ID   PPF1_PEA                Reviewed;         442 AA.
AC   Q9FY06; O04699;
DT   14-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2003, sequence version 2.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Inner membrane protein PPF-1, chloroplastic;
DE   AltName: Full=Post-floral-specific protein 1;
DE   Flags: Precursor;
GN   Name=PPF-1;
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. G2; TISSUE=Apical bud;
RX   PubMed=9479033; DOI=10.1016/s0378-1119(97)00613-6;
RA   Zhu Y., Zhang Y., Luo J., Davies P.J., Ho D.T.-H.;
RT   "PPF-1, a post-floral-specific gene expressed in short-day-grown G2 pea,
RT   may be important for its never-senescing phenotype.";
RL   Gene 208:1-6(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND VARIANTS GLY-107; GLY-127; PRO-150; PRO-250
RP   AND 326-HIS-LYS-327.
RC   STRAIN=cv. Alaska;
RA   Xu Y., Wang M., Li Q., Ji X., Zhu Y.;
RT   "Cloning and sequencing of the homologous gene of PPF-1 from Alaska pea.";
RL   Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May be required for the insertion of some integral membrane
CC       proteins into the chloroplast thylakoid membrane. May play a role in
CC       inhibiting senescence.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC       {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in apical buds. Low levels of
CC       expression in leaves. Not expressed in roots, and stems.
CC   -!- DEVELOPMENTAL STAGE: Expressed after flower initiation.
CC   -!- INDUCTION: By the plant hormone gibberellin A3. Expression is induced
CC       to a greater extent by short day conditions than long day conditions.
CC   -!- SIMILARITY: Belongs to the OXA1/ALB3/YidC (TC 2.A.9.2) family.
CC       {ECO:0000305}.
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DR   EMBL; Y12618; CAA73179.1; -; mRNA.
DR   EMBL; AJ297606; CAC04249.1; -; mRNA.
DR   PIR; T06476; T06476.
DR   AlphaFoldDB; Q9FY06; -.
DR   SMR; Q9FY06; -.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0032977; F:membrane insertase activity; IEA:InterPro.
DR   InterPro; IPR028055; Membr_insert_YidC/Oxa1_C.
DR   InterPro; IPR001708; YidC/ALB3/OXA1/COX18.
DR   PANTHER; PTHR12428; PTHR12428; 1.
DR   Pfam; PF02096; 60KD_IMP; 1.
DR   TIGRFAMs; TIGR03592; yidC_oxa1_cterm; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Membrane; Plastid; Thylakoid; Transit peptide; Transmembrane;
KW   Transmembrane helix.
FT   TRANSIT         1..?
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..442
FT                   /note="Inner membrane protein PPF-1, chloroplastic"
FT                   /id="PRO_0000020370"
FT   TOPO_DOM        1..108
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        109..129
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        130..183
FT                   /note="Stromal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        184..204
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        205..296
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        297..317
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        318..442
FT                   /note="Stromal"
FT                   /evidence="ECO:0000255"
FT   REGION          350..371
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          390..442
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         107
FT                   /note="D -> G (in strain: cv. Alaska)"
FT                   /evidence="ECO:0000269|Ref.2"
FT   VARIANT         127
FT                   /note="V -> G (in strain: cv. Alaska)"
FT                   /evidence="ECO:0000269|Ref.2"
FT   VARIANT         150
FT                   /note="L -> P (in strain: cv. Alaska)"
FT                   /evidence="ECO:0000269|Ref.2"
FT   VARIANT         250
FT                   /note="L -> P (in strain: cv. Alaska)"
FT                   /evidence="ECO:0000269|Ref.2"
FT   VARIANT         326..327
FT                   /note="QQ -> HK (in strain: cv. Alaska)"
SQ   SEQUENCE   442 AA;  48238 MW;  3D4D4C99C5BE9B04 CRC64;
     MAKTLISSPS FLGTPLPSLH RTFSPNRTRL FTKVQFSFHQ LPPIQSVSHS VDLSGIFARA
     EGLLYTLADA TVAADAAAST DVAAQKNGGW FGFISDGMEF VLKVLKDGLS SVHVPYSYGF
     AIILLTVIVK AATLPLTKQQ VESTLAMQNL QPKIKAIQER YAGNQERIQL ETSRLYTQAG
     VNPLAGCLPT LATIPVWIGL YQALSNVANE GLLTEGFLWI PSLGGPTSIA ARQSGSGISW
     LFPFVDGHPL LGWYDTAAYL VLPVLLIVSQ YVSMEIMKPP QTNDPNQKNT LLIFKFLPLM
     IGYFSLSVPS GLTIYWFTNN VLSTAQQVWL RKLGGAKPAV NENAGGIITA GQAKRSASKP
     EKGGERFRQL KEEEKKKKLI KALPVEEVQP LASASASNDG SDVENNKEQE VTEESNTSKV
     SQEVQSFSRE RRSKRSKRKP VA