ATF1_YEAST
ID ATF1_YEAST Reviewed; 525 AA.
AC P40353; D6W370; Q08901;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Alcohol O-acetyltransferase 1 {ECO:0000303|PubMed:8085822};
DE Short=AATase 1 {ECO:0000303|PubMed:8085822};
DE EC=2.3.1.84 {ECO:0000269|PubMed:28160314, ECO:0000269|PubMed:7764365};
DE EC=3.1.2.20 {ECO:0000269|PubMed:28160314};
GN Name=ATF1 {ECO:0000303|PubMed:8085822}; OrderedLocusNames=YOR377W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC STRAIN=Kyokai No.7;
RX PubMed=8085822; DOI=10.1128/aem.60.8.2786-2792.1994;
RA Fujii T., Nagasawa N., Iwamatsu A., Bogaki T., Tamai Y., Hamachi M.;
RT "Molecular cloning, sequence analysis, and expression of the yeast alcohol
RT acetyltransferase gene.";
RL Appl. Environ. Microbiol. 60:2786-2792(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP PARTIAL PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL
RP PROPERTIES, AND ACTIVITY REGULATION.
RX PubMed=7764365; DOI=10.1271/bbb.57.2094;
RA Minetoki T., Bogaki T., Iwamatsu A., Fujii T., Hamachi M.;
RT "The purification, properties and internal peptide sequences of alcohol
RT acetyltransferase isolated from Saccharomyces cerevisiae Kyokai No. 7.";
RL Biosci. Biotechnol. Biochem. 57:2094-2098(1993).
RN [5]
RP INDUCTION.
RX PubMed=9055409; DOI=10.1128/aem.63.3.910-915.1997;
RA Fujii T., Kobayashi O., Yoshimoto H., Furukawa S., Tamai Y.;
RT "Effect of aeration and unsaturated fatty acids on expression of the
RT Saccharomyces cerevisiae alcohol acetyltransferase gene.";
RL Appl. Environ. Microbiol. 63:910-915(1997).
RN [6]
RP FUNCTION.
RX PubMed=9758847; DOI=10.1128/aem.64.10.4076-4078.1998;
RA Fukuda K., Yamamoto N., Kiyokawa Y., Yanagiuchi T., Wakai Y., Kitamoto K.,
RA Inoue Y., Kimura A.;
RT "Balance of activities of alcohol acetyltransferase and esterase in
RT Saccharomyces cerevisiae is important for production of isoamyl acetate.";
RL Appl. Environ. Microbiol. 64:4076-4078(1998).
RN [7]
RP INDUCTION.
RX PubMed=9675816;
RX DOI=10.1002/(sici)1097-0061(19980615)14:8<711::aid-yea263>3.0.co;2-8;
RA Fujiwara D., Yoshimoto H., Sone H., Harashima S., Tamai Y.;
RT "Transcriptional co-regulation of Saccharomyces cerevisiae alcohol
RT acetyltransferase gene, ATF1 and delta-9 fatty acid desaturase gene, OLE1
RT by unsaturated fatty acids.";
RL Yeast 14:711-721(1998).
RN [8]
RP INDUCTION.
RX PubMed=10487921;
RX DOI=10.1002/(sici)1097-0061(19990915)15:12<1183::aid-yea444>3.0.co;2-j;
RA Fujiwara D., Kobayashi O., Yoshimoto H., Harashima S., Tamai Y.;
RT "Molecular mechanism of the multiple regulation of the Saccharomyces
RT cerevisiae ATF1 gene encoding alcohol acetyltransferase.";
RL Yeast 15:1183-1197(1999).
RN [9]
RP FUNCTION, AND BIOTECHNOLOGY.
RX PubMed=10653746; DOI=10.1128/aem.66.2.744-753.2000;
RA Lilly M., Lambrechts M.G., Pretorius I.S.;
RT "Effect of increased yeast alcohol acetyltransferase activity on flavor
RT profiles of wine and distillates.";
RL Appl. Environ. Microbiol. 66:744-753(2000).
