PPM1J_HUMAN
ID PPM1J_HUMAN Reviewed; 505 AA.
AC Q5JR12; B3KSB7; Q6DKJ7; Q96EZ7; Q9UF84;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Protein phosphatase 1J;
DE EC=3.1.3.16;
DE AltName: Full=Protein phosphatase 2C isoform zeta;
DE Short=PP2C-zeta;
GN Name=PPM1J; Synonyms=PPP2CZ;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT ILE-236.
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANT ILE-236.
RC TISSUE=Testis;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT ILE-236.
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT ILE-236.
RC TISSUE=B-cell, and Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-76, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66 AND SER-76, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] +
CC phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:83421; EC=3.1.3.16;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:61977; EC=3.1.3.16;
CC -!- SUBUNIT: Interacts with UBE2I/UBC9. {ECO:0000250}.
CC -!- INTERACTION:
CC Q5JR12; Q8TCE9: LGALS14; NbExp=3; IntAct=EBI-13292717, EBI-10274069;
CC Q5JR12; Q494U1-3: PLEKHN1; NbExp=3; IntAct=EBI-13292717, EBI-12014286;
CC Q5JR12; Q96GM5: SMARCD1; NbExp=3; IntAct=EBI-13292717, EBI-358489;
CC Q5JR12; Q9NZD8: SPG21; NbExp=3; IntAct=EBI-13292717, EBI-742688;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5JR12-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5JR12-2; Sequence=VSP_025882, VSP_025883;
CC -!- SIMILARITY: Belongs to the PP2C family. {ECO:0000305}.
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DR EMBL; AK093270; BAG52679.1; -; mRNA.
DR EMBL; AL133644; CAB63764.1; -; mRNA.
DR EMBL; AL603832; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471122; EAW56543.1; -; Genomic_DNA.
DR EMBL; BC011803; AAH11803.2; -; mRNA.
DR EMBL; BC073828; AAH73828.1; -; mRNA.
DR CCDS; CCDS855.2; -. [Q5JR12-1]
DR PIR; T43442; T43442.
DR RefSeq; NP_005158.5; NM_005167.5. [Q5JR12-1]
DR AlphaFoldDB; Q5JR12; -.
DR SMR; Q5JR12; -.
DR BioGRID; 130612; 33.
DR ELM; Q5JR12; -.
DR IntAct; Q5JR12; 22.
DR MINT; Q5JR12; -.
DR STRING; 9606.ENSP00000308926; -.
DR DEPOD; PPM1J; -.
DR iPTMnet; Q5JR12; -.
DR PhosphoSitePlus; Q5JR12; -.
DR BioMuta; PPM1J; -.
DR DMDM; 74741784; -.
DR EPD; Q5JR12; -.
DR jPOST; Q5JR12; -.
DR MassIVE; Q5JR12; -.
DR PaxDb; Q5JR12; -.
DR PeptideAtlas; Q5JR12; -.
DR PRIDE; Q5JR12; -.
DR ProteomicsDB; 63067; -. [Q5JR12-1]
DR ProteomicsDB; 63068; -. [Q5JR12-2]
DR Antibodypedia; 33835; 86 antibodies from 21 providers.
DR DNASU; 333926; -.
DR Ensembl; ENST00000309276.11; ENSP00000308926.6; ENSG00000155367.16. [Q5JR12-1]
DR Ensembl; ENST00000464951.1; ENSP00000475711.1; ENSG00000155367.16. [Q5JR12-2]
DR GeneID; 333926; -.
DR KEGG; hsa:333926; -.
DR MANE-Select; ENST00000309276.11; ENSP00000308926.6; NM_005167.7; NP_005158.5.
DR UCSC; uc001ecs.2; human. [Q5JR12-1]
DR CTD; 333926; -.
DR DisGeNET; 333926; -.
DR GeneCards; PPM1J; -.
DR HGNC; HGNC:20785; PPM1J.
DR HPA; ENSG00000155367; Group enriched (epididymis, skeletal muscle, skin, testis, tongue).
DR MIM; 609957; gene.
