AA2BR_HUMAN
ID AA2BR_HUMAN Reviewed; 332 AA.
AC P29275;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-1992, sequence version 1.
DT 03-AUG-2022, entry version 191.
DE RecName: Full=Adenosine receptor A2b;
GN Name=ADORA2B;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=1325798; DOI=10.1016/s0006-291x(05)81462-7;
RA Pierce K.D., Furlong T.J., Selbie L.A., Shine J.;
RT "Molecular cloning and expression of an adenosine A2b receptor from human
RT brain.";
RL Biochem. Biophys. Res. Commun. 187:86-93(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=7558011; DOI=10.1006/geno.1995.1061;
RA Jacobson M.A., Johnson R.G., Luneau C.J., Salvatore C.A.;
RT "Cloning and chromosomal localization of the human A2b adenosine receptor
RT gene (ADORA2B) and its pseudogene.";
RL Genomics 27:374-376(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RA Puhl H.L. III, Ikeda S.R., Aronstam R.S.;
RT "cDNA clones of human proteins involved in signal transduction sequenced by
RT the Guthrie cDNA resource center (www.cdna.org).";
RL Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Receptor for adenosine. The activity of this receptor is
CC mediated by G proteins which activate adenylyl cyclase.
CC -!- INTERACTION:
CC P29275; P29274: ADORA2A; NbExp=5; IntAct=EBI-3904751, EBI-2902702;
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; M97759; AAA51598.1; -; mRNA.
DR EMBL; X68487; CAA48505.1; -; mRNA.
DR EMBL; AY136748; AAN01274.1; -; mRNA.
DR EMBL; BC025722; AAH25722.1; -; mRNA.
DR CCDS; CCDS11173.1; -.
DR PIR; JC1229; JC1229.
DR RefSeq; NP_000667.1; NM_000676.2.
DR AlphaFoldDB; P29275; -.
DR SMR; P29275; -.
DR BioGRID; 106648; 46.
DR IntAct; P29275; 3.
DR STRING; 9606.ENSP00000304501; -.
DR BindingDB; P29275; -.
DR ChEMBL; CHEMBL255; -.
DR DrugBank; DB00640; Adenosine.
DR DrugBank; DB00201; Caffeine.
DR DrugBank; DB05936; CVT-6883.
DR DrugBank; DB04932; Defibrotide.
DR DrugBank; DB00824; Enprofylline.
DR DrugBank; DB00277; Theophylline.
DR DrugCentral; P29275; -.
DR GuidetoPHARMACOLOGY; 20; -.
DR GlyGen; P29275; 2 sites.
DR iPTMnet; P29275; -.
DR PhosphoSitePlus; P29275; -.
DR BioMuta; ADORA2B; -.
DR DMDM; 112938; -.
DR MassIVE; P29275; -.
DR PaxDb; P29275; -.
DR PeptideAtlas; P29275; -.
DR PRIDE; P29275; -.
DR Antibodypedia; 25203; 315 antibodies from 37 providers.
DR DNASU; 136; -.
DR Ensembl; ENST00000304222.3; ENSP00000304501.2; ENSG00000170425.4.
DR GeneID; 136; -.
DR KEGG; hsa:136; -.
DR MANE-Select; ENST00000304222.3; ENSP00000304501.2; NM_000676.4; NP_000667.1.
DR UCSC; uc002gpd.2; human.
DR CTD; 136; -.
DR DisGeNET; 136; -.
DR GeneCards; ADORA2B; -.
DR HGNC; HGNC:264; ADORA2B.
DR HPA; ENSG00000170425; Tissue enhanced (brain, urinary bladder).
DR MIM; 600446; gene.
DR neXtProt; NX_P29275; -.
DR OpenTargets; ENSG00000170425; -.
DR PharmGKB; PA24585; -.
DR VEuPathDB; HostDB:ENSG00000170425; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01030000234555; -.
DR HOGENOM; CLU_009579_11_5_1; -.
DR InParanoid; P29275; -.
DR OMA; PVKCLFE; -.
DR OrthoDB; 550297at2759; -.
DR PhylomeDB; P29275; -.
DR TreeFam; TF325296; -.
DR PathwayCommons; P29275; -.
DR Reactome; R-HSA-417973; Adenosine P1 receptors.
DR Reactome; R-HSA-418555; G alpha (s) signalling events.
DR Reactome; R-HSA-5683826; Surfactant metabolism.
DR Reactome; R-HSA-9660821; ADORA2B mediated anti-inflammatory cytokines production.
DR SignaLink; P29275; -.
DR SIGNOR; P29275; -.
DR BioGRID-ORCS; 136; 5 hits in 1076 CRISPR screens.
DR ChiTaRS; ADORA2B; human.
DR GeneWiki; Adenosine_A2B_receptor; -.
DR GenomeRNAi; 136; -.
DR Pharos; P29275; Tclin.
DR PRO; PR:P29275; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; P29275; protein.
