PPK28_DROME
ID PPK28_DROME Reviewed; 606 AA.
AC Q86LG1; Q86LG0; Q9VXA6;
DT 28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT 11-JUN-2014, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Pickpocket protein 28 {ECO:0000303|PubMed:12571352};
DE Short=PPK28 {ECO:0000303|PubMed:12571352, ECO:0000312|EMBL:AAO47378.1};
GN Name=ppk28; ORFNames=CG4805;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAO47378.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12571352; DOI=10.1073/pnas.252785099;
RA Liu L., Johnson W.A., Welsh M.J.;
RT "Drosophila DEG/ENaC pickpocket genes are expressed in the tracheal system,
RT where they may be involved in liquid clearance.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:2128-2133(2003).
RN [2] {ECO:0000312|EMBL:AAF48671.3}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000305, ECO:0000312|EMBL:ADE06680.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP IDENTIFICATION.
RX PubMed=12848938; DOI=10.1016/s0896-6273(03)00394-5;
RA Liu L., Leonard A.S., Motto D.G., Feller M.A., Price M.P., Johnson W.A.,
RA Welsh M.J.;
RT "Contribution of Drosophila DEG/ENaC genes to salt taste.";
RL Neuron 39:133-146(2003).
RN [6] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20445050; DOI=10.1523/jneurosci.0627-10.2010;
RA Chen Z., Wang Q., Wang Z.;
RT "The amiloride-sensitive epithelial Na+ channel PPK28 is essential for
RT drosophila gustatory water reception.";
RL J. Neurosci. 30:6247-6252(2010).
RN [7] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=20364123; DOI=10.1038/nature09011;
RA Cameron P., Hiroi M., Ngai J., Scott K.;
RT "The molecular basis for water taste in Drosophila.";
RL Nature 465:91-95(2010).
CC -!- FUNCTION: Osmosensitive ion channel that mediates the cellular and
CC behavioral response to water. Plays an essential role in gustatory
CC water reception. Part of a complex that plays a role in tracheal liquid
CC clearance. Probable role in sodium transport.
CC {ECO:0000269|PubMed:12571352, ECO:0000269|PubMed:20364123,
CC ECO:0000269|PubMed:20445050, ECO:0000303|PubMed:12571352}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:20364123};
CC Multi-pass membrane protein {ECO:0000269|PubMed:20364123}.
CC -!- TISSUE SPECIFICITY: Expressed in water-sensing neurons in taste
CC bristles on the proboscis but not in carbonation-sensing taste peg
CC neurons (at protein level). Expressed in the tracheal system.
CC {ECO:0000269|PubMed:12571352, ECO:0000269|PubMed:20364123}.
CC -!- DISRUPTION PHENOTYPE: Flies do not exhibit any general drinking
CC defects, but do exhibit markedly reduced water-elicited proboscis
CC extension reflex responses. Abolishes water-elicited spiking activity
CC of water gustatory neurons. {ECO:0000269|PubMed:20364123,
CC ECO:0000269|PubMed:20445050}.
CC -!- SIMILARITY: Belongs to the amiloride-sensitive sodium channel (TC
CC 1.A.6) family. {ECO:0000255}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAO47378.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact.; Evidence={ECO:0000305};
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DR EMBL; AY226552; AAO47378.1; ALT_SEQ; mRNA.
DR EMBL; AY226553; AAO47379.1; -; mRNA.
DR EMBL; AE014298; AAF48671.3; -; Genomic_DNA.
DR EMBL; BT122074; ADE06680.1; -; mRNA.
DR RefSeq; NP_573169.2; NM_132941.3.
DR AlphaFoldDB; Q86LG1; -.
DR BioGRID; 59002; 3.
DR IntAct; Q86LG1; 1.
DR STRING; 7227.FBpp0074109; -.
DR PaxDb; Q86LG1; -.
DR DNASU; 32671; -.
DR EnsemblMetazoa; FBtr0074335; FBpp0074109; FBgn0030795.
DR GeneID; 32671; -.
DR KEGG; dme:Dmel_CG4805; -.
DR UCSC; CG4805-RA; d. melanogaster.
DR UCSC; CG4805-RB; d. melanogaster.
DR CTD; 32671; -.
DR FlyBase; FBgn0030795; ppk28.
DR VEuPathDB; VectorBase:FBgn0030795; -.
DR eggNOG; KOG4294; Eukaryota.
DR GeneTree; ENSGT00940000169532; -.
DR HOGENOM; CLU_024950_1_0_1; -.
DR InParanoid; Q86LG1; -.
DR OMA; RVCGPND; -.
DR OrthoDB; 686369at2759; -.
DR PhylomeDB; Q86LG1; -.
DR Reactome; R-DME-2672351; Stimuli-sensing channels.
DR BioGRID-ORCS; 32671; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 32671; -.
DR PRO; PR:Q86LG1; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0030795; Expressed in gustatory receptor neuron (Drosophila) and 9 other tissues.
DR Genevisible; Q86LG1; DM.
DR GO; GO:0016021; C:integral component of membrane; ISS:FlyBase.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0015280; F:ligand-gated sodium channel activity; IBA:GO_Central.
DR GO; GO:0005272; F:sodium channel activity; ISS:FlyBase.
DR GO; GO:0071462; P:cellular response to water stimulus; IMP:FlyBase.
DR GO; GO:0042756; P:drinking behavior; IMP:FlyBase.
DR GO; GO:0035002; P:liquid clearance, open tracheal system; IMP:UniProtKB.
DR GO; GO:0009415; P:response to water; IDA:FlyBase.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006814; P:sodium ion transport; ISS:FlyBase.
DR InterPro; IPR001873; ENaC.
DR PANTHER; PTHR11690; PTHR11690; 1.
DR Pfam; PF00858; ASC; 1.
DR PRINTS; PR01078; AMINACHANNEL.
PE 1: Evidence at protein level;
KW Cell membrane; Ion channel; Ion transport; Membrane; Reference proteome;
KW Sodium; Sodium channel; Sodium transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..606
FT /note="Pickpocket protein 28"
FT /id="PRO_0000420433"
FT TRANSMEM 66..86
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 490..510
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 606 AA; 69225 MW; A7B7BA093756D74A CRC64;
MRTLTESRRR QSGSSGCKKD SESDDDENTI CSRAAIKRSV VYYLKNSTLH GLKYIAEESI
TIPERIFFGL AFVLVVILSV FFISNVYVKW SASPIIISTS AKQKLTSNMP FPAITICNLN
QALLSKVDRI GRTSTNFSLL MGLCDQGGDT TISYIGTWKY FKAILVDVAQ PCEKMLLYCS
FGSREEDCSW LFTSILTDDG LCCNFNALHP SYLIRNYSDD VRLETAHPNT RYELIDWTPE
KGYARNLPEF YFPRTSGGTG IRMGLTVVLN ASIAEYYCTK SMSVGFKVLV HNPAELPKVS
NYGFVVTAGR EARIPIEPVY EDALPTIRSI KKSVRRCLFS DENDLAYYRT YSRKNCELEC
EAKLLLRECS CVLYYLPRID PLARVCGPND NQCTDRVQTE IESSLTNLSC ENCWPGCFEL
TYRATLSTAS IVSDPRFQAG ENLPEYIFHG PYSNASEISI LHFYYMTNIF RSTTKSEMFG
FTEFLSNTGG LLGLFMGFSI FSVIEIFFYI TVRPYCASRT LRQRHKRRLE QLSWLTPIRM
PVRRALRRNR GGLLRNPPPP AYSDLQKFRG KLDKPETCKR KLWRTLQVRP VVDRADEELP
TYPYLD