DR neXtProt; NX_Q5JR12; -.
DR OpenTargets; ENSG00000155367; -.
DR PharmGKB; PA142671150; -.
DR VEuPathDB; HostDB:ENSG00000155367; -.
DR eggNOG; KOG1323; Eukaryota.
DR GeneTree; ENSGT00940000160965; -.
DR HOGENOM; CLU_029072_0_0_1; -.
DR InParanoid; Q5JR12; -.
DR OMA; TAFTQMD; -.
DR OrthoDB; 601888at2759; -.
DR PhylomeDB; Q5JR12; -.
DR TreeFam; TF314700; -.
DR PathwayCommons; Q5JR12; -.
DR SignaLink; Q5JR12; -.
DR SIGNOR; Q5JR12; -.
DR BioGRID-ORCS; 333926; 30 hits in 1070 CRISPR screens.
DR ChiTaRS; PPM1J; human.
DR GenomeRNAi; 333926; -.
DR Pharos; Q5JR12; Tdark.
DR PRO; PR:Q5JR12; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q5JR12; protein.
DR Bgee; ENSG00000155367; Expressed in right testis and 95 other tissues.
DR ExpressionAtlas; Q5JR12; baseline and differential.
DR Genevisible; Q5JR12; HS.
DR GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0006470; P:protein dephosphorylation; IBA:GO_Central.
DR CDD; cd00143; PP2Cc; 1.
DR Gene3D; 3.60.40.10; -; 1.
DR InterPro; IPR015655; PP2C.
DR InterPro; IPR036457; PPM-type_dom_sf.
DR InterPro; IPR001932; PPM-type_phosphatase_dom.
DR PANTHER; PTHR13832; PTHR13832; 1.
DR Pfam; PF00481; PP2C; 2.
DR SMART; SM00332; PP2Cc; 1.
DR SUPFAM; SSF81606; SSF81606; 1.
DR PROSITE; PS51746; PPM_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Hydrolase; Phosphoprotein; Protein phosphatase;
KW Reference proteome.
FT CHAIN 1..505
FT /note="Protein phosphatase 1J"
FT /id="PRO_0000289058"
FT DOMAIN 104..498
FT /note="PPM-type phosphatase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01082"
FT REGION 1..103
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 66
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 76
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:23186163"
FT VAR_SEQ 1..167
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_025882"
FT VAR_SEQ 185..223
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_025883"
FT VARIANT 213
FT /note="L -> F (in dbSNP:rs34611728)"
FT /id="VAR_032566"
FT VARIANT 236
FT /note="V -> I (in dbSNP:rs10857971)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:15489334, ECO:0000269|PubMed:17974005,
FT ECO:0000269|Ref.4"
FT /id="VAR_032567"
SQ SEQUENCE 505 AA; 54834 MW; 3A2329219D5823BF CRC64;
MLNRVRSAVA HLVSSGGAPP PRPKSPDLPN AASAPPAAAP EAPRSPPAKA GSGSATPAKA
VEARASFSRP TFLQLSPGGL RRADDHAGRA VQSPPDTGRR LPWSTGYAEV INAGKSRHNE
DQACCEVVYV EGRRSVTGVP REPSRGQGLC FYYWGLFDGH AGGGAAEMAS RLLHRHIREQ
LKDLVEILQD PSPPPLCLPT TPGTPDSSDP SHLLGPQSCW SSQKEVSHES LVVGAVENAF
QLMDEQMARE RRGHQVEGGC CALVVIYLLG KVYVANAGDS RAIIVRNGEI IPMSREFTPE
TERQRLQLLG FLKPELLGSE FTHLEFPRRV LPKELGQRML YRDQNMTGWA YKKIELEDLR
FPLVCGEGKK ARVMATIGVT RGLGDHSLKV CSSTLPIKPF LSCFPEVRVY DLTQYEHCPD
DVLVLGTDGL WDVTTDCEVA ATVDRVLSAY EPNDHSRYTA LAQALVLGAR GTPRDRGWRL
PNNKLGSGDD ISVFVIPLGG PGSYS
