ATF1_MOUSE
ID ATF1_MOUSE Reviewed; 269 AA.
AC P81269;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 1.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Cyclic AMP-dependent transcription factor ATF-1;
DE Short=cAMP-dependent transcription factor ATF-1;
DE AltName: Full=Activating transcription factor 1;
DE AltName: Full=TCR-ATF1;
GN Name=Atf1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND SUBCELLULAR LOCATION.
RX PubMed=1531847;
RA Lee M.-R., Chung C.-S., Liou M.-L., Wu M., Li W.-F., Hsueh Y.-P.,
RA Lai M.-Z.;
RT "Isolation and characterization of nuclear proteins that bind to T cell
RT receptor V beta decamer motif.";
RL J. Immunol. 148:1906-1912(1992).
RN [2]
RP PHOSPHORYLATION AT SER-63.
RX PubMed=18690222; DOI=10.1038/ni.1644;
RA Ananieva O., Darragh J., Johansen C., Carr J.M., McIlrath J., Park J.M.,
RA Wingate A., Monk C.E., Toth R., Santos S.G., Iversen L., Arthur J.S.;
RT "The kinases MSK1 and MSK2 act as negative regulators of Toll-like receptor
RT signaling.";
RL Nat. Immunol. 9:1028-1036(2008).
CC -!- FUNCTION: Binds the cAMP response element (CRE) (consensus: 5'-
CC GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular
CC promoters. Binds to the Tax-responsive element (TRE) of HTLV-I.
CC Mediates PKA-induced stimulation of CRE-reporter genes. Represses the
CC expression of FTH1 and other antioxidant detoxification genes. Triggers
CC cell proliferation and transformation (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Binds DNA as a dimer. Interacts with HIPK2 and CDK3. Interacts
CC with MOTS-c, a peptide produced by the mitochondrially encoded 12S rRNA
CC MT-RNR1; the interaction occurs in the nucleus following metabolic
CC stress. {ECO:0000250|UniProtKB:P18846}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:1531847}.
CC -!- PTM: Phosphorylated at Ser-196 by HIPK2 in response to genotoxic
CC stress. This phosphorylation promotes transcription repression of FTH1
CC and other antioxidant detoxification genes. The CDK3-mediated
CC phosphorylation at Ser-63 promotes its transactivation and
CC transcriptional activities (By similarity). Phosphorylated at Ser-63 by
CC RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli.
CC {ECO:0000250, ECO:0000269|PubMed:18690222}.
CC -!- SIMILARITY: Belongs to the bZIP family. ATF subfamily. {ECO:0000305}.
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DR EMBL; M63725; AAA40395.1; -; mRNA.
DR CCDS; CCDS27832.1; -.
DR PIR; A46490; A46490.
DR RefSeq; NP_031523.3; NM_007497.3.
DR RefSeq; XP_006520414.1; XM_006520351.3.
DR RefSeq; XP_006520415.1; XM_006520352.3.
DR AlphaFoldDB; P81269; -.
DR BioGRID; 198232; 2.
DR ComplexPortal; CPX-7; bZIP transcription factor complex, Atf1-Atf4.
DR IntAct; P81269; 2.
DR STRING; 10090.ENSMUSP00000023769; -.
DR iPTMnet; P81269; -.
DR PhosphoSitePlus; P81269; -.
DR MaxQB; P81269; -.
DR PaxDb; P81269; -.
DR PeptideAtlas; P81269; -.
DR PRIDE; P81269; -.
DR ProteomicsDB; 265141; -.
DR Antibodypedia; 4260; 700 antibodies from 43 providers.
DR DNASU; 11908; -.
DR Ensembl; ENSMUST00000023769; ENSMUSP00000023769; ENSMUSG00000023027.
DR GeneID; 11908; -.
DR KEGG; mmu:11908; -.
DR UCSC; uc007xqs.1; mouse.
DR CTD; 466; -.
DR MGI; MGI:1298366; Atf1.
DR VEuPathDB; HostDB:ENSMUSG00000023027; -.
DR eggNOG; KOG3584; Eukaryota.
DR GeneTree; ENSGT00940000158200; -.
DR HOGENOM; CLU_042675_1_0_1; -.
DR InParanoid; P81269; -.
DR OMA; THISHIA; -.
