PPM1M_MOUSE
ID PPM1M_MOUSE Reviewed; 462 AA.
AC Q8BU27; E9Q2I3; Q9CSD6; Q9CU88;
DT 02-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT 29-SEP-2021, sequence version 3.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Protein phosphatase 1M {ECO:0000305};
DE EC=3.1.3.16 {ECO:0000255|PROSITE-ProRule:PRU01082};
DE AltName: Full=Protein phosphatase 2C isoform eta;
DE Short=PP2C-eta;
DE Short=PP2CE;
GN Name=Ppm1m {ECO:0000312|MGI:MGI:1915155}; Synonyms=Ppm1e;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000312|EMBL:BAC40085.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RC TISSUE=Embryo {ECO:0000312|EMBL:AAR01612.1};
RX PubMed=14654243; DOI=10.1016/j.bbaexp.2003.09.004;
RA Komaki K., Katsura K., Ohnishi M., Li M.G., Sasaki M., Watanabe M.,
RA Kobayashi T., Tamura S.;
RT "Molecular cloning of PP2Ceta, a novel member of the protein phosphatase 2C
RT family.";
RL Biochim. Biophys. Acta 1630:130-137(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 10-462 (ISOFORM 3).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Corpora quadrigemina, Head, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] +
CC phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:83421; EC=3.1.3.16; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU01082};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:61977; EC=3.1.3.16; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU01082};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU01082};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU01082};
CC Note=Binds 2 magnesium or manganese ions per subunit.
CC {ECO:0000255|PROSITE-ProRule:PRU01082};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14654243}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=3;
CC IsoId=Q8BU27-3; Sequence=Displayed;
CC Name=1 {ECO:0000269|PubMed:14654243};
CC IsoId=Q8BU27-1; Sequence=VSP_061129;
CC Name=2 {ECO:0000305};
CC IsoId=Q8BU27-2; Sequence=VSP_061128;
CC -!- TISSUE SPECIFICITY: Widely expressed with highest levels in testis and
CC lower levels in lung, kidney and brain. {ECO:0000269|PubMed:14654243}.
CC -!- SIMILARITY: Belongs to the PP2C family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY332616; AAR01612.1; -; mRNA.
DR EMBL; AK013149; BAB28679.2; -; mRNA.
DR EMBL; AK017245; BAB30649.1; -; mRNA.
DR EMBL; AK046387; BAC32699.1; -; mRNA.
DR EMBL; AK087999; BAC40085.1; -; mRNA.
DR EMBL; AC164430; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS23471.1; -. [Q8BU27-1]
DR CCDS; CCDS23472.2; -. [Q8BU27-3]
DR RefSeq; NP_945149.2; NM_198931.3.
DR AlphaFoldDB; Q8BU27; -.
DR SMR; Q8BU27; -.
DR STRING; 10090.ENSMUSP00000117908; -.
DR PhosphoSitePlus; Q8BU27; -.
DR MaxQB; Q8BU27; -.
DR PaxDb; Q8BU27; -.
DR PRIDE; Q8BU27; -.
DR ProteomicsDB; 291716; -. [Q8BU27-1]
DR ProteomicsDB; 291717; -. [Q8BU27-2]
DR ProteomicsDB; 364011; -.
DR Antibodypedia; 31173; 172 antibodies from 24 providers.
DR DNASU; 67905; -.
DR GeneID; 67905; -.
DR KEGG; mmu:67905; -.
DR UCSC; uc012gzs.1; mouse.
DR CTD; 132160; -.
DR MGI; MGI:1915155; Ppm1m.
DR VEuPathDB; HostDB:ENSMUSG00000020253; -.
DR eggNOG; KOG1323; Eukaryota.
DR HOGENOM; CLU_029072_2_0_1; -.
DR InParanoid; Q8BU27; -.
DR OMA; QATCCQI; -.
DR OrthoDB; 601888at2759; -.
DR PhylomeDB; Q8BU27; -.
DR TreeFam; TF314700; -.
DR BioGRID-ORCS; 67905; 3 hits in 74 CRISPR screens.
DR ChiTaRS; Ppm1m; mouse.
DR PRO; PR:Q8BU27; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q8BU27; protein.