RN [10]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=12957907; DOI=10.1128/aem.69.9.5228-5237.2003;
RA Verstrepen K.J., Van Laere S.D., Vanderhaegen B.M., Derdelinckx G.,
RA Dufour J.P., Pretorius I.S., Winderickx J., Thevelein J.M., Delvaux F.R.;
RT "Expression levels of the yeast alcohol acetyltransferase genes ATF1, Lg-
RT ATF1, and ATF2 control the formation of a broad range of volatile esters.";
RL Appl. Environ. Microbiol. 69:5228-5237(2003).
RN [11]
RP INDUCTION.
RX PubMed=14654433; DOI=10.1016/s1567-1356(03)00166-1;
RA Verstrepen K.J., Derdelinckx G., Dufour J.P., Winderickx J.,
RA Pretorius I.S., Thevelein J.M., Delvaux F.R.;
RT "The Saccharomyces cerevisiae alcohol acetyl transferase gene ATF1 is a
RT target of the cAMP/PKA and FGM nutrient-signalling pathways.";
RL FEMS Yeast Res. 4:285-296(2003).
RN [12]
RP FUNCTION.
RX PubMed=12937998; DOI=10.1007/s10295-003-0070-0;
RA Horton C.E., Huang K.X., Bennett G.N., Rudolph F.B.;
RT "Heterologous expression of the Saccharomyces cerevisiae alcohol
RT acetyltransferase genes in Clostridium acetobutylicum and Escherichia coli
RT for the production of isoamyl acetate.";
RL J. Ind. Microbiol. Biotechnol. 30:427-432(2003).
RN [13]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [14]
RP SUBCELLULAR LOCATION.
RX PubMed=15042596; DOI=10.1002/yea.1100;
RA Verstrepen K.J., Van Laere S.D., Vercammen J., Derdelinckx G., Dufour J.P.,
RA Pretorius I.S., Winderickx J., Thevelein J.M., Delvaux F.R.;
RT "The Saccharomyces cerevisiae alcohol acetyl transferase Atf1p is localized
RT in lipid particles.";
RL Yeast 21:367-377(2004).
RN [15]
RP FUNCTION, AND BIOTECHNOLOGY.
RX PubMed=16845703; DOI=10.1002/yea.1382;
RA Lilly M., Bauer F.F., Lambrechts M.G., Swiegers J.H., Cozzolino D.,
RA Pretorius I.S.;
RT "The effect of increased yeast alcohol acetyltransferase and esterase
RT activity on the flavour profiles of wine and distillates.";
RL Yeast 23:641-659(2006).
RN [16]
RP FUNCTION, AND BIOTECHNOLOGY.
RX PubMed=18597084; DOI=10.1007/s00253-008-1573-4;
RA Etschmann M.M., Koetter P., Hauf J., Bluemke W., Entian K.D., Schrader J.;
RT "Production of the aroma chemicals 3-(methylthio)-1-propanol and 3-
RT (methylthio)-propylacetate with yeasts.";
RL Appl. Microbiol. Biotechnol. 80:579-587(2008).
RN [17]
RP FUNCTION.
RX PubMed=17891501; DOI=10.1007/s00449-007-0159-3;
RA Singh R., Vadlani P.V., Harrison M.L., Bennett G.N., San K.Y.;
RT "Aerobic production of isoamyl acetate by overexpression of the yeast
RT alcohol acetyl-transferases AFT1 and AFT2 in Escherichia coli and using
RT low-cost fermentation ingredients.";
RL Bioprocess Biosyst. Eng. 31:299-306(2008).
RN [18]
RP FUNCTION.
RX PubMed=25310977; DOI=10.1016/j.celrep.2014.09.009;
RA Christiaens J.F., Franco L.M., Cools T.L., De Meester L., Michiels J.,
RA Wenseleers T., Hassan B.A., Yaksi E., Verstrepen K.J.;
RT "The fungal aroma gene ATF1 promotes dispersal of yeast cells through
RT insect vectors.";
RL Cell Rep. 9:425-432(2014).
RN [19]
RP FUNCTION, AND BIOTECHNOLOGY.
