PPLA_RAT
ID PPLA_RAT Reviewed; 52 AA.
AC P61016; P20006;
DT 26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2004, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Cardiac phospholamban {ECO:0000305};
DE Short=PLB;
GN Name=Pln {ECO:0000312|EMBL:AAA41849.1};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1445334; DOI=10.1016/0006-291x(92)91144-f;
RA Johns D.C., Feldman A.M.;
RT "Identification of a highly conserved region at the 5' flank of the
RT phospholamban gene.";
RL Biochem. Biophys. Res. Commun. 188:927-933(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8508530; DOI=10.1161/01.res.73.1.193;
RA Shanahan C.M., Weissberg P.L., Metcalfe J.C.;
RT "Isolation of gene markers of differentiated and proliferating vascular
RT smooth muscle cells.";
RL Circ. Res. 73:193-204(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Aortic smooth muscle;
RX PubMed=1725098; DOI=10.1007/978-1-4684-6003-2_31;
RA Hwang K.S., Nadal-Ginard B.;
RT "Cloning phospholamban cDNA from rat aortic smooth muscle.";
RL Adv. Exp. Med. Biol. 304:387-395(1991).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16 AND THR-17, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Reversibly inhibits the activity of ATP2A2 in cardiac
CC sarcoplasmic reticulum by decreasing the apparent affinity of the
CC ATPase for Ca(2+). Modulates the contractility of the heart muscle in
CC response to physiological stimuli via its effects on ATP2A2. Modulates
CC calcium re-uptake during muscle relaxation and plays an important role
CC in calcium homeostasis in the heart muscle. The degree of ATP2A2
CC inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is
CC alleviated by PLN phosphorylation (By similarity).
CC {ECO:0000250|UniProtKB:P26678}.
CC -!- SUBUNIT: Homopentamer. Interacts with HAX1. Interact with ATP2A2; the
CC inhibition decreases ATP2A2 Ca(2+) affinity. Interacts with VMP1; VMP1
CC competes with PLN and SLN to prevent them from forming an inhibitory
CC complex with ATP2A2. Interacts with S100A1 in a Ca(2+)-dependent
CC manner. {ECO:0000250|UniProtKB:P26678}.
CC -!- INTERACTION:
CC P61016; Q6JP77: Akap7; NbExp=6; IntAct=EBI-7620725, EBI-6096191;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:P26678}; Single-pass membrane protein
CC {ECO:0000255}. Sarcoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:P26678}; Single-pass membrane protein
CC {ECO:0000255}. Mitochondrion membrane {ECO:0000250|UniProtKB:A4IFH6};
CC Single-pass membrane protein {ECO:0000255}. Membrane
CC {ECO:0000250|UniProtKB:P61014}; Single-pass membrane protein
CC {ECO:0000255}. Note=Colocalizes with HAX1 at the endoplasmic reticulum.
CC Colocalizes with DMPK a the sarcoplasmic reticulum.
CC {ECO:0000250|UniProtKB:P26678}.
CC -!- TISSUE SPECIFICITY: Heart. {ECO:0000269|PubMed:1725098}.
CC -!- PTM: Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated
CC calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to
CC beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate
CC sarcoplasmic reticulum calcium uptake in cardiomyocytes (By
CC similarity). {ECO:0000250|UniProtKB:P26678}.
CC -!- PTM: Palmitoylated by ZDHHC16, promoting formation of the homopentamer.
CC {ECO:0000250|UniProtKB:P61014}.
CC -!- SIMILARITY: Belongs to the phospholamban family. {ECO:0000305}.
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DR EMBL; L03382; AAA41849.1; -; Genomic_DNA.
DR EMBL; X71068; CAA50394.1; -; mRNA.
DR EMBL; S95849; AAN86727.1; -; mRNA.
DR EMBL; S95853; AAB21903.1; -; mRNA.
DR PIR; S37638; S37638.
DR RefSeq; NP_073198.1; NM_022707.2.
DR AlphaFoldDB; P61016; -.
DR BMRB; P61016; -.
DR SMR; P61016; -.
DR BioGRID; 249188; 2.
DR IntAct; P61016; 1.
DR MINT; P61016; -.
DR STRING; 10116.ENSRNOP00000000469; -.
DR iPTMnet; P61016; -.
DR PhosphoSitePlus; P61016; -.
DR PaxDb; P61016; -.
DR PRIDE; P61016; -.
DR GeneID; 64672; -.
DR KEGG; rno:64672; -.
DR UCSC; RGD:619894; rat.
DR CTD; 5350; -.
DR RGD; 619894; Pln.
