ATF1_SCHPO
ID ATF1_SCHPO Reviewed; 566 AA.
AC P52890; P78944;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Transcription factor atf1;
DE AltName: Full=Protein sss1;
DE AltName: Full=Transcription factor mts1;
GN Name=atf1; Synonyms=gad7, mts1, sss1; ORFNames=SPBC29B5.01;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=8557039; DOI=10.1002/j.1460-2075.1995.tb00310.x;
RA Takeda T., Toda T., Kominami K., Kohnosu A., Yanagida M., Jones N.;
RT "Schizosaccharomyces pombe atf1+ encodes a transcription factor required
RT for sexual development and entry into stationary phase.";
RL EMBO J. 14:6193-6208(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PHOSPHORYLATION BY STY1/SPC1.
RX PubMed=8824587; DOI=10.1101/gad.10.18.2276;
RA Shiozaki K., Russell P.;
RT "Conjugation, meiosis, and the osmotic stress response are regulated by
RT Spc1 kinase through Atf1 transcription factor in fission yeast.";
RL Genes Dev. 10:2276-2288(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBUNIT.
RX PubMed=9391101; DOI=10.1073/pnas.94.25.13765;
RA Kon N., Krawchuk M.D., Warren B.G., Smith G.R., Wahls W.P.;
RT "Transcription factor Mts1/Mts2 (Atf1/Pcr1, Gad7/Pcr1) activates the M26
RT meiotic recombination hotspot in Schizosaccharomyces pombe.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:13765-13770(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9135083; DOI=10.1046/j.1365-2443.1996.d01-247.x;
RA Kanoh J., Watanabe Y., Ohsugi M., Iino Y., Yamamoto M.;
RT "Schizosaccharomyces pombe gad7+ encodes a phosphoprotein with a bZIP
RT domain, which is required for proper G1 arrest and gene expression under
RT nitrogen starvation.";
RL Genes Cells 1:391-408(1996).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RA Kohnosu A., Niwa O., Yano M., Saitoh S., Katayama T., Nagao K.,
RA Yanagida M.;
RT "S.pombe chromosome II cosmid 1228 sequence.";
RL Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [7]
RP CHARACTERIZATION.
RX PubMed=7958849; DOI=10.1101/gad.8.14.1693;
RA Wahls W.P., Smith G.R.;
RT "A heteromeric protein that binds to a meiotic homologous recombination hot
RT spot: correlation of binding and hot spot activity.";
RL Genes Dev. 8:1693-1702(1994).
RN [8]
RP DISRUPTION PHENOTYPE.
RX PubMed=28775286; DOI=10.1038/s41598-017-07647-1;
RA Villahermosa D., Fleck O.;
RT "Elp3 and Dph3 of Schizosaccharomyces pombe mediate cellular stress
RT responses through tRNALysUUU modifications.";
RL Sci. Rep. 7:7225-7225(2017).
CC -!- FUNCTION: Transcription factor required for sexual development and
CC entry into stationary phase. Binds and activates CRE sites (cAMP-
CC response elements, also known as M26 meiotic recombination hotspots).
CC {ECO:0000269|PubMed:9391101}.
CC -!- SUBUNIT: Heterodimer of pcr1/mts2 and atf1/mts1.
CC {ECO:0000269|PubMed:9391101}.
CC -!- INTERACTION:
CC P52890; Q09892: sty1; NbExp=2; IntAct=EBI-3861527, EBI-3648525;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- PTM: Phosphorylated by sty1/spc1. {ECO:0000269|PubMed:8824587}.
CC -!- DISRUPTION PHENOTYPE: Sensitive to methyl methanesulfonate (MMS, causes
CC DNA breaks), hydroxyurea (HU, ribonucleotide reductase inhibitor),
CC thiabendazole (TBZ), sirolimus (TORC1 inhibitor), and cold
CC (PubMed:28775286). Simultaneous disruption of dph3 exacerbates
CC sensitivity to HU and MMS (PubMed:28775286).
CC {ECO:0000269|PubMed:28775286}.
CC -!- SIMILARITY: Belongs to the bZIP family. {ECO:0000305}.
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DR EMBL; D63762; BAA09841.1; -; Genomic_DNA.
DR EMBL; U38237; AAA93260.1; -; Genomic_DNA.
DR EMBL; U87869; AAB46990.1; -; Genomic_DNA.
DR EMBL; D63666; BAA09817.1; -; Genomic_DNA.
DR EMBL; D83992; BAA12194.1; -; Genomic_DNA.
DR EMBL; CU329671; CAC05510.1; -; Genomic_DNA.
DR PIR; S66147; S66147.
DR RefSeq; NP_595652.1; NM_001021546.2.
DR AlphaFoldDB; P52890; -.
DR SMR; P52890; -.
DR BioGRID; 276859; 47.