DR Bgee; ENSG00000170425; Expressed in mucosa of transverse colon and 131 other tissues.
DR Genevisible; P29275; HS.
DR GO; GO:0098978; C:glutamatergic synapse; IEA:Ensembl.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0098685; C:Schaffer collateral - CA1 synapse; IEA:Ensembl.
DR GO; GO:0001609; F:G protein-coupled adenosine receptor activity; IEA:InterPro.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0007190; P:activation of adenylate cyclase activity; TAS:ProtInc.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0031668; P:cellular response to extracellular stimulus; IEA:Ensembl.
DR GO; GO:0019934; P:cGMP-mediated signaling; IEA:Ensembl.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0043303; P:mast cell degranulation; IEA:Ensembl.
DR GO; GO:0010753; P:positive regulation of cGMP-mediated signaling; IEA:Ensembl.
DR GO; GO:0032722; P:positive regulation of chemokine production; IEA:Ensembl.
DR GO; GO:0002882; P:positive regulation of chronic inflammatory response to non-antigenic stimulus; IEA:Ensembl.
DR GO; GO:0031284; P:positive regulation of guanylate cyclase activity; IEA:Ensembl.
DR GO; GO:0032755; P:positive regulation of interleukin-6 production; IEA:Ensembl.
DR GO; GO:0043306; P:positive regulation of mast cell degranulation; IEA:Ensembl.
DR GO; GO:0010575; P:positive regulation of vascular endothelial growth factor production; IEA:Ensembl.
DR GO; GO:2000300; P:regulation of synaptic vesicle exocytosis; IEA:Ensembl.
DR GO; GO:0060087; P:relaxation of vascular associated smooth muscle; IEA:Ensembl.
DR GO; GO:0042311; P:vasodilation; IBA:GO_Central.
DR InterPro; IPR001435; Adeno_A2B_rcpt.
DR InterPro; IPR001634; Adenosn_rcpt.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00554; ADENOSINA2BR.
DR PRINTS; PR00424; ADENOSINER.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Lipoprotein; Membrane; Palmitate; Receptor; Reference proteome; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..332
FT /note="Adenosine receptor A2b"
FT /id="PRO_0000069003"
FT TOPO_DOM 1..8
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 9..33
FT /note="Helical; Name=1"
FT /evidence="ECO:0000250"
FT TOPO_DOM 34..43
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 44..67
FT /note="Helical; Name=2"
FT /evidence="ECO:0000250"
FT TOPO_DOM 68..78
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 79..101
FT /note="Helical; Name=3"
FT /evidence="ECO:0000250"
FT TOPO_DOM 102..121
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 122..144
FT /note="Helical; Name=4"
FT /evidence="ECO:0000250"
FT TOPO_DOM 145..178
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 179..203
FT /note="Helical; Name=5"
FT /evidence="ECO:0000250"
FT TOPO_DOM 204..235
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 236..259
FT /note="Helical; Name=6"
FT /evidence="ECO:0000250"
FT TOPO_DOM 260..267
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 268..291
FT /note="Helical; Name=7"
FT /evidence="ECO:0000250"
FT TOPO_DOM 292..332
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT BINDING 174
FT /ligand="adenosine"
FT /ligand_id="ChEBI:CHEBI:16335"
FT /ligand_note="agonist"
FT /evidence="ECO:0000250|UniProtKB:P29274"
FT BINDING 254
FT /ligand="adenosine"
FT /ligand_id="ChEBI:CHEBI:16335"
FT /ligand_note="agonist"
FT /evidence="ECO:0000250|UniProtKB:P29274"
FT BINDING 279
FT /ligand="adenosine"
FT /ligand_id="ChEBI:CHEBI:16335"
FT /ligand_note="agonist"
FT /evidence="ECO:0000250|UniProtKB:P29274"
FT BINDING 280
FT /ligand="adenosine"
FT /ligand_id="ChEBI:CHEBI:16335"
FT /ligand_note="agonist"
FT /evidence="ECO:0000250|UniProtKB:P29274"
FT LIPID 311
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT CARBOHYD 153
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 163
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 78..171
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 332 AA; 36333 MW; 38AD088E2439C7FA CRC64;
MLLETQDALY VALELVIAAL SVAGNVLVCA AVGTANTLQT PTNYFLVSLA AADVAVGLFA
IPFAITISLG FCTDFYGCLF LACFVLVLTQ SSIFSLLAVA VDRYLAICVP LRYKSLVTGT
RARGVIAVLW VLAFGIGLTP FLGWNSKDSA TNNCTEPWDG TTNESCCLVK CLFENVVPMS
YMVYFNFFGC VLPPLLIMLV IYIKIFLVAC RQLQRTELMD HSRTTLQREI HAAKSLAMIV
GIFALCWLPV HAVNCVTLFQ PAQGKNKPKW AMNMAILLSH ANSVVNPIVY AYRNRDFRYT
FHKIISRYLL CQADVKSGNG QAGVQPALGV GL
