PPI2_ARATH
ID PPI2_ARATH Reviewed; 589 AA.
AC B3H4K7; B6EUC1; Q9LJR4;
DT 28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT 22-JUL-2008, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Proton pump-interactor 2;
GN Name=PPI2; OrderedLocusNames=At3g15340; ORFNames=K7L4.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12182706; DOI=10.1046/j.1365-313x.2002.01373.x;
RA Morandini P., Valera M., Albumi C., Bonza M.C., Giacometti S., Ravera G.,
RA Murgia I., Soave C., De Michelis M.I.;
RT "A novel interaction partner for the C-terminus of Arabidopsis thaliana
RT plasma membrane H+-ATPase (AHA1 isoform): site and mechanism of action on
RT H+-ATPase activity differ from those of 14-3-3 proteins.";
RL Plant J. 31:487-497(2002).
RN [4]
RP TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=18304198; DOI=10.1111/j.1438-8677.2007.00022.x;
RA Anzi C., Pelucchi P., Vazzola V., Murgia I., Gomarasca S., Piccoli M.B.,
RA Morandini P.;
RT "The proton pump interactor (Ppi) gene family of Arabidopsis thaliana:
RT expression pattern of Ppi1 and characterisation of knockout mutants for
RT Ppi1 and 2.";
RL Plant Biol. 10:237-249(2008).
CC -!- FUNCTION: May regulate plasma membrane ATPase activity. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass membrane
CC protein {ECO:0000250}. Endoplasmic reticulum membrane {ECO:0000250};
CC Single-pass membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=B3H4K7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=B3H4K7-2; Sequence=VSP_044423;
CC -!- TISSUE SPECIFICITY: Expressed in seedlings and flowers.
CC {ECO:0000269|PubMed:18304198}.
CC -!- SIMILARITY: Belongs to the plant Proton pump-interactor protein family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB02163.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP000413; BAB02163.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE75643.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75644.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64261.1; -; Genomic_DNA.
DR RefSeq; NP_001118633.1; NM_001125161.1. [B3H4K7-2]
DR RefSeq; NP_001326301.1; NM_001338153.1. [B3H4K7-1]
DR RefSeq; NP_188152.3; NM_112397.4. [B3H4K7-1]
DR AlphaFoldDB; B3H4K7; -.
DR SMR; B3H4K7; -.
DR STRING; 3702.AT3G15340.1; -.
DR PaxDb; B3H4K7; -.
DR PRIDE; B3H4K7; -.
DR ProteomicsDB; 249346; -. [B3H4K7-1]
DR EnsemblPlants; AT3G15340.1; AT3G15340.1; AT3G15340. [B3H4K7-1]
DR EnsemblPlants; AT3G15340.2; AT3G15340.2; AT3G15340. [B3H4K7-2]
DR EnsemblPlants; AT3G15340.3; AT3G15340.3; AT3G15340. [B3H4K7-1]
DR GeneID; 820765; -.
DR Gramene; AT3G15340.1; AT3G15340.1; AT3G15340. [B3H4K7-1]
DR Gramene; AT3G15340.2; AT3G15340.2; AT3G15340. [B3H4K7-2]
DR Gramene; AT3G15340.3; AT3G15340.3; AT3G15340. [B3H4K7-1]
DR KEGG; ath:AT3G15340; -.
DR Araport; AT3G15340; -.
DR TAIR; locus:2087115; AT3G15340.
DR eggNOG; ENOG502QQX1; Eukaryota.
DR InParanoid; B3H4K7; -.
DR OMA; YKMVFEE; -.
DR PhylomeDB; B3H4K7; -.
DR PRO; PR:B3H4K7; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; B3H4K7; baseline and differential.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0010155; P:regulation of proton transport; ISS:UniProtKB.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Coiled coil; Endoplasmic reticulum;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..589
FT /note="Proton pump-interactor 2"
FT /id="PRO_0000420213"
FT TRANSMEM 568..588
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 370..450
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 485..534
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 205..245
FT /evidence="ECO:0000255"
FT COILED 431..500
FT /evidence="ECO:0000255"
FT COMPBIAS 394..450
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 507..534
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..120
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_044423"
SQ SEQUENCE 589 AA; 67324 MW; 5CB13AF9D7AC2263 CRC64;
MGAQIILSEG FEVVPPPEMN DLVLFGSNQS VSSCDVSTVT TEDGTVFSGD SSPGGATEED
FPEEKPFSFY FVKQPAYDDP EIKAKIDEAG HEINRYNKDR IVVSNAQESE KAEILSLFGQ
MKSLVSKSEG YRVVIEEKKM EFDALHESLR NLRCSTSDQL CFSKEELDHL IYIAHYQIEH
GSIGFEEEDW VLKETEKADG IVLSEDSLAE KEASINRVKS MAVELNEVKK ELDAITWKIN
HLSDKVGKSQ NNLRVLDVKK AHILEERDRS YERIKMLRIQ RDKGKAAFYQ SLPVMRKARE
LAASGNVRDL EVFASSEADR FMTQWNDDKA FRDDYVKRIS PSLCERQLNQ DGRIKDPEVQ
VVWEKKVPVK GGEKVHETNR EDSSSNSSQY GSVITDKRKK ETRKKAMDFN RSSAEESDVT
DLEFSVYEKP KKEEEEVDEE TLKEREREEQ LEKARLAMER KRKLQEKAAA KAAIRAQKEA
EKKLKECEKK AKKKAAANSS SPSESDHSQE VTKDLEKVRT LAVSGKEKHQ KERSLFPKQR
SFRYKHRGRG TEALPKAILN RRKAHKYWVW GLSSAALAVA LFLVVLLLR