DR Bgee; ENSMUSG00000020253; Expressed in saccule of membranous labyrinth and 247 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0030145; F:manganese ion binding; IDA:UniProtKB.
DR GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0004721; F:phosphoprotein phosphatase activity; IDA:MGI.
DR GO; GO:0006470; P:protein dephosphorylation; IDA:UniProtKB.
DR CDD; cd00143; PP2Cc; 1.
DR Gene3D; 3.60.40.10; -; 1.
DR InterPro; IPR015655; PP2C.
DR InterPro; IPR036457; PPM-type_dom_sf.
DR InterPro; IPR001932; PPM-type_phosphatase_dom.
DR PANTHER; PTHR13832; PTHR13832; 1.
DR Pfam; PF00481; PP2C; 2.
DR SMART; SM00331; PP2C_SIG; 1.
DR SMART; SM00332; PP2Cc; 1.
DR SUPFAM; SSF81606; SSF81606; 1.
DR PROSITE; PS51746; PPM_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Hydrolase; Magnesium; Manganese; Metal-binding;
KW Nucleus; Protein phosphatase; Reference proteome.
FT CHAIN 1..462
FT /note="Protein phosphatase 1M"
FT /id="PRO_0000057757"
FT DOMAIN 100..452
FT /note="PPM-type phosphatase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01082"
FT REGION 1..66
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..28
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 127
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:P35813"
FT BINDING 127
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:P35813"
FT BINDING 128
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:P35813"
FT VAR_SEQ 1..158
FT /note="MSAGWFRRRFLPGGPLPEPRPAGPRSSPVPYHRPRFLRGSGSSPGATDASRR
FT PDARPVRSPARGRTLPWNAGYAEVINAEKSEFNEDQAACGKLCIRRCEFGIEEDQEWLT
FT VCPEEFLTGHYWALFDGHGGPAAAILAANTLHSCLRRQLEAVVEGMI -> MM (in
FT isoform 2)"
FT /id="VSP_061128"
FT VAR_SEQ 1..75
FT /note="MSAGWFRRRFLPGGPLPEPRPAGPRSSPVPYHRPRFLRGSGSSPGATDASRR
FT PDARPVRSPARGRTLPWNAGYAE -> MYVPPRTSLRVWPMLCGIR (in isoform
FT 1)"
FT /id="VSP_061129"
FT CONFLICT 10
FT /note="F -> V (in Ref. 2; BAB28679)"
FT /evidence="ECO:0000305"
FT CONFLICT 106
FT /note="D -> H (in Ref. 2; BAC32699/BAB28679)"
FT /evidence="ECO:0000305"
FT CONFLICT 158
FT /note="I -> M (in Ref. 1; AAR01612 and 2; BAC40085)"
FT /evidence="ECO:0000305"
FT CONFLICT 212
FT /note="S -> L (in Ref. 2; BAB30649)"
FT /evidence="ECO:0000305"
FT CONFLICT 279
FT /note="F -> L (in Ref. 2; BAB30649)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 462 AA; 51204 MW; 55316E77217C5C5D CRC64;
MSAGWFRRRF LPGGPLPEPR PAGPRSSPVP YHRPRFLRGS GSSPGATDAS RRPDARPVRS
PARGRTLPWN AGYAEVINAE KSEFNEDQAA CGKLCIRRCE FGIEEDQEWL TVCPEEFLTG
HYWALFDGHG GPAAAILAAN TLHSCLRRQL EAVVEGMIAP QPPMHLSGRC VCPSDPQFVE
EKGIQAEDLV IGALENAFQE CDDVIGRELE ASGQVGGCTA LVAVFLQGKL YVANAGDSRA
ILVRRHEIRQ LSSEFTPETE RQRIQQLAFT YPELLAGEFT RLEFPRRLKG DDLGQKVLFR
DHHMRGWSYK RVEKSDLKYP LIHGQGRQAR LLGTLAVSRG LGDHQLRVLD TDIQLKPFLL
SIPQVTVLDV HQLAVQEEDV VVMATDGLWD VLSNEQVALL VRSFLTGNQK DDPHRFSELA
KMLIHNTQGK DNGATGEGQV SYDDVSVFVI PLHSQAQEGS GH