RX PubMed=25306884; DOI=10.1007/s10295-014-1522-4;
RA Dong J., Xu H., Zhao L., Chen Y., Zhang C., Guo X., Hou X., Chen D.,
RA Zhang C., Xiao D.;
RT "Enhanced acetate ester production of Chinese liquor yeast by
RT overexpressing ATF1 through precise and seamless insertion of PGK1
RT promoter.";
RL J. Ind. Microbiol. Biotechnol. 41:1823-1828(2014).
RN [20]
RP BIOTECHNOLOGY.
RX PubMed=25281839; DOI=10.1016/j.ymben.2014.09.006;
RA Layton D.S., Trinh C.T.;
RT "Engineering modular ester fermentative pathways in Escherichia coli.";
RL Metab. Eng. 26:77-88(2014).
RN [21]
RP BIOTECHNOLOGY.
RX PubMed=24609358; DOI=10.1038/nchembio.1476;
RA Rodriguez G.M., Tashiro Y., Atsumi S.;
RT "Expanding ester biosynthesis in Escherichia coli.";
RL Nat. Chem. Biol. 10:259-265(2014).
RN [22]
RP SUBCELLULAR LOCATION, AND DOMAIN.
RX PubMed=25093817; DOI=10.1371/journal.pone.0104141;
RA Lin J.L., Wheeldon I.;
RT "Dual N- and C-terminal helices are required for endoplasmic reticulum and
RT lipid droplet association of alcohol acetyltransferases in Saccharomyces
RT cerevisiae.";
RL PLoS ONE 9:E104141-E104141(2014).
RN [23]
RP BIOTECHNOLOGY.
RX PubMed=26205521; DOI=10.1007/s00253-015-6809-5;
RA Guo D., Pan H., Li X.;
RT "Metabolic engineering of Escherichia coli for production of biodiesel from
RT fatty alcohols and acetyl-CoA.";
RL Appl. Microbiol. Biotechnol. 99:7805-7812(2015).
RN [24]
RP BIOTECHNOLOGY.
RX PubMed=25447641; DOI=10.1016/j.ymben.2014.10.004;
RA Tai Y.S., Xiong M., Zhang K.;
RT "Engineered biosynthesis of medium-chain esters in Escherichia coli.";
RL Metab. Eng. 27:20-28(2015).
RN [25]
RP BIOTECHNOLOGY.
RX PubMed=26801935; DOI=10.1007/s11745-016-4122-4;
RA Ding B.J., Lager I., Bansal S., Durrett T.P., Stymne S., Loefstedt C.;
RT "The yeast ATF1 acetyltransferase efficiently acetylates insect pheromone
RT alcohols: implications for the biological production of moth pheromones.";
RL Lipids 51:469-475(2016).
RN [26]
RP SUBCELLULAR LOCATION.
RX PubMed=28497697; DOI=10.1021/acssynbio.7b00041;
RA Lin J.L., Zhu J., Wheeldon I.;
RT "Synthetic Protein Scaffolds for Biosynthetic Pathway Colocalization on
RT Lipid Droplet Membranes.";
RL ACS Synth. Biol. 6:1534-1544(2017).
RN [27]
RP BIOTECHNOLOGY.
RX PubMed=28176138; DOI=10.1007/s10295-017-1907-2;
RA Li W., Wang J.H., Zhang C.Y., Ma H.X., Xiao D.G.;
RT "Regulation of Saccharomyces cerevisiae genetic engineering on the
RT production of acetate esters and higher alcohols during Chinese Baijiu
RT fermentation.";
RL J. Ind. Microbiol. Biotechnol. 44:949-960(2017).
RN [28]
RP FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, AND
RP MUTAGENESIS OF HIS-191.
RX PubMed=28160314; DOI=10.1002/yea.3229;
RA Nancolas B., Bull I.D., Stenner R., Dufour V., Curnow P.;
RT "Saccharomyces cerevisiae Atf1p is an alcohol acetyltransferase and a
RT thioesterase in vitro.";
RL Yeast 34:239-251(2017).
