PubMed:18426397). {ECO:0000269|PubMed:17328725,
CC ECO:0000269|PubMed:18426397, ECO:0000269|PubMed:19467342,
CC ECO:0000269|PubMed:23136116, ECO:0000269|PubMed:25586105}.
CC -!- SUBUNIT: Interacts with human TLR2. {ECO:0000269|PubMed:25586105}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC {ECO:0000269|PubMed:25586105}. Cell surface
CC {ECO:0000269|PubMed:25586105}.
CC -!- INDUCTION: Expressed during infection. Expressed during exponential
CC growth in vitro. {ECO:0000269|PubMed:17328725}.
CC -!- BIOTECHNOLOGY: Immunodominant antigen that could be used for
CC serological diagnosis to distinguish patients with active tuberculosis
CC from M.bovis BCG-vaccinated individuals (PubMed:17328725,
CC PubMed:19467342, PubMed:23136116). Candidate for development of a
CC vaccine for the control of tuberculosis (PubMed:18426397,
CC PubMed:25586105). {ECO:0000269|PubMed:17328725,
CC ECO:0000269|PubMed:18426397, ECO:0000269|PubMed:19467342,
CC ECO:0000269|PubMed:23136116, ECO:0000269|PubMed:25586105}.
CC -!- SIMILARITY: Belongs to the mycobacterial PPE family. {ECO:0000305}.
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DR EMBL; AL123456; CCP46247.1; -; Genomic_DNA.
DR PIR; F70738; F70738.
DR RefSeq; WP_003916145.1; NZ_NVQJ01000027.1.
DR RefSeq; YP_177971.1; NC_000962.3.
DR AlphaFoldDB; Q50703; -.
DR SMR; Q50703; -.
DR STRING; 83332.Rv3425; -.
DR PaxDb; Q50703; -.
DR DNASU; 887635; -.
DR GeneID; 887635; -.
DR KEGG; mtu:Rv3425; -.
DR TubercuList; Rv3425; -.
DR eggNOG; COG5651; Bacteria.
DR PhylomeDB; Q50703; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0052572; P:response to host immune response; IBA:GO_Central.
DR Gene3D; 1.20.1260.20; -; 1.
DR InterPro; IPR000030; PPE_family.
DR InterPro; IPR038332; PPE_sf.
DR Pfam; PF00823; PPE; 1.
PE 1: Evidence at protein level;
KW Cell wall; Reference proteome; Secreted; Virulence.
FT CHAIN 1..176
FT /note="PPE family protein PPE57"
FT /id="PRO_0000217853"
SQ SEQUENCE 176 AA; 19855 MW; B8CEF2E9463B87B0 CRC64;
MHPMIPAEYI SNIIYEGPGA DSLFFASGQL RELAYSVETT AESLEDELDE LDENWKGSSS
DLLADAVERY LQWLSKHSSQ LKHAAWVING LANAYNDTRR KVVPPEEIAA NREERRRLIA
SNVAGVNTPA IADLDAQYDQ YRARNVAVMN AYVSWTRSAL SDLPRWREPP QIYRGG
