PPK19_DROME
ID PPK19_DROME Reviewed; 511 AA.
AC Q9VAJ3; Q86LG6;
DT 31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Pickpocket protein 19;
DE Short=PPK19 {ECO:0000303|PubMed:12571352, ECO:0000312|EMBL:AAO47373.1};
GN Name=ppk19; ORFNames=CG18287;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAO47373.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12571352; DOI=10.1073/pnas.252785099;
RA Liu L., Johnson W.A., Welsh M.J.;
RT "Drosophila DEG/ENaC pickpocket genes are expressed in the tracheal system,
RT where they may be involved in liquid clearance.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:2128-2133(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12848938; DOI=10.1016/s0896-6273(03)00394-5;
RA Liu L., Leonard A.S., Motto D.G., Feller M.A., Price M.P., Johnson W.A.,
RA Welsh M.J.;
RT "Contribution of Drosophila DEG/ENaC genes to salt taste.";
RL Neuron 39:133-146(2003).
CC -!- FUNCTION: Part of a complex that plays a role in tracheal liquid
CC clearance. In both larvae and adults, contributes to the behavioral
CC response to salt. Probable role in sodium transport.
CC {ECO:0000269|PubMed:12848938, ECO:0000303|PubMed:12571352,
CC ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in the tracheal system. Expressed in the
CC taste-sensing terminal organ of the larval head. In adults, expressed
CC in hairs on the tibia, femur and wing margin, but not in hairs on the
CC tarsi of the leg. {ECO:0000269|PubMed:12571352,
CC ECO:0000269|PubMed:12848938}.
CC -!- SIMILARITY: Belongs to the amiloride-sensitive sodium channel (TC
CC 1.A.6) family. {ECO:0000305}.
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DR EMBL; AY226547; AAO47373.1; -; mRNA.
DR EMBL; AE014297; AAF56913.2; -; Genomic_DNA.
DR RefSeq; NP_651708.2; NM_143451.2.
DR AlphaFoldDB; Q9VAJ3; -.
DR IntAct; Q9VAJ3; 4.
DR STRING; 7227.FBpp0084817; -.
DR PaxDb; Q9VAJ3; -.
DR DNASU; 43489; -.
DR EnsemblMetazoa; FBtr0085451; FBpp0084817; FBgn0039679.
DR GeneID; 43489; -.
DR KEGG; dme:Dmel_CG18287; -.
DR UCSC; CG18287-RA; d. melanogaster.
DR CTD; 43489; -.
DR FlyBase; FBgn0039679; ppk19.
DR VEuPathDB; VectorBase:FBgn0039679; -.
DR eggNOG; KOG4294; Eukaryota.
DR HOGENOM; CLU_024950_3_1_1; -.
DR InParanoid; Q9VAJ3; -.
DR OMA; QTYLNVR; -.
DR OrthoDB; 686369at2759; -.
DR PhylomeDB; Q9VAJ3; -.
DR Reactome; R-DME-2672351; Stimuli-sensing channels.
DR BioGRID-ORCS; 43489; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 43489; -.
DR PRO; PR:Q9VAJ3; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0039679; Expressed in tracheal tip cell (Drosophila) and 7 other tissues.
DR Genevisible; Q9VAJ3; DM.
DR GO; GO:0016021; C:integral component of membrane; ISS:FlyBase.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0015280; F:ligand-gated sodium channel activity; IBA:GO_Central.
DR GO; GO:0005272; F:sodium channel activity; ISS:FlyBase.
DR GO; GO:0035002; P:liquid clearance, open tracheal system; NAS:UniProtKB.
DR GO; GO:0009651; P:response to salt stress; IMP:UniProtKB.
DR GO; GO:0035199; P:salt aversion; IMP:UniProtKB.
DR GO; GO:0050914; P:sensory perception of salty taste; IMP:FlyBase.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006814; P:sodium ion transport; ISS:FlyBase.
DR InterPro; IPR001873; ENaC.
DR PANTHER; PTHR11690; PTHR11690; 1.
DR Pfam; PF00858; ASC; 1.
DR PRINTS; PR01078; AMINACHANNEL.
PE 2: Evidence at transcript level;
KW Ion channel; Ion transport; Membrane; Reference proteome; Sodium;
KW Sodium channel; Sodium transport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..511
FT /note="Pickpocket protein 19"
FT /id="PRO_0000420126"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 471..491
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 112
FT /note="N -> S (in Ref. 1; AAO47373)"
FT /evidence="ECO:0000305"
FT CONFLICT 158
FT /note="R -> L (in Ref. 1; AAO47373)"
FT /evidence="ECO:0000305"
FT CONFLICT 230
FT /note="K -> N (in Ref. 1; AAO47373)"
FT /evidence="ECO:0000305"
FT CONFLICT 247
FT /note="D -> E (in Ref. 1; AAO47373)"
FT /evidence="ECO:0000305"
FT CONFLICT 259
FT /note="S -> A (in Ref. 1; AAO47373)"
FT /evidence="ECO:0000305"
FT CONFLICT 357
FT /note="Q -> R (in Ref. 1; AAO47373)"
FT /evidence="ECO:0000305"
FT CONFLICT 418
FT /note="R -> Q (in Ref. 1; AAO47373)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 511 AA; 59461 MW; DF8668C68E5CCACA CRC64;
MLLYTKELVI PRPRPGLLRF RNNPRGIKFR EKLCNSFAHS NIHGMQHVFG EQHLWQRCLW
LAIVLGAVIT GFSLYTVLMH RHSEQLLVSL IETTQLPVYH IDFPAVAVCP WNHFNWQRAP
SAFIRFLPRH PNAELRETFR QLLASMDIMN FSNFNRIRIL TKRNLTGISY LKMTDLMNFM
TYRCDELFVA DSCVFDETPY DCCKLFVREQ TVKGQCLVFN SMISENSRKK HLINQFYPHK
LSTAGEDSGL KFTINASYSF MNNIDALTPF GMNLMIKEPR QWSNEMMYHL YPDTENFVAV
HPLVTETSPN TYEMSPKKRR CYFDDEKNPT FQNTSLTYNR ENCLVVCLHL VVWKTCQCSL
PAFLPPIDGV PECGINDAQC LGNNSDIFTY VKMGDQEKYI NDSRQGHFCD CPDNCNSRLY
EMSLNVRKLD YPKNSTDQLI KAQVYYGQRV MTKIITKLKY TNIDLLANFG GIISLYIGAS
VMSFIELLFV LGKLMWGFIR DARIKLKEYT K
