ATF3_DROME
ID ATF3_DROME Reviewed; 613 AA.
AC Q9XZS8; Q8MRE5;
DT 11-MAY-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=Activating transcription factor 3 {ECO:0000312|FlyBase:FBgn0028550};
GN Name=Atf3 {ECO:0000312|FlyBase:FBgn0028550};
GN Synonyms=A3-3 {ECO:0000312|FlyBase:FBgn0028550};
GN ORFNames=CG11405 {ECO:0000312|FlyBase:FBgn0028550};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:CAB65887.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R {ECO:0000312|EMBL:CAB65887.1};
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [4] {ECO:0000312|EMBL:AAM51955.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM51955.1};
RC TISSUE=Head {ECO:0000312|EMBL:AAM51955.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5] {ECO:0000305}
RP FUNCTION, INTERACTION WITH JRA, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=20023169; DOI=10.1242/dev.037861;
RA Sekyrova P., Bohmann D., Jindra M., Uhlirova M.;
RT "Interaction between Drosophila bZIP proteins Atf3 and Jun prevents
RT replacement of epithelial cells during metamorphosis.";
RL Development 137:141-150(2010).
RN [6] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=22851689; DOI=10.1128/mcb.00429-12;
RA Rynes J., Donohoe C.D., Frommolt P., Brodesser S., Jindra M., Uhlirova M.;
RT "Activating transcription factor 3 regulates immune and metabolic
RT homeostasis.";
RL Mol. Cell. Biol. 32:3949-3962(2012).
RN [7] {ECO:0000305}
RP FUNCTION.
RX PubMed=26848073; DOI=10.1038/srep20109;
RA Bhat S., Jones W.D.;
RT "An accelerated miRNA-based screen implicates Atf-3 in Drosophila odorant
RT receptor expression.";
RL Sci. Rep. 6:20109-20109(2016).
CC -!- FUNCTION: Transcription factor which binds to the cAMP response element
CC (CRE) (PubMed:20023169). Regulates metabolic and innate immune
CC homeostasis, possibly by controlling appropriate expression of genes
CC involved in peritrophic matrix composition and ensuring the normal
CC digestive and immune function of the gut (PubMed:22851689). Required
CC for the expression of odorant receptors Or43b and Or47b
CC (PubMed:26848073). {ECO:0000269|PubMed:20023169,
CC ECO:0000269|PubMed:22851689, ECO:0000269|PubMed:26848073}.
CC -!- SUBUNIT: Interacts with Jra/jun; the interaction enhances the DNA-
CC binding activity of Atf3. {ECO:0000269|PubMed:20023169}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22851689}.
CC -!- TISSUE SPECIFICITY: Moderate expression in some regions of the larval
CC nervous system, the ring gland and imaginal disks. High expression in
CC larval gut, excretory malpighian tubules, salivary glands, and, to a
CC lesser extent, the fat body where levels are approximately 2.5-fold
CC less than the gut. {ECO:0000269|PubMed:22851689}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryo and larva with a sharp decline
CC by the late third instar larval stage. Expression peaks 6 hours after
CC puparium formation, drops and remains low until the second day of pupal
CC development and then grows steadily during adult morphogenesis.
CC {ECO:0000269|PubMed:20023169}.
CC -!- DISRUPTION PHENOTYPE: Mutants die soon after hatching and during all
CC three larval stages with only 2% reaching the third instar larval stage
CC and these die before metamorphosis (PubMed:20023169). Altered
CC expression levels of a number of genes in third instar larvae and
CC chronic inflammation and starvation responses in the larval gut
CC epithelium with a predominance of extremely large lipid droplets and
CC obesity but not hyperglycemia (PubMed:22851689).
CC {ECO:0000269|PubMed:20023169, ECO:0000269|PubMed:22851689}.
CC -!- SIMILARITY: Belongs to the bZIP family. ATF subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM51955.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE014298; AAF45599.1; -; Genomic_DNA.
DR EMBL; AE014298; AHN59240.1; -; Genomic_DNA.
DR EMBL; AL121806; CAB65887.1; -; Genomic_DNA.
DR EMBL; AY121628; AAM51955.1; ALT_TERM; mRNA.
DR RefSeq; NP_001284769.1; NM_001297840.1.
