PPK1_ACIBA
ID PPK1_ACIBA Reviewed; 692 AA.
AC Q9X4M8;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=Polyphosphate kinase {ECO:0000255|HAMAP-Rule:MF_00347};
DE EC=2.7.4.1 {ECO:0000255|HAMAP-Rule:MF_00347};
DE AltName: Full=ATP-polyphosphate phosphotransferase {ECO:0000255|HAMAP-Rule:MF_00347};
DE AltName: Full=Polyphosphoric acid kinase {ECO:0000255|HAMAP-Rule:MF_00347};
GN Name=ppk {ECO:0000255|HAMAP-Rule:MF_00347};
OS Acinetobacter baumannii.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Moraxellales; Moraxellaceae;
OC Acinetobacter; Acinetobacter calcoaceticus/baumannii complex.
OX NCBI_TaxID=470;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=252;
RX PubMed=10537215; DOI=10.1099/00221287-145-10-2931;
RA Gavigan J.A., Marshall L.M., Dobson A.D.W.;
RT "Regulation of polyphosphate kinase gene expression in Acinetobacter
RT baumannii 252.";
RL Microbiology 145:2931-2937(1999).
CC -!- FUNCTION: Catalyzes the reversible transfer of the terminal phosphate
CC of ATP to form a long-chain polyphosphate (polyP). {ECO:0000255|HAMAP-
CC Rule:MF_00347}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[phosphate](n) + ATP = [phosphate](n+1) + ADP;
CC Xref=Rhea:RHEA:19573, Rhea:RHEA-COMP:9859, Rhea:RHEA-COMP:14280,
CC ChEBI:CHEBI:16838, ChEBI:CHEBI:30616, ChEBI:CHEBI:456216; EC=2.7.4.1;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00347};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00347};
CC -!- PTM: An intermediate of this reaction is the autophosphorylated ppk in
CC which a phosphate is covalently linked to a histidine residue through a
CC N-P bond. {ECO:0000255|HAMAP-Rule:MF_00347}.
CC -!- SIMILARITY: Belongs to the polyphosphate kinase 1 (PPK1) family.
CC {ECO:0000255|HAMAP-Rule:MF_00347}.
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DR EMBL; AF116175; AAD28429.1; -; Genomic_DNA.
DR AlphaFoldDB; Q9X4M8; -.
DR SMR; Q9X4M8; -.
DR GO; GO:0009358; C:polyphosphate kinase complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008976; F:polyphosphate kinase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0006799; P:polyphosphate biosynthetic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.1840.10; -; 1.
DR HAMAP; MF_00347; Polyphosphate_kinase; 1.
DR InterPro; IPR003414; PP_kinase.
DR InterPro; IPR041108; PP_kinase_C_1.
DR InterPro; IPR024953; PP_kinase_middle.
DR InterPro; IPR036830; PP_kinase_middle_dom_sf.
DR InterPro; IPR025200; PPK_C_dom2.
DR InterPro; IPR025198; PPK_N_dom.
DR InterPro; IPR036832; PPK_N_dom_sf.
DR PANTHER; PTHR30218; PTHR30218; 1.
DR Pfam; PF02503; PP_kinase; 1.
DR Pfam; PF13090; PP_kinase_C; 1.
DR Pfam; PF17941; PP_kinase_C_1; 1.
DR Pfam; PF13089; PP_kinase_N; 1.
DR PIRSF; PIRSF015589; PP_kinase; 1.
DR SUPFAM; SSF140356; SSF140356; 1.
DR TIGRFAMs; TIGR03705; poly_P_kin; 1.
PE 3: Inferred from homology;
KW ATP-binding; Kinase; Magnesium; Metal-binding; Nucleotide-binding;
KW Phosphoprotein; Transferase.
FT CHAIN 1..692
FT /note="Polyphosphate kinase"
FT /id="PRO_0000128631"
FT ACT_SITE 443
FT /note="Phosphohistidine intermediate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00347"
FT BINDING 57
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00347"
FT BINDING 383
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00347"
FT BINDING 413
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00347"
FT BINDING 476
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00347"
FT BINDING 572
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00347"
FT BINDING 600
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00347"
SQ SEQUENCE 692 AA; 78980 MW; C7EB0011DF332920 CRC64;
MNSVVTQASP PVYGQVERYI NRELSILDFN LRVLEQAVDP LNPLLEKLNF LLIFSRNLDE
FFEIRVATML EHFVQERDIQ TADGLSPQEI LHQISETAHA AIERQYQILN EQIFPQLREE
GISFLRRGEL TQAQSNWVKK YFQEQVAPAL TPISLDPAHP FPRLVNKSLN FIVTLEGKDA
FGRQIDLAVV PAPHSLPRVV RLPDELTEGK EHHVMLSSII HTHVSDSFPG MTATGCYQFR
VTRNADLTLT EDVEDLAEAL KDELSSRRFG RAVRLEVNKN CPTHIYEYLL KEFDLDTHQL
YRVDGPVNLA RLVSDFKRPY LRYEPHTPVI PKILKKSANI FSAMQKQDIL LHHPFESFSP
VIRLLREAAQ DPHVLAIKQT LYRSGANSEI VQILAEAARN GKEVTAVIEL RARFDEESNI
AVANVLQEAG AVVVYGIVGY KTHAKMILVV RRENQKLVRY AHLGTGNYHA GNAKIYTDYG
LLTTNQDLCE DVHRIFQELT GMGKMVKLKK LLHAPFTLHS QLMNFIENEI VLAKAGKPAK
IIIKVNALTE VQLINKLYEA SQAGVKIELI IRSICCLRPG LAGLSENIRV RSIVGRFLEH
TRVYYFYNNG DTRVYCSSAD WMERNLFKRV EVCLPIENTG LKKRIYQQGL ENYLMDNQQS
WLLQSDGSWC RNISTEDQKP HNAQQMLLGD II