ID   PPLA_CHICK              Reviewed;          52 AA.
AC   P26677;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Cardiac phospholamban;
DE            Short=PLB;
GN   Name=PLN;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND TISSUE SPECIFICITY.
RX   PubMed=1825996; DOI=10.1016/s0021-9258(19)67604-0;
RA   Toyofuku T., Zak R.;
RT   "Characterization of cDNA and genomic sequences encoding a chicken
RT   phospholamban.";
RL   J. Biol. Chem. 266:5375-5383(1991).
CC   -!- FUNCTION: Reversibly inhibits the activity of ATP2A2 in cardiac
CC       sarcoplasmic reticulum by decreasing the apparent affinity of the
CC       ATPase for Ca(2+). Modulates the contractility of the heart muscle in
CC       response to physiological stimuli via its effects on ATP2A2. Modulates
CC       calcium re-uptake during muscle relaxation and plays an important role
CC       in calcium homeostasis in the heart muscle. The degree of ATP2A2
CC       inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is
CC       alleviated by PLN phosphorylation (By similarity).
CC       {ECO:0000250|UniProtKB:P26678}.
CC   -!- SUBUNIT: Homopentamer. {ECO:0000250|UniProtKB:P26678}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:P26678}; Single-pass membrane protein
CC       {ECO:0000255}. Sarcoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:P26678}; Single-pass membrane protein
CC       {ECO:0000255}. Mitochondrion membrane {ECO:0000250|UniProtKB:A4IFH6};
CC       Single-pass membrane protein {ECO:0000255}. Membrane
CC       {ECO:0000250|UniProtKB:P61014}; Single-pass membrane protein
CC       {ECO:0000255}.
CC   -!- TISSUE SPECIFICITY: Heart. {ECO:0000269|PubMed:1825996}.
CC   -!- PTM: Phosphorylated in response to beta-adrenergic stimulation.
CC       Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated
CC       calcium uptake (By similarity). {ECO:0000250|UniProtKB:P26678}.
CC   -!- SIMILARITY: Belongs to the phospholamban family. {ECO:0000305}.
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DR   EMBL; M59039; AAA62738.1; -; mRNA.
DR   EMBL; M59037; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; M59038; AAA63167.1; -; Genomic_DNA.
DR   PIR; A39535; A39535.
DR   RefSeq; NP_990741.1; NM_205410.1.
DR   AlphaFoldDB; P26677; -.
DR   SMR; P26677; -.
DR   STRING; 9031.ENSGALP00000042569; -.
DR   PaxDb; P26677; -.
DR   GeneID; 396378; -.
DR   KEGG; gga:396378; -.
DR   CTD; 5350; -.
DR   eggNOG; ENOG502S97F; Eukaryota.
DR   InParanoid; P26677; -.
DR   OrthoDB; 1643351at2759; -.
DR   PhylomeDB; P26677; -.
DR   PRO; PR:P26677; -.
DR   Proteomes; UP000000539; Unplaced.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016529; C:sarcoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0033017; C:sarcoplasmic reticulum membrane; ISS:UniProtKB.
DR   GO; GO:0042030; F:ATPase inhibitor activity; IBA:GO_Central.
DR   GO; GO:1901895; P:negative regulation of ATPase-coupled calcium transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:1902081; P:negative regulation of calcium ion import into sarcoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0010459; P:negative regulation of heart rate; IBA:GO_Central.
DR   GO; GO:0051924; P:regulation of calcium ion transport; ISS:UniProtKB.
DR   CDD; cd20250; Phospholamban; 1.
DR   InterPro; IPR005984; PLB.
DR   PANTHER; PTHR21194; PTHR21194; 1.
DR   Pfam; PF04272; Phospholamban; 1.
DR   PIRSF; PIRSF001665; PLB; 1.
DR   TIGRFAMs; TIGR01294; P_lamban; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Endoplasmic reticulum; Membrane; Mitochondrion;
KW   Phosphoprotein; Reference proteome; Sarcoplasmic reticulum; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..52
FT                   /note="Cardiac phospholamban"
FT                   /id="PRO_0000191249"
FT   TOPO_DOM        1..31
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         16
FT                   /note="Phosphoserine; by PKA"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         17
FT                   /note="Phosphothreonine; by CaMK"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   52 AA;  6091 MW;  F95F86C36A0C4D3B CRC64;
     MEKVQYITRS ALRRASTLEV NPQARQRLQE LFVNFCLILI CLLLICIIVM LL