DR eggNOG; ENOG502S97F; Eukaryota.
DR HOGENOM; CLU_214576_0_0_1; -.
DR InParanoid; P61016; -.
DR OMA; FTNFCLI; -.
DR TreeFam; TF330750; -.
DR Reactome; R-RNO-5578775; Ion homeostasis.
DR Reactome; R-RNO-936837; Ion transport by P-type ATPases.
DR PRO; PR:P61016; -.
DR Proteomes; UP000002494; Chromosome 20.
DR Bgee; ENSRNOG00000000413; Expressed in heart and 19 other tissues.
DR Genevisible; P61016; RN.
DR GO; GO:0090534; C:calcium ion-transporting ATPase complex; ISO:RGD.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISO:RGD.
DR GO; GO:0016020; C:membrane; ISO:RGD.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0032991; C:protein-containing complex; IDA:RGD.
DR GO; GO:0016529; C:sarcoplasmic reticulum; IDA:RGD.
DR GO; GO:0033017; C:sarcoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0031982; C:vesicle; IDA:RGD.
DR GO; GO:0042030; F:ATPase inhibitor activity; IDA:RGD.
DR GO; GO:0042802; F:identical protein binding; IPI:RGD.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0086023; P:adenylate cyclase-activating adrenergic receptor signaling pathway involved in heart process; ISO:RGD.
DR GO; GO:0006816; P:calcium ion transport; IMP:RGD.
DR GO; GO:0048738; P:cardiac muscle tissue development; ISO:RGD.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; ISO:RGD.
DR GO; GO:0032780; P:negative regulation of ATP-dependent activity; ISO:RGD.
DR GO; GO:1901895; P:negative regulation of ATPase-coupled calcium transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:1901877; P:negative regulation of calcium ion binding; ISO:RGD.
DR GO; GO:1902081; P:negative regulation of calcium ion import into sarcoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:1901020; P:negative regulation of calcium ion transmembrane transporter activity; ISO:RGD.
DR GO; GO:0051926; P:negative regulation of calcium ion transport; ISO:RGD.
DR GO; GO:0045822; P:negative regulation of heart contraction; ISO:RGD.
DR GO; GO:0010459; P:negative regulation of heart rate; ISO:RGD.
DR GO; GO:0007219; P:Notch signaling pathway; ISO:RGD.
DR GO; GO:1901894; P:regulation of ATPase-coupled calcium transmembrane transporter activity; ISO:RGD.
DR GO; GO:0090279; P:regulation of calcium ion import; ISO:RGD.
DR GO; GO:0051924; P:regulation of calcium ion transport; ISS:UniProtKB.
DR GO; GO:0086004; P:regulation of cardiac muscle cell contraction; ISO:RGD.
DR GO; GO:0010881; P:regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion; ISO:RGD.
DR GO; GO:0008016; P:regulation of heart contraction; ISO:RGD.
DR GO; GO:1901077; P:regulation of relaxation of muscle; ISO:RGD.
DR GO; GO:0010880; P:regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum; ISO:RGD.
DR GO; GO:0060314; P:regulation of ryanodine-sensitive calcium-release channel activity; ISO:RGD.
DR GO; GO:0002026; P:regulation of the force of heart contraction; ISO:RGD.
DR GO; GO:0086092; P:regulation of the force of heart contraction by cardiac conduction; ISO:RGD.
DR GO; GO:0032868; P:response to insulin; IEP:RGD.
DR GO; GO:0033574; P:response to testosterone; IDA:RGD.
DR GO; GO:0010043; P:response to zinc ion; IDA:RGD.
DR CDD; cd20250; Phospholamban; 1.
DR InterPro; IPR005984; PLB.
DR PANTHER; PTHR21194; PTHR21194; 1.
DR Pfam; PF04272; Phospholamban; 1.
DR PIRSF; PIRSF001665; PLB; 1.
DR TIGRFAMs; TIGR01294; P_lamban; 1.
PE 1: Evidence at protein level;
KW Acetylation; Endoplasmic reticulum; Lipoprotein; Membrane; Mitochondrion;
KW Palmitate; Phosphoprotein; Reference proteome; Sarcoplasmic reticulum;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..52
FT /note="Cardiac phospholamban"
FT /id="PRO_0000191248"
FT TOPO_DOM 1..31
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:A4IFH6"
FT MOD_RES 16
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 17
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT LIPID 36
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000250|UniProtKB:P61014"
SQ SEQUENCE 52 AA; 6095 MW; 0763601F76A854D3 CRC64;
MEKVQYLTRS AIRRASTIEM PQQARQNLQN LFINFCLILI CLLLICIIVM LL