CC -!- FUNCTION: Major alcohol O-acetyltransferase that uses acetyl-CoA to
CC synthesize acetate esters from various alcohols, producing ethyl
CC acetate, isoamyl acetate, isobutyl acetate, butyl acetate, hexyl
CC acetate, heptyl acetate and octyl acetate (PubMed:7764365,
CC PubMed:9758847, PubMed:10653746, PubMed:12937998, PubMed:16845703,
CC PubMed:18597084, PubMed:17891501, PubMed:25306884, PubMed:28160314).
CC The alcohol acyltransferase activity is promiscuous with regard to
CC alcohol but relatively specific for acetyl-CoA since ATF1 does not use
CC any other acyl-CoAs (C3, C4, C5, C6, C8, C10, C12) (PubMed:28160314).
CC Acts also as an efficient thioesterase in vitro with specificity
CC towards medium-chain-length acyl-CoAs (PubMed:28160314). In natural
CC environments, the production of aromatic volatile metabolites promotes
CC dispersal through insect vectors (PubMed:25310977).
CC {ECO:0000269|PubMed:10653746, ECO:0000269|PubMed:12937998,
CC ECO:0000269|PubMed:16845703, ECO:0000269|PubMed:17891501,
CC ECO:0000269|PubMed:18597084, ECO:0000269|PubMed:25306884,
CC ECO:0000269|PubMed:25310977, ECO:0000269|PubMed:28160314,
CC ECO:0000269|PubMed:7764365, ECO:0000269|PubMed:9758847}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + an aliphatic alcohol = an acetyl ester + CoA;
CC Xref=Rhea:RHEA:17229, ChEBI:CHEBI:2571, ChEBI:CHEBI:47622,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.84;
CC Evidence={ECO:0000269|PubMed:28160314, ECO:0000269|PubMed:7764365};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a fatty acyl-CoA + H2O = a fatty acid + CoA + H(+);
CC Xref=Rhea:RHEA:16781, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:28868, ChEBI:CHEBI:57287, ChEBI:CHEBI:77636; EC=3.1.2.20;
CC Evidence={ECO:0000269|PubMed:28160314};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-methylbutanol + acetyl-CoA = 3-methylbutyl acetate + CoA;
CC Xref=Rhea:RHEA:65236, ChEBI:CHEBI:15837, ChEBI:CHEBI:31725,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288;
CC Evidence={ECO:0000269|PubMed:7764365};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:65237;
CC Evidence={ECO:0000269|PubMed:7764365};
CC -!- ACTIVITY REGULATION: Found to be inhibited by cadmium, copper, zinc and
CC mercurium divalent cations and sulfhydryl reagents (PubMed:7764365).
CC Inhibited by the addition of unsaturated fatty acids to the culture
CC (PubMed:7764365). {ECO:0000269|PubMed:7764365}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.19 mM for acetyl-CoA (for acetyltransferase activity)
CC {ECO:0000269|PubMed:7764365};
CC KM=29.8 mM for isoamyl alcohol (for acetyltransferase activity)
CC {ECO:0000269|PubMed:7764365};
CC KM=61 uM for acetyl-CoA (for thioesterase activity)
CC {ECO:0000269|PubMed:28160314};
CC KM=25 uM for butyryl-CoA (for thioesterase activity)
CC {ECO:0000269|PubMed:28160314};
CC KM=12 uM for hexanoyl-CoA (for thioesterase activity)
CC {ECO:0000269|PubMed:28160314};
CC KM=3 uM for octanoyl-CoA (for thioesterase activity)
CC {ECO:0000269|PubMed:28160314};
CC pH dependence:
CC Optimum pH is 8. {ECO:0000269|PubMed:7764365};
CC Temperature dependence:
CC Optimum temperature is 25 degrees Celsius.
CC {ECO:0000269|PubMed:7764365};
CC -!- SUBCELLULAR LOCATION: Lipid droplet {ECO:0000269|PubMed:15042596,
CC ECO:0000269|PubMed:25093817, ECO:0000269|PubMed:28497697}. Endoplasmic
CC reticulum membrane {ECO:0000269|PubMed:25093817}; Peripheral membrane
CC protein {ECO:0000269|PubMed:25093817}. Note=Traffics to lipid droplets
CC during the stationary phase (PubMed:25093817).