DR OrthoDB; 957343at2759; -.
DR PhylomeDB; P81269; -.
DR TreeFam; TF106464; -.
DR Reactome; R-MMU-199920; CREB phosphorylation.
DR BioGRID-ORCS; 11908; 3 hits in 76 CRISPR screens.
DR ChiTaRS; Atf1; mouse.
DR PRO; PR:P81269; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; P81269; protein.
DR Bgee; ENSMUSG00000023027; Expressed in cumulus cell and 264 other tissues.
DR ExpressionAtlas; P81269; baseline and differential.
DR Genevisible; P81269; MM.
DR GO; GO:1990590; C:ATF1-ATF4 transcription factor complex; IDA:ParkinsonsUK-UCL.
DR GO; GO:1990589; C:ATF4-CREB1 transcription factor complex; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IPI:ComplexPortal.
DR GO; GO:0005667; C:transcription regulator complex; IDA:MGI.
DR GO; GO:0003677; F:DNA binding; IDA:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:InterPro.
DR GO; GO:0044877; F:protein-containing complex binding; ISO:MGI.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISO:MGI.
DR GO; GO:0110148; P:biomineralization; ISO:MGI.
DR GO; GO:0019933; P:cAMP-mediated signaling; ISO:MGI.
DR GO; GO:0042789; P:mRNA transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0045740; P:positive regulation of DNA replication; ISO:MGI.
DR GO; GO:0010976; P:positive regulation of neuron projection development; ISO:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:ComplexPortal.
DR GO; GO:0065003; P:protein-containing complex assembly; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0032025; P:response to cobalt ion; IEA:Ensembl.
DR GO; GO:0010035; P:response to inorganic substance; IMP:MGI.
DR GO; GO:0014074; P:response to purine-containing compound; ISO:MGI.
DR InterPro; IPR029825; ATF1.
DR InterPro; IPR004827; bZIP.
DR InterPro; IPR046347; bZIP_sf.
DR InterPro; IPR003102; Coactivator_CBP_pKID.
DR InterPro; IPR001630; Leuzip_CREB.
DR PANTHER; PTHR45879; PTHR45879; 1.
DR PANTHER; PTHR45879:SF2; PTHR45879:SF2; 1.
DR Pfam; PF00170; bZIP_1; 1.
DR Pfam; PF02173; pKID; 1.
DR SMART; SM00338; BRLZ; 1.
DR SUPFAM; SSF57959; SSF57959; 1.
DR PROSITE; PS50217; BZIP; 1.
DR PROSITE; PS00036; BZIP_BASIC; 1.
DR PROSITE; PS50953; KID; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation;
KW Ubl conjugation.
FT CHAIN 1..269
FT /note="Cyclic AMP-dependent transcription factor ATF-1"
FT /id="PRO_0000076576"
FT DOMAIN 31..90
FT /note="KID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00312"
FT DOMAIN 211..269
FT /note="bZIP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 1..90
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 213..237
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 239..260
FT /note="Leucine-zipper"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT COMPBIAS 8..52
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 63..82
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 63
FT /note="Phosphoserine; by CaMK1, CDK3, RPS6KA4 and RPS6KA5"
FT /evidence="ECO:0000305|PubMed:18690222"
FT MOD_RES 196
FT /note="Phosphoserine; by HIPK2"
FT /evidence="ECO:0000250|UniProtKB:P18846,
FT ECO:0000255|PROSITE-ProRule:PRU00312"
FT CROSSLNK 206
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:P18846"
SQ SEQUENCE 269 AA; 29238 MW; 9885265159D64A0C CRC64;
MEDSHKSNTT ETASQPGSTV AGPHVSQIVH QVSSLSESEE SQDSSDSIGS SQKAHGILAR
RPSYRKILKD LSSEDTRGRK GEGENPSISA ITSMSVPAPI YQTSSGQYIA IAPNGALQLA
SPSTDGVQAL QTLTMTNSSS TQQGTILQYA QTSDGQQILV PSNQVVVQTA SGDMQTYQIR
TTPSATSLPQ TVVMTSPVTL ASQTTKTDDP QLRREIRLMK NREAARECRR KKKEYVKCLE
NRVAVLENQN KTLIEELKTL KDLYSHKSV