DR RefSeq; NP_620473.1; NM_138988.4.
DR AlphaFoldDB; Q9XZS8; -.
DR SMR; Q9XZS8; -.
DR IntAct; Q9XZS8; 3.
DR STRING; 7227.FBpp0070234; -.
DR PaxDb; Q9XZS8; -.
DR PRIDE; Q9XZS8; -.
DR EnsemblMetazoa; FBtr0070244; FBpp0070234; FBgn0028550.
DR EnsemblMetazoa; FBtr0345507; FBpp0311616; FBgn0028550.
DR GeneID; 43867; -.
DR KEGG; dme:Dmel_CG11405; -.
DR UCSC; CG11405-RA; d. melanogaster.
DR CTD; 467; -.
DR FlyBase; FBgn0028550; Atf3.
DR VEuPathDB; VectorBase:FBgn0028550; -.
DR eggNOG; KOG1414; Eukaryota.
DR GeneTree; ENSGT00940000174029; -.
DR HOGENOM; CLU_444301_0_0_1; -.
DR InParanoid; Q9XZS8; -.
DR OMA; MATFKFG; -.
DR OrthoDB; 828721at2759; -.
DR PhylomeDB; Q9XZS8; -.
DR SignaLink; Q9XZS8; -.
DR BioGRID-ORCS; 43867; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 43867; -.
DR PRO; PR:Q9XZS8; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0028550; Expressed in mouthpart and 20 other tissues.
DR ExpressionAtlas; Q9XZS8; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0006955; P:immune response; IMP:FlyBase.
DR GO; GO:0055088; P:lipid homeostasis; IMP:FlyBase.
DR GO; GO:0007399; P:nervous system development; IMP:FlyBase.
DR GO; GO:0010883; P:regulation of lipid storage; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR000837; AP-1.
DR InterPro; IPR004827; bZIP.
DR InterPro; IPR046347; bZIP_sf.
DR PANTHER; PTHR23351; PTHR23351; 1.
DR Pfam; PF00170; bZIP_1; 1.
DR PRINTS; PR00042; LEUZIPPRFOS.
DR SMART; SM00338; BRLZ; 1.
DR SUPFAM; SSF57959; SSF57959; 1.
DR PROSITE; PS50217; BZIP; 1.
DR PROSITE; PS00036; BZIP_BASIC; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..613
FT /note="Activating transcription factor 3"
FT /id="PRO_0000436179"
FT DOMAIN 205..268
FT /note="bZIP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 77..115
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 133..218
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 207..229
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 233..261
FT /note="Leucine-zipper"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 337..446
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 80..115
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 133..151
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 157..177
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 190..212
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 350..391
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 398..446
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 613 AA; 65161 MW; C321092F06C7BD7C CRC64;
MFNSNIPASS LLSIDSGGLL MGGHTPKTPE ILNSLIAMTN PLENFSYSSA AAAAAAVSVA
SSSASCSAAS TPVVTVRHFN GHPNGQSHSQ DSSHSSCSGS PLDSPAGTAT TPSVQQTCSR
LIKEGLKLSI QSKRKLSTCD SSSGSEQPHS KYSRRSSNHN GHSGSSNNYS GSMSNANDLD
DDCEESSDDD SETKSQPKGL TPEDEDRRRR RRERNKIAAT KCRMKKRERT QNLIKESEVL
DTQNVELKNQ VRQLETERQK LVDMLKSHGC QRAGGCQLPS QLLQSPAQKY LSELELETVS
IDGPNSGNNN QRLQSIPSMA TFKYGSKTAA AMAQQLPNGY CKPSPSAQEF EHAGYQQQQQ
QQQQQQPQSL NPAGNNVIDQ QHANPSPSLL SDYVPNCDGL TGSASNHPSH NNNNNNNNSS
GASSNTSNNN SNISSHSSNA TSSTTPTATS SAIEFVKNEL VDSQSPYTTA LSAERFLFEP
SDGFPDIKHA CVLPSPCGIN GLNMASVVNT NGSTGNNNNS HHNNNNNNNN HLMDFHQGLQ
HGNIGTGVGV GVGVGVGVGV GVGVMTPYDD EQLLLLKNGC FATDLLSQLV EEGGEYVDLD
SATAFMNNGS CLA