CC {ECO:0000269|PubMed:25093817}.
CC -!- INDUCTION: Expression is repressed both by aeration and by unsaturated
CC fatty acids (PubMed:9055409, PubMed:9675816). Also repressed by heat
CC and ethanol stress (PubMed:14654433). Rapid induction by glucose
CC depends on the cAMP/PKA signaling pathway (PubMed:14654433). Long-term
CC expression requires both carbon and nitrogen sources (PubMed:14654433).
CC The activation of transcription is dependent on RAP1, and the ROX1-
CC TUP1p-SSN6 hypoxic repressor complex is responsible for repression by
CC oxygen (PubMed:10487921). The putative ROX1-binding sequence in the
CC ATF1 promoter is 5'-CCTATTGTTTT-3' and the RAP1-binding sequence is 5'-
CC AACCCAACAAA-3' (PubMed:10487921). {ECO:0000269|PubMed:10487921,
CC ECO:0000269|PubMed:14654433, ECO:0000269|PubMed:9055409,
CC ECO:0000269|PubMed:9675816}.
CC -!- DOMAIN: Segments of the N- and C-terminal domains (residues 24-41 and
CC 508-525, respectively) are predicted to be amphipathic helices and are
CC essential for endoplasmic reticulum and lipid dropplet association
CC (PubMed:25093817). {ECO:0000269|PubMed:25093817}.
CC -!- DISRUPTION PHENOTYPE: Reduces levels of isoamyl acetate production
CC during fermentation to about 16% of those produced by the wild-type
CC strain and causes a 40% reduction of ethyl acetate formation
CC (PubMed:12957907). {ECO:0000269|PubMed:12957907}.
CC -!- BIOTECHNOLOGY: Understanding the biochemistry of the volatile esters is
CC of considerable importance in industrial agriculture, winemaking and
CC brewing. ATF1 could profoundly affect the flavor profiles of wines and
CC distillates deficient in aroma, thereby paving the way for the
CC production of products maintaining a fruitier character for longer
CC periods after bottling (PubMed:10653746, PubMed:16845703,
CC PubMed:18597084, PubMed:25306884, PubMed:28176138). The most important
CC acetate esters from the perspective of fermented foods and beverages
CC are probably ethyl acetate (fruity, solvent aroma), isobutyl acetate
CC (sweet, fruit), isoamyl acetate (banana) and 2-phenylethyl acetate
CC (rose). In addition, the enzymes responsible for ester synthesis are
CC targets for the metabolic engineering of cellular factories intended to
CC produce insect pheromones for use in pest control, but also for the
CC production of fragrances, industrial solvents, fine chemicals,
CC pharmaceuticals and renewable biofuels (PubMed:25281839,
CC PubMed:24609358, PubMed:26205521, PubMed:26801935).
CC {ECO:0000269|PubMed:10653746, ECO:0000269|PubMed:16845703,
CC ECO:0000269|PubMed:18597084, ECO:0000269|PubMed:24609358,
CC ECO:0000269|PubMed:25281839, ECO:0000269|PubMed:25306884,
CC ECO:0000269|PubMed:25447641, ECO:0000269|PubMed:26205521,
CC ECO:0000269|PubMed:26801935, ECO:0000269|PubMed:28176138}.
CC -!- MISCELLANEOUS: Present with 1990 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the ATF1 alcohol acetyltransferase family.
CC {ECO:0000305}.
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DR EMBL; D26554; BAA05552.1; -; Genomic_DNA.
DR EMBL; Z75285; CAA99708.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA11136.1; -; Genomic_DNA.
DR PIR; S67289; S67289.
DR RefSeq; NP_015022.3; NM_001183797.3.
DR AlphaFoldDB; P40353; -.
DR BioGRID; 34759; 41.
DR DIP; DIP-5494N; -.
DR IntAct; P40353; 2.
