ID   PPK32_SCHPO             Reviewed;         749 AA.
AC   Q9P7X5;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Protein kinase domain-containing protein ppk32;
GN   Name=ppk32; ORFNames=SPBP23A10.10;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
RN   [3]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-632, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY.
RX   PubMed=18257517; DOI=10.1021/pr7006335;
RA   Wilson-Grady J.T., Villen J., Gygi S.P.;
RT   "Phosphoproteome analysis of fission yeast.";
RL   J. Proteome Res. 7:1088-1097(2008).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}.
CC   -!- DOMAIN: The protein kinase domain is predicted to be catalytically
CC       inactive.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CU329671; CAB66438.1; -; Genomic_DNA.
DR   PIR; T50397; T50397.
DR   RefSeq; NP_595822.1; NM_001021726.2.
DR   AlphaFoldDB; Q9P7X5; -.
DR   SMR; Q9P7X5; -.
DR   BioGRID; 277839; 46.
DR   STRING; 4896.SPBP23A10.10.1; -.
DR   iPTMnet; Q9P7X5; -.
DR   MaxQB; Q9P7X5; -.
DR   PaxDb; Q9P7X5; -.
DR   PRIDE; Q9P7X5; -.
DR   EnsemblFungi; SPBP23A10.10.1; SPBP23A10.10.1:pep; SPBP23A10.10.
DR   GeneID; 2541327; -.
DR   KEGG; spo:SPBP23A10.10; -.
DR   PomBase; SPBP23A10.10; ppk32.
DR   VEuPathDB; FungiDB:SPBP23A10.10; -.
DR   eggNOG; KOG2137; Eukaryota.
DR   HOGENOM; CLU_008724_1_1_1; -.
DR   InParanoid; Q9P7X5; -.
DR   OMA; PKMTQPN; -.
DR   PhylomeDB; Q9P7X5; -.
DR   PRO; PR:Q9P7X5; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0005737; C:cytoplasm; IDA:PomBase.
DR   GO; GO:0005524; F:ATP binding; ISM:PomBase.
DR   GO; GO:0004674; F:protein serine/threonine kinase activity; TAS:PomBase.
DR   GO; GO:1904262; P:negative regulation of TORC1 signaling; IMP:PomBase.
DR   GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR   Gene3D; 1.25.10.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR000719; Prot_kinase_dom.
DR   Pfam; PF00069; Pkinase; 1.
DR   SMART; SM00220; S_TKc; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
DR   PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Phosphoprotein; Reference proteome.
FT   CHAIN           1..749
FT                   /note="Protein kinase domain-containing protein ppk32"
FT                   /id="PRO_0000256832"
FT   DOMAIN          21..317
FT                   /note="Protein kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   REGION          598..677
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          706..749
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        714..749
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         632
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18257517"
SQ   SEQUENCE   749 AA;  82824 MW;  3894DAA925EE3602 CRC64;
     MLANALRLVS NSKKIKYDYD IQKENSVQVG PWTVYSASKK GTNEEVSVFT FDKKNLSTLL
     KRGSIDSNLK TNYVLELLRK DVSSLSRLRH PSLLQVVEPL EESKSSMSFV TRRIQSMLQD
     FIKSSNGGFS NYGSSANGKS SGNALEEVEI QKGLLQIIDG LVFLHGSAKV IHYNIRPSSV
     VVDAKGDWKL CGFSFSQSVE SARYEFNDYD FGIPSSLQQS MDFLAPEYIT HEIAGPESDV
     FSFGCLIYSI FNKNQSIINA NNHLLSYEKE ITSLNSPTFI ESKNLPSENL KSLLKETLAV
     DPKQRASMFE LERSPYFTGS AIAALRFLES FPEKLPSEKV SFMESLSKNL TTFPYRIQSQ
     KILPTLLDHL NDQKLVPSLL PCIFEISKGL DSSIFSSKVF TAIFPIISAA NSYPERVPLC
     IFQYMDCLKS KLPSGEFLSK IVPFIYGCFE NSSLNVQTTS IQILGTLLDI IDVTTVKSSI
     CPKLYHSFSV TNQLDVKVAI LDTFNVFINQ KFLDSFAIVD KLLPVLEKVK TREPTVVMGM
     VTVYISAGAI IPEETVHEQV IPRLWILSVS PSLSLEQYNK CMREIRSLSD AVQKSHAKKL
     QSKPSSVVPN RITTDPFSSQ TKEATSKPSS ISPNKATTNI FTSQASLSSQ GVARETSSAS
     SYRSYSQRAS TPAVTAKSSF HYATPTSGLS NFNSVTPSSS ASLYPPLIPS EARTPSVQPA
     NRRVTTPVVN QNTVTSDSSN DLGGWKSLL