DR IntAct; P52890; 1.
DR STRING; 4896.SPBC29B5.01.1; -.
DR iPTMnet; P52890; -.
DR MaxQB; P52890; -.
DR PaxDb; P52890; -.
DR PRIDE; P52890; -.
DR EnsemblFungi; SPBC29B5.01.1; SPBC29B5.01.1:pep; SPBC29B5.01.
DR PomBase; SPBC29B5.01; atf1.
DR VEuPathDB; FungiDB:SPBC29B5.01; -.
DR eggNOG; KOG1414; Eukaryota.
DR HOGENOM; CLU_435572_0_0_1; -.
DR InParanoid; P52890; -.
DR OMA; HGAFMSQ; -.
DR PhylomeDB; P52890; -.
DR Reactome; R-SPO-3214847; HATs acetylate histones.
DR Reactome; R-SPO-450341; Activation of the AP-1 family of transcription factors.
DR PRO; PR:P52890; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:1990243; C:atf1-pcr1 complex; IDA:PomBase.
DR GO; GO:0000785; C:chromatin; IDA:PomBase.
DR GO; GO:0000792; C:heterochromatin; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; IDA:PomBase.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:PomBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0003690; F:double-stranded DNA binding; IDA:PomBase.
DR GO; GO:0010844; F:recombination hotspot binding; IDA:PomBase.
DR GO; GO:0003723; F:RNA binding; IDA:PomBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:PomBase.
DR GO; GO:0010846; P:activation of reciprocal meiotic recombination; IMP:PomBase.
DR GO; GO:0034599; P:cellular response to oxidative stress; IMP:PomBase.
DR GO; GO:0006325; P:chromatin organization; IMP:PomBase.
DR GO; GO:0110034; P:negative regulation of adenylate cyclase-activating glucose-activated G protein-coupled receptor signaling pathway; IMP:PomBase.
DR GO; GO:0060195; P:negative regulation of antisense RNA transcription; IMP:PomBase.
DR GO; GO:0045128; P:negative regulation of reciprocal meiotic recombination; IMP:PomBase.
DR GO; GO:0061417; P:negative regulation of transcription from RNA polymerase II promoter in response to oxidative stress; IMP:PomBase.
DR GO; GO:1904765; P:positive regulation of transcription from RNA polymerase II promoter in response to maltose; IMP:PomBase.
DR GO; GO:0036091; P:positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress; IMP:PomBase.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:PomBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR004827; bZIP.
DR InterPro; IPR046347; bZIP_sf.
DR InterPro; IPR021755; TF_Aft1_HRA.
DR InterPro; IPR020956; TF_Aft1_OSM.
DR Pfam; PF11786; Aft1_HRA; 1.
DR Pfam; PF11785; Aft1_OSA; 1.
DR Pfam; PF00170; bZIP_1; 1.
DR SMART; SM00338; BRLZ; 1.
DR SUPFAM; SSF57959; SSF57959; 1.
DR PROSITE; PS50217; BZIP; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Meiosis; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..566
FT /note="Transcription factor atf1"
FT /id="PRO_0000076533"
FT DOMAIN 472..535
FT /note="bZIP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 1..83
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 96..117
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 315..345
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 357..479
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 474..503
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 514..528
FT /note="Leucine-zipper"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT COMPBIAS 1..79
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 375..463
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 464..479
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 566 AA; 59711 MW; 26DBFA9F967EFCF2 CRC64;
MSPSPVNTST EPASVAAVSN GNATASSTQV PENNQSDSFA PPSNNSQQNQ QSSTIAPNGG
AGSVANANPA DQSDGVTPSF VGSLKLDYEP NPFEHSFGST ASVGQGNPSL NRNPSLSNIP
SGVPPAFART LLPPVSSIAS PDILSGAPGI ASPLGYPAWS AFTRGTMHNP LSPAIYDATL
RPDYLNNPSD ASAAARFSSG TGFTPGVNEP FRSLLTPTGA GFPAPSPGTA NLLGFHTFDS
QFPDQYRFTP RDGKPPVVNG TNGDQSDYFG ANAAVHGLCL LSQVPDQQQK LQQPISSEND
QAASTTANNL LKQTQQQTFP DSIRPSFTQN TNPQAVTGTM NPQASRTQQQ PMYFMGSQQF
NGMPSVYGDT VNPADPSLTL RQTTDFSGQN AENGSTNLPQ KTSNSDMPTA NSMPVKLENG
TDYSTSQEPS SNANNQSSPT SSINGKASSE SANGTSYSKG SSRRNSKNET DEEKRKSFLE
RNRQAALKCR QRKKQWLSNL QAKVEFYGNE NEILSAQVSA LREEIVSLKT LLIAHKDCPV
AKSNSAAVAT SVIGSGDLAQ RINLGY