DR STRING; 4932.YOR377W; -.
DR MaxQB; P40353; -.
DR PaxDb; P40353; -.
DR PRIDE; P40353; -.
DR EnsemblFungi; YOR377W_mRNA; YOR377W; YOR377W.
DR GeneID; 854559; -.
DR KEGG; sce:YOR377W; -.
DR SGD; S000005904; ATF1.
DR VEuPathDB; FungiDB:YOR377W; -.
DR eggNOG; ENOG502QTAU; Eukaryota.
DR GeneTree; ENSGT00940000176620; -.
DR HOGENOM; CLU_043707_0_0_1; -.
DR InParanoid; P40353; -.
DR OMA; FAEINDF; -.
DR BioCyc; MetaCyc:YOR377W-MON; -.
DR BioCyc; YEAST:YOR377W-MON; -.
DR BRENDA; 2.3.1.84; 984.
DR SABIO-RK; P40353; -.
DR PRO; PR:P40353; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; P40353; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005811; C:lipid droplet; IDA:SGD.
DR GO; GO:0047617; F:acyl-CoA hydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0004026; F:alcohol O-acetyltransferase activity; IDA:SGD.
DR GO; GO:0008080; F:N-acetyltransferase activity; IBA:GO_Central.
DR GO; GO:1901089; P:acetate ester metabolic process involved in fermentation; IMP:SGD.
DR InterPro; IPR010828; Atf2/Sli1-like.
DR Pfam; PF07247; AATase; 1.
PE 1: Evidence at protein level;
KW Acyltransferase; Direct protein sequencing; Endoplasmic reticulum;
KW Hydrolase; Lipid droplet; Membrane; Reference proteome; Transferase.
FT CHAIN 1..525
FT /note="Alcohol O-acetyltransferase 1"
FT /id="PRO_0000064720"
FT REGION 24..41
FT /note="Membrane association"
FT /evidence="ECO:0000269|PubMed:25093817"
FT REGION 508..525
FT /note="Membrane association"
FT /evidence="ECO:0000269|PubMed:25093817"
FT ACT_SITE 191
FT /note="Charge relay system"
FT /evidence="ECO:0000305|PubMed:28160314"
FT ACT_SITE 195
FT /note="Charge relay system"
FT /evidence="ECO:0000305|PubMed:28160314"
FT MUTAGEN 191
FT /note="H->A: Only moderately reduces the thioesterase
FT activity."
FT /evidence="ECO:0000269|PubMed:28160314"
FT CONFLICT 84
FT /note="T -> I (in Ref. 1; BAA05552)"
FT /evidence="ECO:0000305"
FT CONFLICT 391
FT /note="N -> K (in Ref. 1; BAA05552)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 525 AA; 61036 MW; 79D0733CDDC5C629 CRC64;
MNEIDEKNQA PVQQECLKEM IQNGHARRMG SVEDLYVALN RQNLYRNFCT YGELSDYCTR
DQLTLALREI CLKNPTLLHI VLPTRWPNHE NYYRSSEYYS RPHPVHDYIS VLQELKLSGV
VLNEQPEYSA VMKQILEEFK NSKGSYTAKI FKLTTTLTIP YFGPTGPSWR LICLPEEHTE
KWKKFIFVSN HCMSDGRSSI HFFHDLRDEL NNIKTPPKKL DYIFKYEEDY QLLRKLPEPI
EKVIDFRPPY LFIPKSLLSG FIYNHLRFSS KGVCMRMDDV EKTDDVVTEI INISPTEFQA
IKANIKSNIQ GKCTITPFLH VCWFVSLHKW GKFFKPLNFE WLTDIFIPAD CRSQLPDDDE
MRQMYRYGAN VGFIDFTPWI SEFDMNDNKE NFWPLIEHYH EVISEALRNK KHLHGLGFNI
QGFVQKYVNI DKVMCDRAIG KRRGGTLLSN VGLFNQLEEP DAKYSICDLA FGQFQGSWHQ
AFSLGVCSTN VKGMNIVVAS TKNVVGSQES LEELCSIYKA LLLGP